摘要:

The selection procedure for a new bacteriophage typing set forPseudomonas aeruginosais described. The lytic spectra of 113 internationally available phages derived mostly from previous typing sets, had been grouped by numerical allocating techniques applying the unweighted pair group average cluster analysis to the Jaccard‐Sneath similarity matrix and the matrix for the transformed Yule correlation coefficient. This had rendered dendrograms demonstrating the similarity existing between the lytic spectra of the various phages. From each of the clusters arising above a phenon line drawn at a level with approximately 25 clusters, one representative phage was selected. Accordingly, a series of alternative sets were constructed and analysed for their best conformity to the criteria: (a) lysis of the largest possible percentage of bacterial strains, (b) good differentiation, and (c) convenient number of reactions per plate. On this basis anew primary phage typing setforP. aeruginosaof 19 phages and anauxiliary phage typing setof 5 phages were selecte

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00148.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

A new phage typing set forPseudomonas aeruginosahas been established on the basis of all internationally available typing sets and a few additional phages. The present paper describes some of the important characteristics of the new phage typing set compared to previous sets. In terms of the relevant parameters, the new phage typing set appears to constitute an improvements compared to previous alternatives.

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00149.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The transformations of chenodeoxycholic and lithocholic acid–24–14C were studied in anaerobic cultures from dilution steps of intestinal content from the small intestine, cecum and feces from rats. The distribution of metabolites between supernatant and sediment after centrifugation of the cultures was determined. Lithocholic acid and its main metabolites, 3β‐hydroxy‐5β‐cholanoic acid and 3‐keto‐5β‐cholanoic acid, were partly adsorbed to the microorganisms in the cultures. In contrast, chenodeoxycholic acid and 3α‐hydroxy‐7‐keto‐5β‐cholanoic acid were not found to be adsorbe

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00150.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

An antigen‐free medium, AFH medium, for cultivation ofH. influenzaeis described. The medium is prepared from trypticase‐yeast autolysate, filtrated through a Sephadex G‐25 column. For the medium base only the fractions containing low molecular material were utilized. Addition of different amounts of salt and buffer was tested and the effect of the initial pH of the medium was studied. The amount of glucose consumed during growth was measured with a glucose oxidase test. The AFH medium provided luxuriant growth of all tested strains. The bacterial yield in the fluid medium was 1 times 1010‐1 times 1011per ml and overnight cultures on the solid medium resulted in colonies 1–2 mm in

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00151.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Kidney‐, heart‐ and skin‐grafting were performed from individual donor rats belonging to segregating F2 hybrid populations to recipients of one of the parental strains in two combinations: (AS × BN) F2 → AS and (Fischer × BN) F2 → Fischer. Before grafting all donors were tested for presence or absence of the Ag‐B allele from BN by serotyping and by mixed lymphocyte culture reaction. According to the one locus hypothesis one would expect that organs from F2 donors, which carried the BN allele would be rejected acutely, whereas organs from negative F2 donors would survive for a long time concurrently with acute or subacute rejection of skin grafts. In both combinations, acute or subacute rejection of the skin grafts occurred, whether or not the donor carried the foreign Ag‐B allele from BN, and acute or subacute rejection occurred of all kidneys and hearts from BN positive donors except for a single kidney which survived for 197 days. A very distinctly prolonged survival for both kidney and heart grafts was noticed in both combinations when the donor lacked the Ag‐B allele from BN. However, one exception occurred, viz. a grafted heart which was rejected after 62 days. But in general the prediction came true, and it may be concluded that rejection of kidney and heart grafts is the common rule, when incompatibility at the Ag‐B locus exists, while compatibility at this locus leads to permanent acceptance of organ grafts independent of incompatibilities

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00152.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

By immunochemical quantitation it has been shown that IgA is the main immunoglobulin in bovine lachrymal fluid, constituting approximately 40 per cent of the total protein content. The concentrations of IgM and IgG‐1 were considerably lower. IgG‐2 usually occurred in concentrations too low to be quantitated. While lachrymal IgA levels varied mainly independently from the IgA serum levels there was a significant positive correlation between the lachrymal and serum levels of both IgM and IgG‐1, indicating that the bulk of these immunoglobulins may be derived from the p

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00153.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Methicillin resistance inS. albusstrains isolated from clinical sources was of two types. One type was heterogeneous and highly resistant, the other, non‐heterogeneous and less resistant. Of 80 methicillin resistant strains ofS. aureus, 82.5 per cent were enterotoxin B procedures, but none of the methicillin resistant strains ofS. albusproduced enterotoxin B. Experiments to determine whether any linkage existed between enterotoxin B, nuclease and coagulase production showed that all three characters could vary independentl

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00154.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

When synthesis of vaccinia DNA was inhibited, no haemagglutinin (HA) was produced. The antiviral compound isatin‐β‐thiosemicarbazone (IBT) also arrested the formation of vaccinia HA. It is therefore concluded that the synthesis of vaccinia HA depends on late protein synthesis. We have confirmed earlier findings that 5‐bromodeoxyuridine (BUdR) does not inhibit HA production in doses which completely arrest production of infectious virus. In contrast to earlier findings, mitomycin C inhibited HA production in our experiments. The new antiviral compound rifampicin had no or little effect upon vaccinia HA prod

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00155.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The reactivity of kaolin with different antibodies has been examined. Several antibodies against bacterial, protozoal and viral agents, as well as cold agglutinins, were not removed by kaolin treatment of sera, while mononucleosis antibodies, isoagglutinins, human antibodies to rabbit red cells and rheumatoid factor were almost completely removed by this treatment. The difference in reactivity among these antibodies must be due to an unknown heterogeneity in their physico‐chemical propertie

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00156.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Forty strains of micrococci representing all the 8 biochemical types ofBaird‐Parker(4) were examined for their content of bacterial agglutinogens and antigens sensitizing normal and tanned sheep erythrocytes. Special attention was paid to the urinary pathogens of type 3. Agglutination of the strains in 10 rabbit immune sera against all biochemical types, before and after absorption, showed that theMicrococcusstrains have a number of agglutinogens without any systematic pattern. The majority of the agglutinogens are shared by many strains, irrespective of biochemical type. Agglutinogens characteristic of the biochemical types were not demonstrated. Strains of the same biochemical type were, as a rule, not more related antigenically than strains of different types. The indirect haemagglutinations revealed that the micrococci have several shared antigens sensitizing normal and tanned sheep erythrocytes to agglutination inMicrococcusandStaphylococcus aureusimmune ser

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1972.tb00157.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY