摘要:

This paper includes lectures given in February 1970 at Rikshospitalet, Oslo; The Karolinska Institutet, Stockholm; The Institute for Medical Microbiology, University of Lund; and at The Danish Society of Pathology, Copenhagen.

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04338.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

When rabbits were repeatedly injected with freshly prepared homologous heart homogenate, the anti‐heart antibody formation was weak, whereas the chronic tubulo‐interstitial nephritis simultaneously observed had been very severe. Denaturing of the homogenate by repeated freezing and thawing, however, resulted in a markedly stronger antibody production, whereas, on the other hand, the kidney disease had then been relatively m

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04339.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The blood group and gene frequencies within the ABO, MNS, P, Rhesus, Lutheran, Kell, Lewis and Duffy systems have been determined in samples from 802 Danes aged 50 years and 427 Danes aged 70 years, living in a suburban area of Copenhagen. An antibody screening test was performed on sera from the same persons. When the results of the blood groupings in the two materials were compared, no significant difference could be demonstrated. When the present materials were compared with those reported earlier for Danish populations, no significant difference was observed. The frequency of irregular antibodies against erythrocytes in the two materials was lower than that in materials of patients in Rigshospitalet, but the difference was not significant.

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04340.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

An increase in the immune reaction towards sheep red cells (SRC) was observed in SRC‐stimulated cultures containing leucocytes from SRC‐immunized rabbits when the leucocyte‐SRC suspensions were centrifuged at high speed before incubation. Immune reaction was also evident in a majority of culture tubes containing purified lymphocytes stimulated with SRC and centrifuged at high speed, while no response was observed in non‐centrifuged tubes. The findings indicate that SRC antigen may stimulate lymphocytes directly without the presence of phagocytes if a close contact is obtained between antigen and reactin

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04341.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Pulse‐RNA‐3H extracted from 4 species of moraxellae and neisseriae were hybridized to DNA from 14 species of rodshaped and coccal organisms belonging to these groups, one species ofAcinetobacterand fromEscherichia coli, in 44 out of 64 possible combinations. The affinities observed ranged from zero to 34.2 per cent of autologous hybridization activity (DNA and RNA from the same strain). In the combinations of strains studied, the hybridization affinities were essentially of the same relative order as in transformation of streptomycin resistance. Additional evidence was provided in favour of a recent redefinition of the generaMoraxellaandNeisseria, in which each genus contains both rodshaped and coccal organi

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04342.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

A method for the direct detection of proteinases after electrophoresis in agar gel is described. The demonstration of the zones of enzyme activity was performed by the sensitive Casein Precipitating reaction (CP‐reaction), and from one to three proteinase fractions were demonstrated in the mixtures examined. In addition to the direct application as a separation procedure and as a method for determining migration rates and isoelectric points, the application of this method combined with the serological principle of the Casein Precipitation Inhibition test (CPI‐test) was of particular interest. The method was used with a view to examining the number of proteinases produced by the following species:Aeromonas lique‐faciens, Aeromonas salmonicida, Pseudomonas aeruginosa, Bacillus cereusandCorynebac‐terium pyogenes.The electrophoretical patterns were also studied for trypsin and α‐chymotrypsin. The smallest amount of trypsin which could be demonstrated was of the order

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04343.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The immunoglobulin classes of thein vitroactive lymphocytotoxic antibodies of different antilymphocyte sera have been investigated. Rabbits were immunized intravenously with different numbers of rat spleen cells, biweekly for 1–4 weeks. The effect of the antigen dose and the length of the immunization course was investigated. Lymphocytotoxic antibodies were found both in the IgG and in the IgM fraction of the antisera. The relative contribution of the cytotoxic activity of the IgG and the IgM fraction towards the final cytotoxic activity of the crude antiserum depended on the antigen dose and the length of the immunization course. Rabbits immunized with the antigen mixed with Freund's adjuvant intramuscularly and given a booster dose intravenously showed the same pattern as rabbits immunized intravenousl

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04344.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The gel precipitation reactions between measles antigens and serum specimens of 49 patients with multiple sclerosis and 49 age‐matched controls were studied. At least one gel precipitation line was obtained in 76 per cent of the control sera and in 94 per cent of the patient sera (p<0.01). Five patient sera produced four or five strong precipitation lines against measles antigens, but no lines against the noninfected control antigens. In the serum specimens of the control group not more than three faint precipitation lines against measles antigens were detected. Comparison of the gel precipitation reactions between the sera of these five patients with multiple sclerosis and of a patient with subacute sclerosing panencephalitis indicated an almost identical gel precipitation patter

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04345.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Re‐examination and re‐evaluation of three representativeEscherichia coliK antigens of the L variety have been carried out (K12, K51 and K52). It is concluded that the K antigen (L) as hitherto described is not uniform, since more K antigen determinants exist on the surface. Some of these are truly thermolabile, probably proteins, while others are thermostable and are eluted into the suspension medium during heating. This elution is rarely complete. The complex character of the K antigens cannot be detected in the traditional bacterial agglutination technique, but can be demonstrated in double diffusion in gel and immuno‐electrophoresis and by passive haemagglutination. The implications for K antigen typing are disc

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04346.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

By use of the casein precipitating inhibition test (CPI‐test), sera from various species were found to contain inhibitors against trypsin, α‐chymotrypsin, a number of microbial proteinases, ficin and papain. A semiquantitative measure for the inhibitory capacity of the different sera upon the various enzymes is given. By combination of paper electrophoresis of the sera and the CPI‐test (electrophoretic CPI‐test) it was found that sera from most of the species tested contained two different inhibitors against trypsin, α‐chymotrypsin and proteinases fromBacillus subtilisandAspergillus oryzae. In the sera from man, pig, cattle, sheep, goat, and horse only one inhibitor could usually be demonstrated against the other bacterial proteinases tested. In sera from rabbit, dog, and hen usually two or more, inhibitors could be demonstrated also against these enzymes. The proteinases were inhibited linearly according to the amount of serum added up to 70 per cent inhibition or more. The inhibiting capacity of the different sera for trypsin, α‐chymotrypsin, and

ISSN:0365-5563    

DOI:10.1111/j.1699-0463.1970.tb04347.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY