摘要:

Metallomicspublishes original research and topical reviews, which provide insight into the role of metals in the life sciences. This includes:• Chemical speciation, dynamics and kinetics of trace elements in biological systems• Elemental distributions and concentrations linked to the genome• Regulation of the uptake, accumulation and metabolism of metals and other trace elements in biological systems• Physiological and pathological mechanisms related to trace elements in human health and disease• Structural analysis and coregulation of elements within the metallome, including the survey and identification of metalloproteins/enzymes• Genetic and molecular genetic basis for regulation of metallomes and epigenetic factors relative to the organism• The interaction of metallodrugs, including chemotherapy agents, with biological organisms, including in clinical use• Metal exchange between biota and the environment• Bioimaging and biosensing of metals, including analysis of diagnostic and therapeutic radioactive metals• Certified reference materials for biological applicationsReadership will be cross-disciplinary and include researchers in academia and industry working in analytical science, biogeochemistry, bioinformatics, biological catalysis, biological environmental science, cell biology, clinical chemistry, environmental health, medicine, metallobiochemistry, microbiology, nutritional chemistry, pharmacology, plant biochemistry and physiology and toxicology.

ISSN:1756-5901    

DOI:10.1039/b926378k

出版商:RSC    

年代:2009

数据来源: RSC

摘要:

Happy New Year and welcome toMetallomicsissue 1, 2010. We have had a very exciting first year where we have had the opportunity to bring you both primary research and review articles showing the breadth of research into trace elements in the life sciences. Topics have ranged from protein–receptor interactions in iron transport (Alvin L. Crumblisset al.,Metallomics, 2009,1, 249, DOI:10.1039/b902860a) to the tungsten metallome ofPyrococcus furiosus(Wilfred R. Hagenet al.,Metallomics, 2009,1, 395, DOI:10.1039/b908175e), while our reviews have given insight into areas such as inhibition of transcription by platinum antitumor compounds (Ryan C. Todd and Stephen J. Lippard,Metallomics, 2009,1, 280, DOI:10.1039/b907567d) and copper metabolism (Hiroko Kodama and Chie Fujisawa,Metallomics, 2009,1, 42, DOI:10.1039/b816011m).We hope that you have enjoyed reading these articles and seeing the diverse backgrounds of those engaged in metallomics research. This has helped us to take the first steps to achieve the goal that we set in our first issue—to bridge the gap between researchers from different backgrounds so that ideas can be shared and the field progresses to the benefit of all.Reflecting the importance of this area of research, the growth of the field and the growth of this community, we are delighted to let you know thatMetallomicswill be published monthly this year. We hope that you will enjoy reading the journal and will also submit some of your best research. To submit your article, just visit our websitewww.rsc.org/metallomics.Don’t forget that content inMetallomicscontinues to be free to access this year. The current issue is freely available from our website and back issues are available to institutional subscribers following a simple registration process. You can register atwww.rsc.org/Publishing/Journals/free_access_registration.asp.

ISSN:1756-5901    

DOI:10.1039/b926104b

出版商:RSC    

年代:2009

数据来源: RSC

摘要:

Joe CarusoJoe Carusoholds a PhD from Michigan State University. After a one-year postdoctoral fellowship at the University of Texas-Austin, he joined the UC faculty and since then, he has authored or co-authored about 360 scientific publications and presented over 300 invited lectures at universities and at scientific meetings. He has more than 7000 citations to research papers and review articles since 1980. Caruso is a member of the American Chemical Society, Society for Applied Spectroscopy and a Fellow of the Royal Society of Chemistry and former Editorial Board Chair ofJAAS.His research interests have long involved atomic mass spectrometry, which led to elemental speciation studies and ultimately metallomics research. Current interests involve using atomic and molecular mass spectrometry to better understand cell toxicity from the molecular point of view contemporaneously with major cell events. His research on metals, non-metals and their differing forms has implications for chemical warfare agent detection, environmental remediation, and health care.He has been honored by Eastern Michigan University with its 1990 Distinguished Alumni Award, by the American Chemical Society with the 1992 Cincinnati Chemist of the Year Award, the Federation of Analytical Chemistry and Spectroscopy Society with the 1994 Anachem Award, and with the 2000 Spectrochemical Analysis Award given by the Analytical Division of the American Chemical Society. Recently he received the University of Cincinnati—Excellence in Doctoral Student Mentoring Award and the UC 2007 Rieveschl Excellence in Research Award.Ariel D. AnbarAriel D. Anbaris a biogeochemist in the Department of Chemistry and Biochemistry and the School of Earth & Space Exploration at Arizona State University. He received an AB in Geological Sciences and Chemistry from Harvard University in 1989, and a PhD in Geochemistry from the California Institute of Technology in 1996.Anbar’s research centers on the chemistry of transition elements in the environment. A major emphasis of this research is to determine how the abundances of bioessential elements such as Fe and Mo changed in the oceans during Earth history. The goal is to understand how these changes affected evolution.This research is pursued through the development and application of novel analytical approaches using ICP mass spectrometry, In particular, Anbar’s group pioneered the use of multiple-collector, magnetic sector ICP-MS to precisely measure mass-dependent variations in the isotopic abundances of transition elements that arise from mass fractionation. This work reveals that abundance variations of 0.01–0.1%/amu, once undetectable, are ubiquitous in nature. Such measurements in natural samples, informed by laboratory experiments and theoretical studies, provide insights into the environmental chemistry of metals and the metal-centered interactions between organisms and their surroundings. Applied to the geologic record, such “metal stable isotope” studies provide information about metal biogeochemical cycles on the ancient Earth, environmental changes that perturbed these cycles, and biological activity in the distant past.Hiroki HaraguchiHiroki Haraguchigraduated from the University of Tokyo (1965), from which he also received MSci and PhD degrees in 1967 and 1973, respectively. Since 1969, he served as assistant professor in the University of Tokyo, research chemist in the National Institute for Environmental Studies, and associate professor in the University of Tokyo. From 1975 to 1977, he was a postdoctoral fellow at the University of Florida, where he worked with Prof. J. D. Winefordner. He was promoted to full professor of analytical chemistry laboratory in the Department of Applied Chemistry of Nagoya University in 1988, and retired from there in 2007. He is currently Emeritus Professor of Nagoya University as well as the program officer of the Ministry of Environment and the associate member of Science Council of Japan. His research interests are the development of highly-sensitive analytical methods for chemical speciation of trace elements in biological, environmental and geochemical systems. He proposed “metallomics as integrated biometal science” inJAASin 2004, which has been receiving great attention as a newly emerging scientific field in life science, complementary to genomics and proteomics. Furthermore, he has been attempting to establish the concept of “cell microcosm”, which indicates the existence of all elements in a single biological cell.Gary M. HieftjeGary M. Hieftjeis Distinguished Professor and Mann Chair of Chemistry at Indiana University in Bloomington, Indiana. His research interests include the investigation of basic mechanisms in atomic emission, absorption, fluorescence and mass spectrometric analysis, and the development of instrumentation and techniques for atomic methods of analysis. He is interested also in the on-line computer control of chemical instrumentation and experiments, the use of time-resolved luminescence processes for analysis, the application of information theory to analytical chemistry, analytical mass spectrometry, near-infrared reflectance analysis, metallomics, and the use of stochastic processes to extract basic and kinetic chemical information. He has won numerous awards in the fields of analytical chemistry and spectroscopy, has held major offices in several scientific societies, and has served on the Editorial Boards of many major journals. He is the author of over 500 publications, 10 books, and 15 patents. More than 65 students have received doctorates under his direction.Ryszard LobinskiRyszard Lobinskiis research director at the CNRS (Laboratory of Analytical Bioinorganic and Environmental Chemistry in Pau) and professor of chemistry at the Warsaw University of Technology. He obtained his PhD (1989) and DSc (habilitation) (1994) degrees from the Warsaw University of Technology, Poland. He held postdoctoral positions at the Institute of Spectroscopy and Applied Spectrometry (ISAS) in Dortmund (1990) and at the University of Antwerp (UIA) (1991–1994) before joining the National Research Council of France (CNRS) in 1994. R. Lobinski is the author or co-author of over 200 articles in international journals, 3 books, 3 edited journal issues, and about 90 invited lectures at international meetings. He received the CNRS 2006 Silver Medal and is a Fellow of the Royal Society of Chemistry. He is also the co-director of UltraTrace Analyses Aquitaine (UT2A) (a startup company at the University of Pau) and Past-President of the Analytical Chemistry Division of IUPAC. His principal research interest is the development of analytical approaches to species-specific (speciation) analysis for trace and ultratrace metals in environmental and nutrition chemistry and in life sciences.Thomas V. O’HalloranThomas V. O’Halloranis Morrison Professor, Northwestern University, Department of Chemistry and Department of Biochemistry, Molecular Biology and Cell Biology. He completed his PhD at Columbia University, New York, with Professor S. J. Lippard and followed this with a post-doctoral position at MIT with Professor C. T. Walsh.Professor O’Halloran’s research interests include the chemistry and biology of transition metal receptors; transcriptional regulation mechanisms; metalloregulatory proteins; molecular and cell biology of copper, iron and zinc; metal ion chaperone proteins for Cu and Zn; mercury thiolate and protein chemistry; high-valent, iron-oxo chemistry; non-heme iron enzymes; design and development of inorganic-based antitumor drugs and protease inhibitors.Professor O’Halloran’s awards and recognitions include: Member, Advisory Board, US DOE Biological Sciences Directorate; NIH MERIT Award (Method to Extend Research in Time) for grant “Mechanistic Studies of the MerR and Fur Metalloregulatory Proteins”; Advisory Board, Center for Environmental Bioinorganic Chemistry Princeton University; Robert Lurie Cancer Center at Northwestern University—Director of Cancer Genes and Molecular Regulation Basic Research Program; Leadership Council; Co-Director of Molecular Oncogenesis Basic Research Group; Fellow, American Association for the Advancement of Science; Fellow, John Simon Guggenheim Memorial Foundation; Leroy Hall Award for Excellence in Teaching given by the Weinberg College of Arts and Sciences; American Society of Biochemistry and Molecular Biology Scientific Achievement Award sponsored by Schering-Plough; Associated Student Government Faculty Honor Roll; Mortar Board—Faculty Honor Roll of Outstanding Teachers; The Camille and Henry Dreyfus Foundation Teacher-Scholar Award; Alfred P. Sloan Research Fellow; Presidential Young Investigator Award, NSF; National Searle Scholars Award, The Chicago Community Trust; The Camille and Henry Dreyfus Foundation Distinguished New Faculty Award; Pegram Award, Columbia University.David E. SaltDavid E. Saltis a Professor at Purdue University in the Department of Horticulture and Landscape Architecture, West Lafayette, Indiana, USA. His long term research interest is to understand the function of the genes and gene networks that regulate the plant ionome (elemental composition), along with the evolutionary forces that shape this regulation. To achieve this his research group couples high-throughput elemental profiling, with bioinformatics, genomics and genetics, in both genetic model species (yeast,Arabidopsisand rice) and “wild” plants (Astragalus,ThlaspiandPteris). He has been involved in such work since his PhD (Liverpool University, UK, 1985–1988) working on the mechanisms of copper tolerance inMimulus gutattus(yellow monkey flower). He also has a BSc in Biochemistry (University of North Wales, Bangor, UK, 1981–1984) and an MSc in Computer Science (Hallam University, UK, 1984–1985). He has published over 75 peer reviewed papers since 1989 with approximately 4000 citations. During his career, he has won competitive research funding from USDA, DOE, NSF and NIH. He is a member of the American Society of Plant Biologists and has sat on both the Education and Public Affairs committees. He is also a Monitoring Editor for Plant Physiology, and on the Editorial Boards of theBrazilian Journal of Plant Physiology,BMC Plant Biologyand theInternational Journal of Phytoremediation.Bibudhendra (Amu) SarkarBibudhendra (Amu) Sarkaris a leading authority in Inorganic Biochemistry. He graduated with a PhD degree in Biochemistry from the University of Southern California, Los Angeles in 1964. He joined the Department of Biochemistry, University of Toronto and Research Institute of the Hospital for Sick Children with a Medical Research Council Scholar award. He was a Visiting Scientist in the Institute de Biologie Physico-Chimique, Université de Paris-Sorbonne, France where he worked on theab initiomolecular orbital calculations of metal-binding sites of proteins. He was a Visiting Scholar working on protein structure in the Department of Biochemistry, University of Cambridge, UK with an award from the Nuffield Foundation. As the Head of Structural Biology and Biochemistry Department in the Hospital, he established a major research centre with a broad initiative in protein structure and function. He discovered the copper–histidine treatment of Menkes’ disease, a devastating neurodegenerative disease in children caused by genetic defect of copper transport. His pioneering research on the structure and function of metal-binding proteins led to the discovery of ATCUN motif, which can serve as a probe for protein structure determination by paramagnetic relaxation enhancement NMR spectroscopy and selective cleavage of DNA. His major research interests are in the area of metal-related genetic diseases with a special emphasis on Wilson’s and Menkes’ diseases and studying the effects of toxic metals in the environment and its impact on human health. Dr Sarkar is a Professor Emeritus in the University of Toronto and the Hospital for Sick Children, Toronto.Hongzhe SunHongzhe Sunobtained his PhD from the University of London (with Peter J. Sadler) in 1996. After postdoctoral work at the University of Edinburgh, he joined the Department of Chemistry at the University of Hong Kong in 1998 where he is currently a professor. He is the recipient of the NSFC Outstanding Young Scholar Award in 2005, and serves on the Advisory Board of theJournal of Biological Inorganic Chemistry. His research interests are centered at metallodrugs and metalloproteins, inorganic structural biology and metallomics.Nigel RobinsonNigel Robinson, King Edwards foundation scholar, studied life sciences at theUniversity of Liverpool, specialising in Botany, graduating with a first in 1981 and completing a doctorate in 1984 with David Thurman on the mechanism of copper tolerance inMimulus guttatus. Supported by Fellowships from theNatural Environment Research Council, andDirectors-office of Los Alamos National Laboratory, he worked with Paul Jackson atLANL(1984–1987) then held aRoyal Society University Research Fellowshipat Durham University, UK (1987–1994) to gather genetic resources for metal-homeostasis. He was awarded the Presidents medal of theSociety for Experimental Biologyin 1993 in recognition of distinguished work on metalloproteins and metal interactions with plant and microbial cells. In 1994 he was appointed to the chair of Genetics in the Medical School atNewcastle University. He has trained two-dozen postgraduates, writtencircaone hundred papers, served as Editorial Advisor toMolecular Microbiologyand theBiochemical Journal, organised a dozen metals-related conferences, delivered more than a hundred invited lectures and co-instigated (with Dr Dennis Winge of the University of Utah) theGordon Research Conferenceseries on theCell Biology of Metals. With nearly a half of enzymes estimated to need metals he has contributed to understanding how cells assist proteins to acquire the correct metals.Marco Aurélio Zezzi ArrudaMarco Aurélio Zezzi Arrudawas born in Piracicaba, São Paulo state, Brazil in 1965. In 1996 he assumed the position of Assistant Professor at the University of Campinas-Unicamp, Department of Analytical Chemistry, becoming an Associate Professor in 2001. The main research lines of his research group include mass and atomic spectrometry, sample preparation, mechanization and bioanalytical with emphasis on metallomics. In the field of metallomics his current research interests include interdisciplinary work involving comparative metallomics of plants (i.e.soybean, sunflower) and human body fluids (i.e.blood serum, saliva, urine) to identify possible biomarkers for transgenic species and human diseases, as well as to evaluate reactive oxygen species production, and (metallo)proteins responses under metallic stress in a given system.Ivano BertiniIvano Bertiniis Professor of Inorganic Chemistry at the University of Florence and is Director of the Magnetic Resonance Center (CERM). He received several honors, among which three Laurea Honoris Causa (from the universities of Stockholm, Ioannina and Siena). He is a member of the Academia Europaea and the Italian Accademia dei Lincei, and is, or has been on the Editorial staff or Advisory Board of over 20 of the most authoritative journals in chemistry, biochemistry and inorganic chemistry.Since 1975 he has studied the structure–function relationship of metalloproteins through biophysical methods. In 1990, he transformed his lab into an NMR lab for structural biology of metalloproteins, and eventually pioneered the exploitation of genome data banks. He has pursued advancements in technology for solution structure determination, particularly for paramagnetic metalloproteins, and developed specific software applications. He has also established a molecular biology department for high-throughput protein expression in structural genomics projects on metalloproteins. He has published over 600 research articles and has solved more than 100 protein structures.In 1999 he founded the present Magnetic Resonance Center (CERM) at the University of Florence in an independent and prestigious building hosting an impressive battery of NMR spectrometers. The Center constitutes a major NMR infrastructure in the Life Sciences.Jörg BettmerJörg Bettmerfinished his chemistry degree (University of Münster, Germany) in November 1992. Afterwards, he started at the Department of Analytical Chemistry at the same university under the supervision of Prof. K. Cammann his PhD which he finished in 1996. After several short-term post-doctoral stays he moved to the University of Mainz, Germany, in 2000, where he obtained his habilitation in summer 2004 under the supervision of Professor Klaus G. Heumann. After a visiting professorship for environmental and analytical chemistry at the Humboldt-University in Berlin in 2004/05 he moved back to Mainz, where he was appointed as assistant professor in analytical chemistry until 2007. In May 2007 he moved to the University of Oviedo, Spain (Professor Alfredo Sanz-Medel), where he is currently holding a position as “Ramón y Cajal researcher”.His research interests and experience are mainly centred in the field of elemental speciation with special emphasis on the development of hyphenated techniques. This includes labelling strategies for (quantitative) protein and metalloprotein determinations.Zhifang ChaiZhifang Chaiis a radiochemist working at Multidisciplinary Initiative Centre, Institute of High Energy Physics, the Chinese Academy of Sciences. He was graduated from Fudan University, China, in 1964. As a fellow of the Alexander von Humboldt Foundation, Germany, he worked at Cologne University from 1980 to 1982 in the field of nuclear technology and its applications. Later, he worked in France, USA, the Netherlands and Japan. He has long been involved in the methodology of nuclear analytical techniques and their multidisciplinary applications, especially in study of the chemical speciation of trace elements in environmental and biological systems. He authored or co-authored over 332 papers in peer-review journals, 6 Chinese books and 3 English books. He is a member of many domestic and international scientific societies. In 2005 he was awarded the George von Hevesy Award—the premier international award of excellence to honor outstanding achievements in radioanalytical and nuclear chemistry. In 2007 he was elected as a member of the Chinese Academy of Sciences. His present interest is to develop novel nuclear analytical methods for metallomics study.Heidi Goenaga-InfanteHeidi Goenaga-Infantegraduated from the University of Havana, Cuba and then was awarded a MUTIS fellowship from the Spanish International Cooperation Agency at the University of Oviedo, Spain from which she obtained her PhD in 1999. She was a research assistant at the Micro and Trace Analysis Centre (MiTAC), University of Antwerp from 1999–2003. In 2003, she joined LGC’s mass spectrometry team in Teddington, United Kingdom as a senior research and development scientist.Dr Heidi Goenaga-Infante is currently the lead scientist of speciation analysis and metallomics research at LGC. Her research interests concern the combined application of elemental and molecular mass spectrometry with chromatography for heteroatom speciation analysis of dietary substances, supplements and clinical samples and the high accuracy determination of heteroatom-containing species in these substances by IDMS approaches with the aim to characterise new “speciated” certified reference materials. She has undertaken and managed a wide variety of projects and studies including the coordination of CCQM (Consultative Committee on Amount of Substance) international intercomparisons on the speciation field.Jose Luis Gómez-ArizaJose Luis Gómez-Arizais full professor of Analytical Chemistry at the University of Huelva. He received his BSc (1973) and PhD (1976) from the University of Sevilla. He was temporary assistant professor (1973–77) and permanent assistant professor (1977–93) at the University of Sevilla. In 1993 he moved to the University of Huelva where he became a full professor. His research interests include the investigation of new analytical approaches for chemical species characterization using hyphenated techniques and the analysis of metal species involved in environmental, food and health issues. The study of metalloproteins and other metal-linking biomolecules using multidimensional analytical approaches (metallomics) is now under his attention considering the integration with other -omics such as proteomics and transcriptomics. He is the author or co-author of over 250 scientific publications, 2 books, and holds several patents. Over 25 students have received doctorates under his direction; many others have received MS degrees, and scores of undergraduates and visiting scientists have performed research in his laboratory.J. L. Gómez-Ariza is a member of the Spanish Society for Analytical Chemistry (SEQA), Royal Society of Chemistry, American Chemical Society and American Society for Biochemistry and Molecular Biology. He was honoured with the Andalusia Research Award in 2003 and the Huelva Industry Excellence Research Award also in 2003. He was president of Speciation Group of SEQA and the Andalusia Group of the Spanish Society for Analytical Chemistry (GRASEQA). In 2003 was appointed as an academic of the Academy of Sciences, Arts and Letters of Huelva.Rudi GrimmRudi Grimmreceived his PhD in Biology at the University of Munich in 1987. After completing a post-doc at the University of Freiburg, Germany and the Riken Institute in Tokyo, Japan he joined Hewlett-Packard as a senior life science application chemist in 1991. In 1998 he left the company and became the head of protein chemistry at the Munich based proteomics company Toplab. In June 1999 he joined Hexal Pharma to establish the biotech laboratories for the development of generic recombinant protein drugs. In September 2002 he rejoined Agilent Technologies as the worldwide proteomics and metabolomics market development manager. Since May 2006 he is working at the Agilent Technologies headquarter in Santa Clara, California.Norbert JakubowskiNorbert Jakubowskistudied physics at the University of Duisburg, Essen and completed his PhD in physics at the University of Hohenheim. He was a senior scientist at the Institute for Analytical Sciences, Dortmund and Berlin (from 1983 to 2009) and is now Head of BAM's Division I.1 Inorganic Chemical Analysis and Reference Materials (since July 2009). His research interests include inorganic trace and ultra trace analysis of liquids and solids by use of ICP-MS and GD-MS, elemental speciation analysis and development of elemental tags for quantitative detection of biomolecules. He has received awards including the Alan Date Memorial Award from VG Elemental, Surrey, GB (1990), Zimmer International Scholar from University of Cincinnati, USA (2005) and a Waters Symposium Award for Pioneers in ICP-MS (2006). Dr Jakubowski is a member of the Editorial Board ofJAAS(Reviews Editor since 2006) and of the Advisory Boards of the journals:Analytical and Bioanalytical ChemistryandAnalyst.David W. KoppenaalDavid W. Koppenaalis a Laboratory Fellow, Associate Director of the Biological Sciences Division, and Chief Technology Officer of EMSL at Pacific Northwest National Laboratory. Before joining the Advisory Board ofMetallomics, he has been a member of the Editorial Board ofJAASand is currently an Advisory Board member. Dr Koppenaal’s research interests include atomic mass spectrometry instrumentation and applications, with special interests in metallomic applications of ICP-MS, and collision/reaction cell, high-resolution MS, and new generation MS detection techniques. Dr Koppenaal is a Fellow of the Royal Society of Chemistry and the American Association for the Advancement of Science (AAAS).Peter M. H. KroneckPeter M. H. Kroneckholds a Diploma in Chemistry from Universität Basel, Switzerland, and completed his Dr rer. nat. studies at Universität Konstanz, Germany. He undertook postdoctoral studies at Utah State University, USA and his habilitation at Universität Konstanz, Germany.He is currently Professor of Biochemistry, Universität Konstanz (since 1986) and his professional experience includes Visiting Scientist, Natl. Biomed. ESR Center, Milwaukee, USA (1988–1994), Head Bioinorganic Research Group, Universität Konstanz (1980–1986), Visiting Scientist, Weizmann Institute of Science, Rehovot, Israel (1977), Visiting Scientist, University of Wisconsin Madison, USA (1976)His achievements include: Chair of the “Metals in Biology” GRC (2009); Organizer DAAD Summer SchoolMetals in Biology—Key Elements of Life, Cuernavaca, Mexico (2008); Member Natl. Org. Committee, EUROBIC 4, Garmisch-Partenkirchen (2004); Organizer/Chair, Intern. SymposiumCopper 2003, Konstanz (2003); LecturerMetals in Biological Systems, Center for Intern. Studies, Grinnell College, USA (2003); LecturerCu Enzymes and Proteins, Louvain-la-Neuve, Belgium (1985–2003); Medal of the European Society for Bioinorganic Chemistry, received at EUROBIC 6, Lund, Sweden (2002); Organizer/Co-Chair, 13th Intern. ConferenceFlavins and Flavoproteins, Konstanz (1999); Organizer/Co-chair, Intern. Volkswagen-SymposiumElectron Transfer, Konstanz (1999); Natl. Org. Committee of ICBIC 7, Lübeck (September 1995); Organizer/Chair, Intern. SymposiumFeS Proteins, Konstanz (1994); Organizer/Chair, Intern. MeetingRedox Cofactors and Metals in Proteins, Konstanz (1991).Maria Montes BayónMaria Montes Bayónis Associate Professor at the Department of Physical and Analytical Chemistry of Oviedo′s University since 2007. After completing her chemistry degree at the University of Oviedo (1993), she performed her MSc Studies at the University of Plymouth (UK) under the supervision of Dr Hywel Evans and Prof. Les Ebdon. Once back in Oviedo, she started her PhD studies Oviedo University under the supervision of Dr J. I. Garcia Alonso and Prof. A. Sanz Medel that concluded in 1999. The thesis was awarded with a Departmental Prize due to the number and quality of publications obtained. Afterwards, she conducted a post-doctoral work as Fulbright Scholar at the Department of Chemistry in the University of Cincinnati (Ohio, USA) for 26 months, under the supervision of Prof. J. A. Caruso. Back to Spain from USA, she obtained the “Ramón y Cajal” research contract (five years) and she joined the group leaded by Prof. Sanz-Medel. During this period, she obtained the national habilitation and then the permanent position at the Department of Physical and Analytical Chemistry.She is author or co-author of more than 70 scientific publications in international journals and book chapters. This work has been presented in many lectures at national and international Conferences also as invited speaker. Her main research topics include hybrid techniques for speciation to solve biological and environmental problems. HPLC, Capillary GC and Capillary Electrophoresis, as powerful separation tools are coupled to Plasma-based Mass Analysers (ICP-MS). Speciation for Proteomics, integrating MS “molecular” (MALDI-TOF and Electrospray-Q-TOF) and “atomic” (ICP-MS) for the elucidation of metalloproteins and some prostrasductional modifications (e.g.glycosylation) related to certain pathological status.Dr Montes-Bayón participates in several national research projects from the Ministry of Education and Science and she is member of the Board of the Mass Spectrometry Group in Spain and has belonged to the National Spectroscopy Group and to the Advisory Board ofJAASsince 2006.Yasumitsu OgraYasumitsu Ograobtained his BS degree from Tokyo University of Pharmacy and Life Sciences in 1991, and his PhD from Chiba University (Japan) in 1996. He continued carrying out scientific research as a post-doctoral fellow at the National Institute of Industrial Health (Japan). Since 1997, he has worked in the Department of Toxicology and Environmental health, Graduate School of Pharmaceutical Sciences, Chiba University as a research associate (1997–2003) and an associate professor (2004–present). He was a visiting scientist of the French National Research Council (CNRS) in 2002 and 2003. His current research interests include the elucidation of mechanisms underlying physiological and toxicological effects of metals and metalloids, and development of a novel approach to metal/metalloid toxicology by combination between speciation/fractionation of metals/metalloids and molecular biological techniques. His special attention is devoted to toxicology in metallomics,i.e., toxicometallomics.Joanna SzpunarJoanna Szpunargraduated from the Warsaw University of Technology and then moved to the University of Warsaw from which she obtained her PhD (1992) and DSc (habilitation) (2000) degrees. She was research fellow at the University of Antwerp (UIA) (1993–1994), and fellow of the European Environmental Research Organization (EERO) at the University of Bordeaux (1995–1996). In 1997 she joined the National Research Council of France (CNRS) as research engineer at the Laboratory of Bioinorganic Analytical and Environmental Chemistry in Pau. Szpunar is titular professor of chemistry (Poland). Her research interests focus on the development of hyphenated techniques for thein vivospeciation and fractionation of trace metals and metalloids in natural products and biological systems.Jeffrey ZaleskiProfessor Jeffrey Zaleskireceived his BS degree from SUNY at Geneseo in 1988 followed by his PhD from Michigan State University in 1993. He was subsequently awarded Jane Coffin Childs Postdoctoral Fellowship at Stanford University where he studied Physical-Bioinorganic chemistry under the direction of Professor Edward I. Solomon. Professor Zaleski joined the faculty at Indiana University in 1996.His research interests are in the fields of metals in medicine, bioinorganic chemistry, synthetic chemistry, and materials. Professor Zaleski and his group have four main thrust areas. (1) Attempting to harness the power of naturally occurring chemical functionalities for applications as metal-mediated biomedical reagents. These systems are primarily based on radical or diradical intermediates whose reactivity is difficult to control and systematically modulate. (2) The development of novel nano-scale architectures as hybrid materials for optics, photo-magnetic switching, and nanomedicine applications. Constructing these materials involves development of new synthetic strategies or novel ligand coatings with specific properties to enable optical or magnetic excitation. (3) Applications of spectroscopy to electronic structure of inorganic and bioinorganic metal sites involved in thermal or photoinduced processes such as energy transfer, or biochemical gene expression. (4) Synthesis of unique porphyrinoid architectures that have unusual optical properties for energy-related photoelectronic or synthetic applications requiring extended chromophores.

ISSN:1756-5901    

DOI:10.1039/b925330k

出版商:RSC    

年代:2009

数据来源: RSC

摘要:

Metallomicspublishes original research and topical reviews, which provide insight into the role of metals in the life sciences. This includes:• Chemical speciation, dynamics and kinetics of trace elements in biological systems• Elemental distributions and concentrations linked to the genome• Regulation of the uptake, accumulation and metabolism of metals and other trace elements in biological systems• Physiological and pathological mechanisms related to trace elements in human health and disease• Structural analysis and coregulation of elements within the metallome, including the survey and identification of metalloproteins/enzymes• Genetic and molecular genetic basis for regulation of metallomes and epigenetic factors relative to the organism• The interaction of metallodrugs, including chemotherapy agents, with biological organisms, including in clinical use• Metal exchange between biota and the environment• Bioimaging and biosensing of metals, including analysis of diagnostic and therapeutic radioactive metals• Certified reference materials for biological applicationsReadership will be cross-disciplinary and include researchers in academia and industry working in analytical science, biogeochemistry, bioinformatics, biological catalysis, biological environmental science, cell biology, clinical chemistry, environmental health, medicine, metallobiochemistry, microbiology, nutritional chemistry, pharmacology, plant biochemistry and physiology and toxicology.

ISSN:1756-5901    

DOI:10.1039/b821668c

出版商:RSC    

年代:2009

数据来源: RSC

摘要:

Welcome to the first issue ofMetallomics: Integrated Biometal Science.The study of metals in biological systems is an increasingly important area of research. Metallomics is a newly emerging scientific field that is receiving great attention as a new frontier in the study of trace elements in the life sciences. It is a global discipline encompassing many areas including biology, chemistry, geology, medicine, physics, and pharmacy.As this field brings together researchers from such diverse areas, we anticipate thatMetallomicswill help to bridge the gap between researchers from different backgrounds so that ideas can be shared and the field progresses to the benefit of all. Our journal will serve as a focus for the community of metallomics researchers to come together and gain new perspectives and insights. It is our aim to reflect the interests of the emerging community and to support you as your community grows.We would like to acknowledge the work of those whose vision has led to the establishment of this field, including Bob Willams (R. J. P. Williams,Coord. Chem. Rev., 2001, 216–217; 583–595), Hiroki Haraguchi (H. Haraguchi,J. Anal. At. Spectrom., 2004,19, 5–14) and Joanna Szpunar (J. Szpunar,Anal. Bioanal. Chem., 2003,378, 55–56), and to all those who continue to contribute to the emergence of metallomics, without whom we would not be launching this exciting new journal.

ISSN:1756-5901    

DOI:10.1039/b820741k

出版商:RSC    

年代:2009

数据来源: RSC

摘要:

Joe Carusoholds a PhD from Michigan State University. After a one-year post-doctoral fellowship at the University of Texas-Austin he joined the UC faculty and, since then, he has authored or co-authored about 360 scientific publications and presented over 300 invited lectures at universities and at scientific meetings. He has more than 7000 citations to research papers and review articles since 1980. Caruso is a member of the American Chemical Society, Society for Applied Spectroscopy and a Fellow of the Royal Society of Chemistry and former editorial board chair ofJAAS.His research interests have long involved atomic mass spectrometry, which led to elemental speciation studies and ultimately metallomics research. Current interests involve using atomic and molecular mass spectrometry to better understand cell toxicity from the molecular point of view contemporaneously with major cell events. His research on metals, non-metals and their differing forms has implications for chemical warfare agent detection, environmental remediation, and health care.He has been honored by Eastern Michigan University with its 1990 Distinguished Alumni Award, by the American Chemical Society with the 1992 Cincinnati Chemist of the Year Award, the Federation of Analytical Chemistry and Spectroscopy Society with the 1994 Anachem Award, and with the 2000 Spectrochemical Analysis Award given by the Analytical Division of the American Chemical Society. Recently he received the University of Cincinnati-Excellence in Doctoral Student Mentoring Award and the UC 2007 Rieveschl Excellence in Research Award.Ariel D. Anbaris a biogeochemist in the Department of Chemistry and Biochemistry and the School of Earth & Space Exploration at Arizona State University. He received an AB in Geological Sciences and Chemistry from Harvard University in 1989, and a PhD in Geochemistry from the California Institute of Technology in 1996.Anbar’s research centers on the chemistry of transition elements in the environment. A major emphasis of this research is to determine how the abundances of bioessential elements such as Fe and Mo changed in the oceans during Earth history. The goal is to understand how these changes affected evolution.This research is pursued through the development and application of novel analytical approaches using ICP mass spectrometry, In particular, Anbar’s group pioneered the use of multiple-collector, magnetic sector ICP-MS to precisely measure mass-dependent variations in the isotopic abundances of transition elements that arise from mass fractionation. This work reveals that abundance variations of 0.01–0.1%/amu, once undetectable, are ubiquitous in nature. Such measurements in natural samples, informed by laboratory experiments and theoretical studies, provide insights into the environmental chemistry of metals and the metal-centered interactions between organisms and their surroundings. Applied to the geologic record, such “metal stable isotope” studies provide information about metal biogeochemical cycles on the ancient Earth, environmental changes that perturbed these cycles, and biological activity in the distant past.Hiroki Haraguchigraduated from the University of Tokyo (1965), from which he also received MSci and PhD degrees in 1967 and 1973, respectively. From 1969, he served as assistant professor at the University of Tokyo, research chemist at the National Institute for Environmental Studies, and associate professor at the University of Tokyo. From 1975 to 1977, he was a post-doctoral fellow at the University of Florida, where he worked with Prof. J. D. Winefordner. He was promoted to full professor of analytical chemistry in the Department of Applied Chemistry at Nagoya University in 1988, and retired in 2007. He is currently Emeritus Professor of Nagoya University as well as the program officer of the Ministry of Environment and an associate member of the Science Council of Japan. His research interests are the development of highly-sensitive analytical methods for chemical speciation of trace elements in biological, environmental and geochemical systems. He proposed “metallomics as integrated biometal science” inJAASin 2004, which has been receiving great attention as a newly emerging scientific field in life science, complementary to genomics and proteomics. Furthermore, he has been attempting to establish the concept of “cell microcosm”, which indicates the existence of all elements in a single biological cell.Gary M. Hieftjeis Distinguished Professor and Mann Chair of Chemistry at Indiana University in Bloomington, Indiana. His research interests include the investigation of basic mechanisms in atomic emission, absorption, fluorescence and mass spectrometric analysis, and the development of instrumentation and techniques for atomic methods of analysis. He is also interested in the on-line computer control of chemical instrumentation and experiments, the use of time-resolved luminescence processes for analysis, the application of information theory to analytical chemistry, analytical mass spectrometry, near-infrared reflectance analysis, metallomics, and the use of stochastic processes to extract basic and kinetic chemical information. He has won numerous awards in the fields of analytical chemistry and spectroscopy, has held major offices in several scientific societies, and has served on the editorial boards of many major journals. He is the author of over 500 publications, 10 books, and 15 patents. More than 65 students have received doctorates under his direction.Ryszard Lobinskiis research director at the CNRS (Laboratory of Analytical Bioinorganic and Environmental Chemistry in Pau) and professor of chemistry at the Warsaw University of Technology. He obtained his PhD (1989) and DSc (habilitation) (1994) degrees from the Warsaw University of Technology, Poland. He held post-doctoral positions at the Institute of Spectroscopy and Applied Spectrometry (ISAS) in Dortmund (1990) and at the University of Antwerp (UIA.) (1991–1994) before joining the National Research Council of France (CNRS) in 1994. R. Lobinski is the author or co-author of over 200 articles in international journals, 3 books, 3 edited journal issues, and about 90 invited lectures at international meetings. He received the CNRS 2006 Silver Medal and is a Fellow of the Royal Society of Chemistry. He is also the co-director of UltraTrace Analyses Aquitaine (UT2A) (a startup company at the University of Pau) and Past-President of the Analytical Chemistry Division of IUPAC. His principal research interest is the development of analytical approaches to species-specific (speciation) analysis for trace and ultratrace metals in environmental and nutrition chemistry and in the life sciences.Thomas V. O’Halloranis Morrison Professor at Northwestern University, Department of Chemistry and Department of Biochemistry, Molecular Biology and Cell Biology. He completed his PhD at Columbia University, New York, with Professor S. J. Lippard and followed this with a post-doctoral position at MIT with Professor C. T. Walsh.Professor O’Halloran’s research interests include the chemistry and biology of transition metal receptors; transcriptional regulation mechanisms; metalloregulatory proteins; molecular and cell biology of copper, iron and zinc; metal ion chaperone proteins for Cu and Zn; mercury thiolate and protein chemistry; high-valent, iron-oxo chemistry; non-heme iron enzymes; design and development of inorganic-based antitumor drugs and protease inhibitors.Professor O’Halloran’s awards and recognitions include: Member, Advisory Board, US DOE Biological Sciences Directorate; NIH MERIT Award (Method to Extend Research in Time) for grant “Mechanistic Studies of the MerR and Fur Metalloregulatory Proteins”; Advisory Board, Center for Environmental Bioinorganic Chemistry Princeton University; Robert Lurie Cancer Center at Northwestern University – Director of Cancer Genes and Molecular Regulation Basic Research Program; Leadership Council; Co-Director of Molecular Oncogenesis Basic Research Group; Fellow, American Association for the Advancement of Science; Fellow, John Simon Guggenheim Memorial Foundation; Leroy Hall Award for Excellence in Teaching given by the Weinberg College of Arts and Sciences; American Society of Biochemistry and Molecular Biology Scientific Achievement Award sponsored by Schering-Plough; Associated Student Government Faculty Honor Roll; Mortar Board-Faculty Honor Roll of Outstanding Teachers; The Camille and Henry Dreyfus Foundation Teacher-Scholar Award; Alfred P. Sloan Research Fellow; Presidential Young Investigator Award, NSF; National Searle Scholars Award, The Chicago Community Trust; The Camille and Henry Dreyfus Foundation Distinguished New Faculty Award; Pegram Award, Columbia University.David E. Saltis a Professor at Purdue University, Department of Horticulture and Landscape Architecture, West Lafayette, Indiana, USA.His long term research interest is to understand the function of the genes and gene networks that regulate the plant ionome (elemental composition), along with the evolutionary forces that shape this regulation. To achieve this his laboratory couples high-throughput elemental profiling, with bioinformatics, genomics and genetics, in both genetic model species (yeast,Arabidopsisand rice) and “wild” plants (Astragalus, Thlaspi and Pteris). He has been involved in such work since his PhD (Liverpool University, UK, 1985–1988) working on the mechanisms of copper tolerance inMimulus gutattus(yellow monkey flower). He also has a BSc in Biochemistry (University of North Wales, Bangor, UK, 1981–1984) and an MSc in Computer Science (Hallam University, UK, 1984–1985). He has published over 75 peer reviewed papers since 1989 with approximately 4000 citations. During his career he has won competitive research funding from USDA, DOE, NSF and NIH. He is a member of the American Society of Plant Biologists and has sat on both the Education and Public Affairs committees. He is also a Monitoring Editor for Plant Physiology, and on the Editorial Boards of the Brazilian Journal of Plant Physiology, BMC Plant Biology and the International Journal of Phytoremediation.Bibudhendra (Amu) Sarkaris a leading authority in inorganic biochemistry. He graduated with a PhD degree in biochemistry from the University of Southern California, Los Angeles in 1964. He joined the Department of Biochemistry, University of Toronto and Research Institute of the Hospital for Sick Children with a Medical Research Council Scholar award. He was a Visiting Scientist at the Institute de Biologie Physico-Chimique, Université de Paris-Sorbonne, France where he worked on theab initiomolecular orbital calculations of metal-binding sites of proteins. He was a Visiting Scholar working on protein structure in the Department of Biochemistry, University of Cambridge, UK with an award from the Nuffield Foundation. As the Head of Structural Biology and Biochemistry Department at the Hospital for Sick Children, he established a major research centre with a broad initiative in protein structure and function. He discovered the copper-histidine treatment of Menkes’ disease, a devastating neurodegenerative disease in children caused by a genetic defect affecting copper transport. His pioneering research on the structure and function of metal-binding proteins led to the discovery of the ATCUN motif, which can serve as a probe for protein structure determination by paramagnetic relaxation enhancement NMR spectroscopy and selective cleavage of DNA. His major research interests are in the area of metal-related genetic diseases with a special emphasis on Wilson’s and Menkes’ diseases and studying the effects of toxic metals in the environment and their impact on human health. Dr Sarkar is an Emeritus Professor at the University of Toronto and the Hospital for Sick Children, Toronto.Hongzhe Sunobtained his PhD from the University of London (with Peter J. Sadler) in 1996. After post-doctoral work at the University of Edinburgh, he joined the Department of Chemistry at the University of Hong Kong in 1998, where he is currently a professor. He was the recipient of the NSFC Outstanding Young Scholar Award in 2005, and serves on the advisory board of theJournal of Biological Inorganic Chemistry. His research interests are centered on metallodrugs and metalloproteins, inorganic structural biology and metallomics.Marco Aurélio Zezzi Arrudawas born in Piracicaba, São Paulo state, Brazil in 1965. In 1996 he assumed the position of Assistant Professor at the University of Campinas-Unicamp, Department of Analytical Chemistry, becoming Associate Professor in 2001. The main research lines of his research group include mass and atomic spectrometry, sample preparation, mechanization and bioanalysis with an emphasis on metallomics. In the field of metallomics his current research interests include interdisciplinary work involving comparative metallomics of plants (i.e.soybean, sunflower) and human body fluids (i.e.blood serum, saliva, urine) to identify possible biomarkers for transgenic species and human diseases, as well as to evaluate reactive oxygen species production, and (metallo)protein responses under metallic stress in a given system.Ivano Bertiniis Professor of Inorganic Chemistry at the University of Florence and is Director of the Magnetic Resonance Center (CERM). He has received several honours, including three Laurea Honoris Causa (from the universities of Stockholm, Ioannina and Siena). He is a member of the Academia Europaea and the Italian Accademia dei Lincei, and is, or has been, on the editorial staff or advisory board of over 20 of the most authoritative journals in chemistry, biochemistry and inorganic chemistry.Since 1975 he has studied the structure–function relationships of metalloproteins through biophysical methods. In 1990, he transformed his lab into an NMR lab for the structural biology of metalloproteins, and eventually pioneered the exploitation of genome data banks. He has pursued advancements in technology for solution structure determination, particularly for paramagnetic metalloproteins, and developed specific software applications. He has also established a molecular biology department for high-throughput protein expression in structural genomics projects on metalloproteins. He has published over 600 research articles and has solved more than 100 protein structures.In 1999 he founded the present Magnetic Resonance Center (CERM) at the University of Florence in an independent and prestigious building hosting an impressive battery of NMR spectrometers. The Center constitutes a major NMR infrastructure in the Life Sciences.Jörg Bettmerfinished his chemistry degree (University of Münster, Germany) in November 1992. Afterwards, he started his PhD in the Department of Analytical Chemistry at the same university under the supervision of Prof. K. Cammann, which he finished in 1996. After several short-term post-doctoral posts he moved to the University of Mainz, Germany, in 2000, where he obtained his habilitation in summer 2004 under the supervision of Professor Klaus G. Heumann. After a visiting professorship for environmental and analytical chemistry at the Humboldt-University in Berlin in 2004/05 he moved back to Mainz, where he was appointed assistant professor in analytical chemistry until 2007. In May 2007 he moved to the University of Oviedo, Spain (Professor Alfredo Sanz-Medel), where he is currently holding a position as “Ramón y Cajal researcher”.His research interests and experience are mainly centred in the field of elemental speciation with special emphasis on the development of hyphenated techniques. This includes labelling strategies for (quantitative) protein and metalloprotein determinations.Zhifang Chaiis a radiochemist working at the Multidisciplinary Initiative Centre, Institute of High Energy Physics, the Chinese Academy of Sciences. He graduated from Fudan University, China, in 1964. As a fellow of the Alexander von Humboldt Foundation, Germany, he worked at Cologne University from 1980 to 1982 in the field of nuclear technology and its applications. Later, he worked in France, the USA, the Netherlands and Japan. He has long been involved in the methodology of nuclear analytical techniques and their multidisciplinary applications, especially in the study of the chemical speciation of trace elements in environmental and biological systems. He has authored or co-authored over 332 papers in peer-reviewed journals, 6 Chinese books and 3 English books. He is a member of many domestic and international scientific societies. In 2005 he was awarded the George von Hevesy Award – the premier international award of excellence to honour outstanding achievements in radioanalytical and nuclear chemistry. In 2007 he was elected as a member of the Chinese Academy of Sciences. His present interest is to develop novel nuclear analytical methods for the study of metallomics.Heidi Goenaga-Infantegraduated from the University of Havana, Cuba and was then awarded a MUTIS fellowship from the Spanish International Cooperation Agency at the University of Oviedo, Spain from which she obtained her PhD in 1999. She was a research assistant at the Micro and Trace Analysis Centre (MiTAC), University of Antwerp from 1999–2003. In 2003, she joined LGC’s mass spectrometry team in Teddington, United Kingdom as a senior research and development scientist.Dr Goenaga-Infante is currently the lead scientist of speciation analysis and metallomics research at LGC. Her research interests concern the combined application of elemental and molecular mass spectrometry with chromatography for heteroatom speciation analysis of dietary substances, supplements and clinical samples and the high accuracy determination of heteroatom-containing species in these substances by IDMS approaches, with the aim of characterising new “speciated” certified reference materials. She has undertaken and managed a wide variety of projects and studies including the coordination of CCQM (Consultative Committee on Amount of Substance) international intercomparisons in the speciation field.Jose Luis Gómez-Arizais full professor of Analytical Chemistry at the University of Huelva. He received his BSc (1973) and PhD (1976) from the University of Sevilla. He was temporary assistant professor (1973–77) and permanent assistant professor (1977–93) at the University of Sevilla. In 1993 he moved to the University of Huelva where he became a full professor. His research interests include the investigation of new analytical approaches for chemical species characterization using hyphenated techniques and the analysis of metal species involved in environmental, food and health issues. The study of metalloproteins and other metal-linking biomolecules using multidimensional analytical approaches (metallomics) is now under his attention considering its integration with other -omics such as proteomics and transcriptomics. He is the author or co-author of over 250 scientific publications, 2 books, and holds several patents. Over 25 students have received doctorates under his direction; many others have received MS degrees, and scores of undergraduates and visiting scientists have performed research in his laboratory.J. L. Gomez-Ariza is a member of the Spanish Society for Analytical Chemistry (SEQA), Royal Society of Chemistry, American Chemical Society and American Society for Biochemistry and Molecular Biology. He was honoured with the Andalusia Research Award in 2003 and the Huelva Industry Excellence Research Award also in 2003. He was president of the Speciation Group of SEQA and the Andalusia Group of the Spanish Society for Analytical Chemistry (GRASEQA). In 2003, he was appointed as an academic of the Academy of Sciences, Arts and Letters of Huelva.Rudi Grimmreceived his PhD in Biology at the University of Munich in 1987. After completing a post-doc at the University of Freiburg/Germany and the Riken Institute in Tokyo, Japan he joined Hewlett-Packard as a senior life science application chemist in 1991. In 1998 he left the company and became the head of protein chemistry at the Munich based proteomics company Toplab. In June 1999 he joined Hexal Pharma to establish biotech laboratories for the development of generic recombinant protein drugs. In September 2002 he rejoined Agilent Technologies as the worldwide proteomics and metabolomics market development manager. Since May 2006 he has been working at the Agilent Technologies headquarter in Santa Clara, California.Norbert Jakubowskihas been a senior scientist working in elemental mass spectrometry at ISAS-Institute of Analytical Sciences for more than 25 years. His research interests are related to trace elemental analysis with a focus on instrumental developments in plasma source mass spectrometry (ICP-MS, GD-MS) and method development for the speciation of hetero-elements in biomolecules (P, S, As, Se, Cr, Ni, Cd, Pt, Pd, Rh). By using ICP-MS he has studied the adduct formation of DNA with alkylating compounds. His team have developed a new laser ablation cell coupled to ICP-MS to investigate metalloproteins after PAGE separation directly in the gel or after electroblotting onto membranes. This approach was used to study the binding of Cd in plant proteins, to detect selenium-containing proteins in fish samples or to quantify the phosphorylation degree of proteins. A few years ago he started to develop labelling techniques for proteins and antibodies by using metal tags. He is now applying this novel technology for the sensitive detection of cytochromes P450 in multiplexed assays.Dr Jakubowski is a member of the editorial board ofJAAS(Reviews Editor) and of the advisory boards of the journals:Analytical and Bioanalytical ChemistryandAnalyst.Bernhard K. Kepplerreceived his diploma and PhD in chemistry from the University of Heidelberg in 1979 and 1981, respectively, and a PhD in medicine from the German Cancer Research Center of Heidelberg in 1986. He habilitated and gained the qualification of a university lecturer in inorganic chemistry at the University of Heidelberg in 1990. In 1995, he joined the Institute of Inorganic Chemistry at the University of Vienna as a full professor. He is Head of the Institute of Inorganic Chemistry and Vicedean of the Faculty of Chemistry at the University of Vienna.David W. Koppenaalis a Laboratory Fellow, Associate Director of the Biological Sciences Division, and Chief Technology Officer of EMSL at Pacific Northwest National Laboratory. Before joining the Advisory Board ofMetallomics, he has been a member of the Editorial Board ofJAASand is currently an Advisory Board member. Dr Koppenaal’s research interests include atomic mass spectrometry instrumentation and applications, with special interests in the metallomic applications of ICP-MS, and collision/reaction cell, high-resolution MS, and new generation MS detection techniques. Dr Koppenaal is a Fellow of the Royal Society of Chemistry and the American Association for the Advancement of Science (AAAS).Peter M. H. Kroneckholds a Diploma in Chemistry from Universität Basel, Switzerland, and completed his Dr rer. nat. studies at Universität Konstanz, Germany. He undertook post-doctoral studies at Utah State University, USA and his habilitation at Universität Konstanz, Germany.He is currently Professor of Biochemistry, Universität Konstanz (since 1986) and his professional experience includes Visiting Scientist, Natl. Biomed. ESR Center, Milwaukee, USA (1988–1994), Head, Bioinorganic Research Group, Universität Konstanz (1980–1986), Visiting Scientist, Weizmann Institute of Science, Rehovot, Israel (1977) and Visiting Scientist, University of Wisconsin Madison, USA (1976).His achievements include: Chair of “Metals in Biology” GRC (2009); Organizer DAAD Summer SchoolMetals in Biology—Key Elements of Life, Cuernavaca, Mexico (2008); Member Natl. Org. Committee, EUROBIC 4, Garmisch-Partenkirchen (2004); Organizer/Chair, Intern. SymposiumCopper 2003, Konstanz (2003); LecturerMetals in Biological Systems, Center for Intern. Studies, Grinnell College, USA (2003); LecturerCu Enzymes and Proteins, Louvain-la-Neuve, Belgium (1985–2003); Medal of the European Society for Bioinorganic Chemistry, received at EUROBIC 6, Lund, Sweden (2002); Organizer/Co-Chair, 13thIntern. ConferenceFlavins and Flavoproteins, Konstanz (1999); Organizer/Co-chair, Intern. Volkswagen-SymposiumElectron Transfer, Konstanz (1999); Natl. Org. Committee of ICBIC 7, Lübeck (September 1995); Organizer/Chair, Intern. SymposiumFeS Proteins, Konstanz (1994); Organizer/Chair, Intern. MeetingRedox Cofactors and Metals in Proteins, Konstanz (1991).Maria Montes Bayónis Associate Professor at the Department of Physical and Analytical Chemistry of Oviedo University since 2007. After completing her chemistry degree at the University of Oviedo (1993), she performed her MSc Studies at the University of Plymouth (UK) under the supervision of Dr Hywel Evans and Prof. Les Ebdon. Once back in Oviedo, she started her PhD studies at Oviedo University under the supervision of Dr J. I. Garcia Alonso and Prof. A. Sanz Medel that concluded in 1999. Her thesis was awarded with a Departamental Prize due to the number and quality of publications obtained. Afterwards, she conducted post-doctoral work as a Fulbright Scholar at the Department of Chemistry in the University of Cincinnati (Ohio, USA) for 26 months, under the supervision of Prof. J. A. Caruso. Back in Spain, she obtained the “Ramón y Cajal” research contract (five years) and she joined the group leaded by Prof. Sanz-Medel. During this period, she obtained a national habilitation and then a permanent position at the Department of Physical and Analytical Chemistry.She is author or co-author of more than 70 scientific publications in international journals and book chapters. This work has also been presented in many lectures at national and international conferences as an invited speaker. Her main research topics include: hybrid techniques for speciation to solve biological and environmental problems. HPLC, Capillary GC and Capillary Electrophoresis, as powerful separation tools are coupled to Plasma-based Mass Analysers (ICP-MS), and Speciation for Proteomics, integrating MS “molecular” (MALDI-TOF and Electrospray-Q-TOF) and “atomic” (ICP-MS) for the elucidation of metalloproteins and some post-transductional modifications (e.g.glycosilation) related to a certain pathological status.Dr Montes-Bayón participates in several national research projects from the Ministry of Education and Science and she is a member of the board of the Mass Spectrometry Group in Spain and has belonged to the National Spectroscopy Group and to the Advisory Board ofJAASsince 2006.Yasumitsu Ograobtained his BS degree from Tokyo University of Pharmacy and Life Sciences in 1991, and his PhD from Chiba University (Japan) in 1996. He continued carrying out scientific research as a post-doctoral fellow at the National Institute of Industrial Health (Japan). Since 1997, he has worked in the Department of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University as a research associate (1997–2003) and an associate professor (2004–present). He was a visiting scientist at the French National Research Council (CNRS) in 2002 and 2003. His current research interests include the elucidation of mechanisms underlying the physiological and toxicological effects of metals and metalloids, and development of a novel approach to metal/metalloid toxicology by the combination of speciation/fractionation of metals/metalloids and molecular biological techniques. His special attention is devoted to toxicology in metallomics,i.e., toxicometallomics.Joanna Szpunargraduated from the Warsaw University of Technology and then moved to the University of Warsaw from which she obtained her PhD (1992) and DSc (habilitation) (2000) degrees. She was a research fellow at the University of Antwerp (UIA) (1993–1994), and fellow of the European Environmental Research Organization (EERO) at the University of Bordeaux (1995–1996). In 1997 she joined the National Research Council of France (CNRS) as research engineer at the Laboratory of Bioinorganic Analytical and Environmental Chemistry in Pau. Szpunar is titular professor of chemistry (Poland). Her research interests focus on the development of hyphenated techniques for thein vivospeciation and fractionation of trace metals and metalloids in natural products and biological systems.Jeffrey Zaleskireceived his BS degree from SUNY at Geneseo in 1988 followed by his PhD from Michigan State University in 1993. He was subsequently awarded a Jane Coffin Childs Post-doctoral Fellowship at Stanford University where he studied Physical-Bioinorganic chemistry under the direction of Professor Edward I. Solomon. Professor Zaleski joined the faculty at Indiana University in 1996.His research interests are in the fields of metals in medicine, bioinorganic chemistry, synthetic chemistry, and materials. Professor Zaleski and his group have four main thrust areas: (1) Attempting to harness the power of naturally occurring chemical functionalities for applications as metal-mediated biomedical reagents. These systems are primarily based on radical or diradical intermediates whose reactivity is difficult to control and systematically modulate. (2) The development of novel nano-scale architectures as hybrid materials for optics, photo-magnetic switching, and nanomedicine applications. Constructing these materials involves development of new synthetic strategies or novel ligand coatings with specific properties to enable optical or magnetic excitation. (3) Applications of spectroscopy to electronic structure of inorganic and bioinorganic metal sites involved in thermal or photoinduced processes such as energy transfer, or biochemical gene expression. (4) Synthesis of unique porphyrinoid architectures that have unusual optical properties for energy-related photoelectronic or synthetic applications requiring extended chromophores.

ISSN:1756-5901    

DOI:10.1039/b821071n

出版商:RSC    

年代:2009

数据来源: RSC

摘要:

1.IntroductionMost of the trace elements in biological systems are bound to biomolecules. Metal-binding compounds play essential roles acting as biological catalysts that regulate reactions and physiological functions in cells and organs. For instance, the metalloproteins which catalyse biological reactions, called metalloenzymes, participate in important biological processes.1Metal-binding biomolecules are also present in degeneration processes: traces of Fe, Cu and Zn are involved in the growth of neurotoxic amyloid fibrils which allow the progression of Alzheimer’s disease.2Furthermore, drugs containing metal atoms are frequently employed in medicine.3Bi complexes have been successfully used therapeutically in antiulcer treatments;4Au compounds are used on the treatment of rheumatoid arthritis,5also exhibitingin vivoantitumor activity when gold is in oxidation state +1 or +3;6and, finally, compounds with Ga,7Ru,8Rh,9Sn10and As11present antitumor properties. Nowadays, the most powerful metallodrugs used are the Pt-containing compounds cisplatin, carboplatin or oxaliplatin, which are applied worldwide in the clinical practice.The antitumor properties of cisplatin (cis-diaminedichloroplatinum(ii)) were discovered in the 60’s by Rosenberget al.12and its clinical use was approved by the FDA in 1978. When DNA reacts with cisplatin, cross-linked adducts are produced, resulting in the distortion of its double-helix structure, with a global bending in the duplex of 35–40° and a local unwinding of 25° in the double helix.13As a consequence of the DNA damage several cellular processes are disrupted, including transcription and replication, being cell death, either by apoptosis or necrosis, finally induced.14As it has been recently summarised, there are four consecutive stages involved in the inhibition of the transcription by Pt-containing drugs: (1) cellular accumulation by both passive and active uptake; (2) activation of the Pt(ii) complex; (3) binding to nucleic acids to form a variety of Pt-DNA adducts, and (4) the cellular response to DNA damage.14Clinical treatments are limited by the toxic side effects such as nephrotoxicity, emetogenesis and neurotoxicity. Nephrotoxicity may be partially inhibited using administration protocols including a previous hydration of the patients with saline solutions15as well as varying the extension of the infusion times, volume and number of the dosages.16Efforts have been made towards the improvement of cisplatin therapeutic properties, looking for minimising its side effects, and as a result, many new Pt-based drugs have been developed, some of them already approved for clinical use or under consideration for approval by regulatory authorities. Carboplatin (cis-diamine(1,1-cyclobutanedicarboxylato)platinum(ii)), which was authorised by the FDA in 1989, is a second generation drug widely used to treat ovarian and lung cancers. Compared to cisplatin, carboplatin presents lower activity and toxicity, can be used more easily in combined therapies and is active against the same type of tumours. Oxaliplatin ((trans-RR-cyclohexane-1,2-diamine)oxalatoplatinum(ii)), a third generation Pt-drug approved by the FDA in 2004, improves the toxic behaviour of cisplatin, does not present cross-resistance with cisplatin and is very effective against colon cancer.17The main side effects of carboplatin and oxaliplatin are myelosupression and neuropathy, respectively.Fig. 1shows some other important Pt-based drugs. Tetraplatin, iproplatin and satraplatin (JM216) are Pt(iv) water soluble compounds, and therefore, suitable for oral administration. Specifically, satraplatin has been extensively studied18and now is under consideration for approval by the FDA for hormone-refractory prostate cancer.19Other promising Pt-drugs are nedaplatin, lobaplatin and picoplatin, the latter in Phase III of clinical trials for small-cell lung cancer.20New generation Pt-based drugs that do not follow the traditional structure–activity rules of platinum cytoxicity established by Cleare and Hoeschele in 197321aretrans-compounds (JM335) or even polynuclear complexes (BBR3464).Structures of the most important Pt-based drugs.The evolution of the number of publications involving cisplatin, oxaliplatin and carboplatin analysis in the last decades shows a great increase. This tendency is due not only to the current interest of the scientific community in Pt-based drugs, but also to the improvement of the analytical tools and methodologies used in such studies. A new discipline, Metallomics,22is being developed to study the evolution of metal or metalloid species and their interaction in time and space with other type of molecules, including biomolecules, focusing both in qualitative and quantitative aspects. Consequently, the goals of metallomics are the determination of the metallome, which is defined as the entirety of metal and metalloid species within a cell or tissue type, and the elucidation of the physiological roles and functions in which any metallo-species may be implicated in a biological system.23Analytical Chemistry methodologies, techniques and instrumentation are essential for the study of metallomes.24The chemical forms and amounts of trace elements present in biological systems are responsible for their bioavailability, toxicity or functionality. For that reason, elemental biospeciation and structural determination studies are essential for the comprehension of the effects produced by metal-containing compounds in living organisms. The high complexity of Pt-based drugs metallomics studies arise from factors like the poor stability of the drugs and drug–biomolecule adducts, their low concentrations in real samples, the different complex sample matrices to be analysed and the high amount of potential biomolecule targets.25,26Pt(ii), as a soft Lewis acid, presents a high affinity for soft bases with donor atoms. It shows a preferential binding toS-donor groups, but also other groups such asN-donors represent significant coordination points, being the affinity to the latter higher than forO-donors. Thus, aside from DNA, peptides and proteins withS- andN-containing aminoacidic residues are potentially reactive molecules towards Pt-drugs.26It has been suggested that on reaction with proteins, cisplatin may bind as a bidentate ligand, for instance, with one of the leaving groups replaced by aS-donor and the other by a neighbouringN- orO- donor. Since the SH → SS bonding is important for the protein conformation, the binding of Pt-drugs to cystines may produce an alteration on the structure and the biological functions of proteins. This fact may be related to the side effects observed during the antitumor treatment.When Pt-based drugs enter the blood stream, a series of interactions with blood components and later on with other molecules on cell membranes and inside the cells take place. The low chloride concentration in cytosols triggers the hydrolysis of cisplatin, producing a series of adducts as a result of the interaction of the evolved drug with cytoplasmic biomolecules. It is believed that some of these adducts are the key to understand the antitumor and toxic effects of Pt-drugs. The extent of the DNA-adducts formation may be limited by the interaction of the drug with cytosolic biomolecules like MT (metallothioneins) or GSH (glutathione), resulting in detoxification or resistance effects. Moreover, the binding of the drugs to blood proteins or the efficacy of the cell uptake could also alter the supply of the drug to the DNA. Other proposed resistance mechanism is related with the capacity of the cell to recognise and repair the damage produced by the drug–DNA adducts avoiding cell death.The low concentration and the high diversity of drug–biomolecule adducts require the use of mass spectrometry analytical techniques, both elemental and molecular, coupled to different separation techniques, for the quantification and structural determination of Pt-adducts.27Depending on the objective, blood, urine, tissues or cell fractions must be analysed, using at least one high resolution separation technique.28Both analysis conditions and sample treatment, such as pre-concentration or clean-up steps, must ensure the preservation of the Pt-species identities. Nevertheless, the difficulty to analyse Pt-adducts at the trace levels present in real samples, preserving their identity along the several steps needed to separate and detect the adducts, has precluded, in general,in vivoexperiments in bio-speciation. Alternatively, many of the reported articles consist inin vitroexperiments that simplify the sample matrix and allow working with more concentrated species. The concordance betweenin vitroandin vivoexperiments is usually a problem because slight differences in the reaction media and analyte concentration results in great differences in the identity and stability of the Pt-species. Results obtained fromin vitroexperiments must be carefully reviewed evaluating their reproducibility in biological living systems, being in any case a previous step to tackle the challenge of analysing real samples.Not only speciation studies are important to evaluate the Pt-based drugs mechanism of action. Accumulation and distribution studies of the drug among different organs, tissues and cell compartments impose the use of cell fractionation techniques and cut-off filters to determine the affinity of the drug and to allow the identification of their main biological targets.29The use of samples from patients with different sort of tumours, ages, administration dosages,etc., forin vivoexperiments, have allowed to draw a distribution map of Pt-based drugs in organisms and are reported in a great number of works.29,30The information collected in this review will help the reader to clarify and sum up the reported data related with the speciation of Pt-containing drugs and the Pt adducts they form with biomolecules and also concerning the determination of total Pt content after the administration of the aforementioned drugs. The most commonly employed analytical tools are presented firstly, including sample preparation, separation and detection techniques. According to the sample matrix, the last section contains a complete summary of the most interesting reported analytical articles.

ISSN:1756-5901    

DOI:10.1039/b911438f

出版商:RSC    

年代:2009

数据来源: RSC

摘要:

Jennifer Meyer got her bachelor’s degree in chemistry and mathematics from Central Michigan University in 2005. She began her doctoral studies in the fall of 2005 and is currently a graduate student in the labs of Dr Dana Spence at Michigan State University. Jennifer’s research has focused on defining new roles for C-peptide and its effect on erythrocytes as it pertains to diabetic complications. Her work has been published in both clinical and chemical journals. After graduation, she hopes to pursue a career in academia.

ISSN:1756-5901    

DOI:10.1039/b817203j

出版商:RSC    

年代:2008

数据来源: RSC

摘要:

Ivano BertiniIvano Bertini is Professor of General and Inorganic Chemistry at the University of Florence and is Director of the Magnetic Resonance Center (CERM). He has received several honors, among which are three Laurea Honoris Causa (from the universities of Stockholm, Ioannina and Siena). He is a member of the Academia Europaea and the Italian Accademia dei Lincei, and is, or has been, on the editorial staff or advisory board of over 20 of the most authoritative chemistry and biochemistry journals. Since 1975 he has studied the structure–function relationships of metalloproteins through biophysical methods. In 1990, he created an NMR lab for structural biology of metalloproteins, and eventually pioneered the exploitation of genome data banks. He has pursued advancements in technology for solution structure determination and developed specific software applications. He has also established a molecular biology department for high-throughput protein expression in structural genomics projects on metalloproteins. He has published over 600 research articles and has solved more than 100 protein structures. In 1999 he founded the CERM in an independent and prestigious building hosting an impressive battery of NMR spectrometers. The Center constitutes a major NMR infrastructure in the Life Sciences.

ISSN:1756-5901    

DOI:10.1039/b912156k

出版商:RSC    

年代:2009

数据来源: RSC

摘要:

Professor Hiroko Kodama is a professor in the Department of Pediatrics at Teikyo University School of Medicine in Tokyo, and part-time instructor in the Department of Developmental Medicine at Tokyo University, Japan. She is also currently Vice President of the International Society for Trace Element Research in Humans.

ISSN:1756-5901    

DOI:10.1039/b816011m

出版商:RSC    

年代:2008

数据来源: RSC