摘要:

The time pattern for the early development of not cell‐bound complement fixing antigen (NCBA) ofM. pneumoniae, Bård strain, in filtrates from broth cultures has been examined. A filter disc technique for the preparation of washed cell inoculates which are free of NCBA and have good cell viability is described. Using this technique, NCBA could be demonstrated significantly earlier than the culmination of the number of CFU/ml, i.e. during the late growth phase of the cultures. No NCBA could be traced during the first days of incubation. The precipitability of NCBA with ammonium sulphate is reported, and a preliminary precipitation technique has been employed in the demonstration of NCBA, together with the direct measurement technique reported earlier (3, 4). The sensitivity of the former method proved to be higher than that of the latter one. It is also reported that growth ofM. pneumoniaecolonies can be initiated from Millipore® 0.22 μ filter discs that have been used for filtration of broth cultures of the organism. It is suggested that the significance of this fact in the isolation ofM. pneumoniaefrom fluid materials should be investig

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04274.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Normal sera from 14 species have been tested for activity against crude protein A. Sera from 6 species produced the protein A line in agar gel, but none of the sera gave the protein B line. Sera from 7 species, including 3 of those precipitating protein A, agglutinated tanned sheep erythrocytes sensitized with crude protein A. With a few individual exceptions, all the sera tested agglutinated Cowan I bacteria. Isolated γG‐globulins showed activities corresponding to their respective sera. The active part of the γG‐globulins in the precipitation reaction with protein A was located to Fc whereas Fab was the active part both in indirect haemagglutination and bacterial agglutination. Rabbit antiserum to Cowan I bacteria and guinea pig antiserum to crude protein A gave results similar to those of normal sera with regard to precipitation of protein A, indirect haemagglutination and bacterial agglutination. In addition both sera precipitated protein B, this ability being located to the Fab region of the γG‐g

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04275.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Conventional mice and SPF‐mice were infected withAscaris suum.The results showed that much more larvae started migration in the latter group. No delay of the larval penetration of the gut wall, as noticed in the conventional mice, was seen in the SPF‐mice. It is concluded that the factors responsible for the delayed passage of larvae through the gut wall both in conventional mice and in immune mice might be similar in nat

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04276.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The already known toxicity of normal chicken allantoic fluid (AF) for the embryo itself, or for other chicken embryos has been analysed and found to be due the content of thromboplastic lipo‐proteins in the AF. The haemorrhagic syndrome caused by i. v. injection of the embryo with AF is suggested as an experimental model for the study of intravascular clotting and the “defibrination” syndrome. The lethality of the haemorrhagic syndrome could be largely prevented not only by heparin but also by natural inhibitors present in both chicken serum and a variety of mammalian sera. At least one of these inhibitors has been isolated from mammalian sera and identified as the previously described antithrombin III (progressive antithro

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04277.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04278.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY