摘要:

By means of double diffusion‐in‐gel, immunoelectrophoresis and crossed immunoelectrophoresis someProteusstrains were examined. In the strain O3:H1 the O3, H1 and a protein antigen common to many members ofEnterbacteriaceaewere identified. An additional 30–40 antigens were noted. No K antigen could be discerned. Forty‐nineProteusstrains of eleven different O groups isolated from patients showed a very similar electrophoretic mobility of the O antigens as compared to the O antigens ofProteusstandard

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02255.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Piglets from 0–50 days of age were exposed to infectiveAscaris suumeggs at dose levels varying from 50–10,000 eggs. A clear inverse relationship was found between the inoculated egg doses and the numbers of worms found in the intestine. Thus, piglets given doses of 1,000 5,000 or 10,000A. suumeggs practically never acquired the infection, the mean percentage of worms established being 0.013 per cent of the administered egg dose. In contrast, when 500 eggs were inoculated, 1.4–5.4 per cent (mean: 2.9 per cent) of the egg dose was established, and with a dose of 50 eggs, 38–100 per cent (mean: 64 per cent) of the inoculated eggs completed their migration and established an intestinal worm population. Newborn piglets inoculated before they had suckled colostrum inhibited the infection as effectively as did older pigs. On the other hand, neonatal piglets did not show the distinct eosinophilia that developed at day 10 in older piglets receiving more than 500 eggs. The findings are dicussed with particular emphasis on the mechanisms involved in the massive elimination of migratingA. suumlarvae following inoculation of large egg doses. Also, the epidemiological consequences of the reported findings are me

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02256.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Purified egg‐grown influenza virus A/PR/8/34 (HON1) was shown to contain the Forssman‐hapten in the lipid fraction. Forssman determinants could not be demonstrated in the influenza proteins. The strain X‐31 (H3N2) was also shown to have the Forssman hapten. The keratosulphatelike host antigen seemed to be located on both types of s

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02257.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Leptospirastrain Pomona was studied by negative staining and ultrathin sectioning techniques. The effect of various fixatives on the preservation of cellular details was evaluated. The helical cytoplasmic cell bodies were surrounded by a triple‐layered cytoplasmic membrane with an intermediate layer in close apposition to the outer aspect of the membrane. The outer envelope of the cell consisted of an ordinary triple‐layered membrane with an additional layer exterior to it. This exterior layer may roll off or break off from damaged leptospira cells, and may then be found as finely striated tubules in negatively stained preparations. Usually cells had two axial filaments, one inserted subterminally at either end of the cell. The filaments were entwined around the cytoplasmic body and were situated between the cell body and the outer envelope. An overlap of the axial filaments on the body of the cell was not observed. Dividing cells had four axial filaments, one inserted at either end of the two daughter cells. The dimensions and the substructure of liberated axial filaments were studied and compared with those on flagella of treponemes and bacte

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02258.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Based on electron‐microscopical studies of an avian conjunctivitis agent propagated in cell cultures and fixed in situ, the agent has been found to be a mycoplasma‐like organism, morphologically and ultrastructurally resemblingMycoplasma gallisepticum.Previously described caracteristica of the agent do not exclude that the agent taxonomically is placed in the mycoplasma group, but additional investigations are needed for a correct plac

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02259.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

One hundred and sixty‐nine strains ofProteusandProvidenciawere studied. A simplified identification procedure in two steps was applied. In the first step, strains belonging to theProteus‐Providenciagroup were identified on the basis of one‐hour tests for phenylalanine deaminase and β‐D‐galactosidase activity. In the second step, differentiation between the fourProteusspecies andProvidenciawas based on H2S production, urease activity, fermentation of maltose, and ornithine decarboxylase activity (HUMO). Identification based on the HUMO‐scheme accorded well with the final identification based on 19 characters. The HUMO‐scheme is therefore recommended for use in routine d

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02260.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

A survey of the nephrotoxic mycotoxins ochratoxin and citrinin in cereals used as feed for bacon pigs was conducted, employing thin‐layer chromatography techniques, in districts of Denmark where the incidence rate of mycotoxic porcine nephropathy was high. A total of 58 per cent of the samples contained ochratoxin A and 9 per cent contained citrinin, always together with ochratoxin A. The detection of the two mycotoxins was confirmed by derivative formation and, for selected samples, by nuclear magnetic resonance spectroscopy (NMR). The maximal concentration of ochratoxin A and citrinin was 27.5 ppm and 2 ppm, respectively. About half of the contaminated samples contained more than 200 ppb, which is the level at which development of porcine nephropathy is possible. This is the first report which elucidates the association of ochratoxin A and citrinin and a naturally occurring disease in domestic animal

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02261.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

A passive agglutination technique has been evolved, using a suspension of balsam of Tolu particles as carrier for protein antigen in saline. The effect of altering the variable factors in the test system is described.

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02262.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

L‐phase variants ofE. colishow a great strain variability as regards their capacity to grow on agar surfaces. Using two different media containing 1000 IU penicillin/ml, L‐phase induction was attempted with 61E. colistrains of different serological types. The use of 10,000 and 50,000 IU penicillin/ml was less favourable. Forty‐seven strains were subcultured. With 32 strains, no or a single subcultivation was possible. With 9 strains, abundant surface growth of L‐colonies was obtained after four subcultivations or more. With three strains, more than 90 serial transfers were performed on penicillin‐f

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02263.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

During 6 months a trial was run on the effect of the addition of 10 per cent sucrose to blood culture media. The standard procedure for blood culturing is to inoculate the sample into 12 individual tubes, and the trial was performed by adding 10 per cent sucrose to half of the tubes and then comparing the results obtained in the tubes with and without sucrose. The overall isolation rate of microorganisms was not affected but the detailed comparison showed that for nearly all species the sucrose containing media tended to yield growth more frequently than the media without sucrose. This difference was statistically significant for enterobacteria andStaphylococcus aureus.

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1973.tb02264.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY