摘要:

Employing a system for continued pH adjustment and a growth medium containing factors essential for streptococcal growth and SI O production, the formation of SI O at different growth phases was studied. The SI O formed during the early phase of exponential growth could not be completely neutralized by its corresponding antibody thus indicating a lack of or a lower antigenicity of this streptolysin. The mechanism for SI O formation is probably an active process where a feed back system might be involved.

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00036.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Polymorphonuclear (PMN) leukocyte accumulation in different sections of the respiratory tract of mice is a prominent sign after inoculation with parainfluenza 1 virus. Spontaneous invasion of bacteria to the lung, normally present in the upper respiratory tract, was frequently observed from the 2. day after viral inoculation. Penicillin‐G, administered in drinking water during the entire course of experiment eliminated the bacteria from the lungs. The extent of PMN leukocytosis was also reduced in the lungs and to a lesser extent in the circulating blood. The same treatment also reduced significantly the mortality which normally follows the virus infection. It is reasonable to believe that a bacterial invasion, secondary to the effect of primary virus inoculation, is responsible to some extent for the leukocyte accumulation in the respiratory organs, and it may participate in the infectious diseas

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00037.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

A human salivary glycoprotein which exhibits blood‐group substance activity in the ABO and Lewis systems, and which also inhibits the haemagglutinins of influenza virus (Rølla&Jonsen1968) has been analysed. Glycoprotein preparations from a non‐secretor (O, Le a+b‐) and from a secretor (O, Le a‐b+) showed very similar amino acid composition, indicating identical protein cores in the two glycoproteins, whereas the secretor preparation contained more amino sugars and fucose. The salivary blood‐group active glycoproteins had an amino acid composition different from the ovarian cyst blood‐group substances and contained more protein than these

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00038.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Flavobacterium meningosepticumhas been shown to be the cause of hospital infections in the form of meningitis in premature infants. This study reports the result of a search for this micro‐organism among specimens submitted for examination forNeisseria gonorrhoeae.Out of 27,600 specimens examined, 88 strains ofF. meningosepticumwere isolated, the majority originating from females. There were no characteristic clinical findings and no specific symptoms in the patients. It is concluded that the female genitals must be considered as possible source of hospital infection withF. meningosepticu

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00039.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

M. pneumoniaecell suspensions prepared by 4 different techniques were examined with regard to the degree of contamination with broth medium constituents, using quantitative protein measurements and gel diffusion tests with antisera against broth medium constituents. Unwashed pellets from high‐speed centrifugation of broth cultures were found to be highly impure. Washing the pellets by repeated resuspensions and centrifugations resulted in an only partial purification, broth medium constituents precipitated as a result of the centrifugation being largely unremovable by this procedure. Prefiltration of the broth medium yielded less contaminated, but still impure, pellet antigens. Suspensions prepared by scraping off washed layers ofM. pneumoniaegrown on a plastic surface did not show gel precipitation reactions of broth medium constituents; their protein figures were relatively high. The significance of this finding is discussed. The yields from cultures on plastic surfaces were found to be greatly increased by a renewa of the broth medium during the incubation period. A filter disc method for harvesting. pneumoniaecells from broth cultures is described, the cells being washed upon, and thereafter scraped off from, the surface of Millipore filter discs type GS (0.22 μ). This method yielded cell antigens of significantly higher purity than the centrifugation method. The filter disc method, in combination with prefiltration of the broth medium, is easily practicable for harvesting washed cell suspensions of broth‐grownM. pneumoniaefor experimental studies and the production of diagnostic anti

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00040.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Rabbits and pigs, artificially infected with washed cells ofCorynebacterium pyogenes, were shown to produce inhibitory antibodies against the bacterial proteinase, as tested by electrophoresis of the sera, combined with the casein precipitating inhibition test (electrophoretic CPI‐test). The antibodies were usually detectable about 14 days after inoculation. Sera of animals not inoculated with microorganisms were also examined. In sera collected from 10 randomly selected animals of each of the species man, horse, chicken, rabbit, and dog, antibodies againstCorynebacterium pyogenesproteinase could not be demonstrated. On the other hand, six, four, and two sera, out of ten, from cattle, pig, and sheep respectively, were found to contain such antiproteinases. In the sera from an additional 25 selected pigs, antibodies were demonstrated in 16; most of these pigs were shown to have chronic corynebacterial infection. Antibodies againstCorynebacterium pyogenesproteinase were demonstrated in most of the sera from 34 selected cattle of different ages, especially from the older ones. In some of these animals the bacterium was isolated from pyogenic processes; in other animals there were strong indications of a corynebacterial infection, but in other animals there was no direct indication of such an infection, although previously experienced infections, with or without clinical manifestations are also supposed to have occurred in these cases. The diagnostic value of qualitative or quantitative determination of such antiproteinases in cattle and pig is considered to be of limited value, due to the frequent occurrence of such antibodie

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00041.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00042.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00043.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1971.tb00044.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY