摘要:

117 patients with acute or chronic hepatitis, 190 patients with liver cirrhosis, 50 patients with hepatitis following mononucleosis infectiosa, and 114 healthy blood donors were examined for the Australia antigen or hepatitis‐associated antigen demonstrated byBlumberg et al. in 1965. The Au‐antigen was demonstrated in 45 out of 106 patients with acute viral hepatitis, in 3 out of 6 patients with chronic hepatitis, and in 10 out of 190 patients with liver cirrhosis. Au‐antigen was demonstrated in 27 per cent of patients with serum hepatitis. In sera examined within the first two weeks following the onset of the disease, Au‐antigen could be demonstrated in 82 per cent of the cases of SH and in 37 per cent of those of IH. In 29 out of 45 Au‐antigen‐positive patients, a conversion from Au(+) to Au(‐) was found during the period of observation and the Au‐reaction became negative in the majority of patients 4–5 weeks after the commencement of the disease. The Au‐antibody was only demonstrated in one patient (21 weeks after the on

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04354.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The effect of trimethoprim alone and in combination with sulphamethoxazole was investigated on mice experimentally infected withToxoplasma gondii. The treatment was given per os. No effect was achieved with trimethoprim alone. Trimethoprim had, however, a significant sulphonamide potentiating effect on the survival‐time of mice intraperitoneally infected withT. gondii. The carrier rate after the treatments were concluded was higher among sulphonamide treated animals than among animals treated with both sulphonamide and trimethoprim, but the difference was not significan

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04355.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Centrifugation of frozen mouse peritoneal exudate gave a concentrated solution of the bulk of the solute at the bottom of the centrifuge tube. The Toxotoxin present in the exudate remained embedded in the ice above, a fact which was utilized as a means for its isolation by a method called “ice filtration”. Toxotoxin was thus obtained as a large molecular entity which had the same lethal activity on mice as that of the exudate from which it was isolated. It was shown that Toxotoxin was formed by the aggregation of smaller, inactive molecules. The lethality of Toxotoxin was, therefore, considered related to its molecular weight. In view of these facts it would probably be more appropriate to call Toxotoxin a particle rather than a molec

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04356.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The reactivity between protein A ofS. aureusand serum globulins from various species was found to vary. Sera from three species (man, dog and swine) gave high titre for non‐specific staining in the fluorescent antibody test (FAT), formed distinct precipitation lines with protein A and absorption with Cowan type I removed all γG‐globulin components to such a degree that only traces were left. Sera from two other species (cow and sheep) showed a less marked or weak reaction in FAT, did not readily precipitate protein A and by absorption with Cowan type I the slow γG‐globulin component was completely removed, whereas the fast γG‐globulin seemed to be

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04357.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Attempts to isolate thymine, thymidine or TMP deficient mutants fromNeisseria meningitidiswere altogether negative. This led to the examination of the growth response and the uptake of these compounds when exogenously added. The growth rate was slightly increased upon their addition to cultures of meningococci adapted to growth on basal media. At the same time, radioactivity was taken up from labelled thymine to an extent of 5.6 per cent of the material added, from thymidine to an extent of 0.6 per cent, and from TMP to an extent of 0.05 per cent. The addition of purine deoxyribonucleosides or the base analogue 5‐fluorodeoxyuridine did not increase the uptake of labelled material from thymidine. Growth inhibition which was observed upon the addition of deoxyadenosine, 5‐fluorodeoxyuridine or 5‐fluorouracil has been disc

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04358.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

During an epidemic of hepatitis at a dialysis unit blood samples were regularly obtained during an 18 month period from staff members and patients and examined for increase in GPT and for the occurrence of Au‐antigen. During this period hepatitis was diagnosed in 30 members of the staff and in 30 patients. The clinical picture in the former group differed essentially from that in the latter. The disease was more severe but shorter in the members of the staff than in the patients receiving haemodialysis. The 2 groups differed from one another also in respect of the occurrence of Au‐antigen in the serum. In the staff the Au‐antigen appeared in association with the clinical signs of hepatitis and usually disappeared within 12 days after the onset of the disease and long before the clinical signs. In the patients with renal disease, however, the antigen appeared in the serum much earlier, usually 5 weeks before other signs of hepatitis. Once the patients had become positive they usually remained so for long periods of time. Blood used for dialysis should be examined for Au‐antigen to reduce the possibility of the blood being a portal of entry of infectious hepatitis at a department of dialysis. Patients undergoing dialysis should be regularly examined for the presence of Au‐antigen in

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04359.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Under mild conditions, saline extraction of tubercle bacilli produced an immunogenic mixture which, in rabbits, gave rise to precipitating sera with high fluorescence antibody (FA) titre. Some purification of the major antigens was achieved by means of ion‐exchange chromatography and disc electrophoresis in polyacrylamide. At least two immunogenic substances were found, each with a different type of immunospecificity. The one gave sera that stainedM. tuberculosisand BCG exclusively and, by means of enzymatic digestion, this was shown to be of protein natur

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04360.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

A high correlation between the results of serotyping for HL‐A determined antigens and reactivity in mixed lymphocyte culture (MLC) was demonstrated. In 42 one‐way MLC's between serotypically identical siblings no stimulation was found; the mean ratio between mixtures and unstimulated controls being 1.13 × 0.44 (s.d.), while the lowest ratio in mixtures between family members differing for one or two HL‐A haplotypes was 3.70. The possibility of an additive effect of non HL‐A antigens on the stimulation provoked by HL‐A antigens was investigated by comparing the variation obtained in stimulation of cells from a given individual by cells from two HL‐A identical siblings with the technical variation of the method. It was concluded that if non HL‐A antigens show an additive effect in MLC this effect is so small that it is undetectable by the present technique. This conclusion was supported by the finding of equal mean stimulation in 24 one‐way cultures between siblings differing for two HL‐A haplotypes and 40 one‐way cultures betwe

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04361.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Currently used serological methods permit the definition of the two HL‐A subloci called “LA” and “Four”. Ten pairs of unrelated cell donors typing identical for four different well defined HL‐A antigens, i.e. two “LA” and two “Four” antigens, were tested in mixed lymphocyte culture (MLC). In all cases unequivocal stimulation was seen indicating that none of these pairs were truly HL‐A identical. This finding may be explained by heterogeneity of the seemingly well defined HL‐A antigens or by the existence of additional subloci of the HL‐A system not recognized by the serological methods. The MLC stimulation provoked by the unspecified HL‐A antigens was compared with results of MLC between siblings being different with respect t

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04362.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The concentrations of IgG, IgA and IgM were determined in serum specimens collected from women with acute salpingitis, from women with infections confined to the lower genital tract and from healthy women. Thirty‐four per cent of the acute salpingitis patients had a marked increase of the serum level of IgM, but normal or only slightly increased serum concentrations of IgG and IgA.Mycoplasma hominiswas recovered from all except two of the salpingitis patients with increased levels of IgM. Indirect haemagglutinating antibody toM. hominiswas found in all but one of these cases. In those salpingitis cases that had serum levels of IgM within the normal range, antibody toM. hominiswas found significantly less ofte

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04363.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY