ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

摘要:

Summary:In the present study we examined the effects of a tumor‐associated fetal antigen (TAFA‐II) on the activity of natural killer (NK) cells isolated from human cancer patients. TAFA‐II suppressed the NK cell response of some patients, and the level of suppression appeared to be independent of tumor type or stage of cancer therapy. No significant correlations were found between lymphocyte, neutrophil, monocyte, or eosinophil populations and TAFA‐induced suppression of NK cell activity. TAFA‐II effects were also not attributable to Ia+cells or to OKT3, OKT4, or OKT8 positive cells. This work confirmed results obtained in the rat model, in which suppression appeared to be directly mediated on the NK cell.

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

摘要:

Summary:An epidemic form of Kaposi's sarcoma associated with the acquired immune deficiency syndrome has been recently described. Seven homosexual men with biopsy‐documented epidemic Kaposi's sarcoma were treated with a human interferon‐&ggr; preparation. All patients had generalized disease. Only one patient had received prior chemotherapy, and one other patient had recovered from a prior opportunistic infection. Interferon‐&ggr; was administered in a dose of 500,000 U intramuscularly daily, with two 10‐day induction courses, separated by a 2‐week medication‐free period. This was followed by maintenance therapy in the same dose twice weekly. Toxicities consisted of a flu‐like illness with high fevers, shaking chills, myalgias, and arthralgias. There were no complete or partial responses. All patients exhibited disease progression, with a rapid progression of previously stable disease necessitating discontinuation of therapy in three patients. We conclude that low doses of this human interferon‐&ggr; preparation are ineffective in epidemic Kaposi's sarcoma.

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

摘要:

Summary:Thymosin fraction 5 (TF5) enhances interleukin‐2 (IL‐2) production by human peripheral blood mononuclear leukocytes (PBL) when cocultured in the presence of phytohemagglutinin (PHA). This important biological activity of TF5 is not due to the presence of two previously well‐characterized component peptides, thymosin &agr;1and thymosin &bgr;4, but rather to a new, still to be identified component of TF5. Comparison of TF5 with other well‐defined thymic preparations and peptides indicates that this biological activity is unique to TF5 and a closely related extract prepared from porcine thymus tissue. Using a standard human PBL cell population obtained by leukopheresis and cryopreservation, it should now be possible to establish a reproducible bioassay for the isolation and characterization of one or more components of TF5 with the property of enhancing PHA‐induced IL‐2 production by human PBL.

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

摘要:

Summary:The recent availability of recombinant human interleukin‐2 (RIL‐2) has increased interest in the potential clinical use of this lymphokine. We have examined the biologic effects of intermittent bolus and continuous intravenous administration of RIL‐2 in rats. The mean (± SEM) half‐life after an intravenous bolus injection of RIL‐2 was determined to be 2.9 ± 0.5 min (n = 4). The administration of intermittent intravenous bolus injections of RIL‐2 of doses up to 106units/kg every other day for 2 weeks was well tolerated without toxicity as determined by organ histology and serum chemistries. The continuous intravenous infusion of RIL‐2 through an indwelling external jugular vein catheter was tolerated for 2 weeks at doses ≤ 3,000 U/kg/h and was associated with no abnormal serum chemistries or organ pathology. By contrast, animals that received > 10,000 U/kg/h demonstrated RIL‐2 toxicity leading to death of treated rats. Serum chemistries revealed a fourfold increase in serum glutamate oxaloacetic transaminase and serum glutamate pyruvic transaminase. Liver histology revealed hepatocellular necrosis with mononuclear cell infiltration. The thymus was depleted of lymphocytes and lymphoid infiltrates were present in liver, spleen, and lung. This is the first documentation of toxicity secondary to RIL‐2 administration and suggests that hepatopathy may be the dose‐limiting toxicity accompanying the administration of RIL‐2.

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

摘要:

Summary:The antiproliferative effects of six different human interferons were examined on two human cell lines—HM7 (human melanoma cell line) and MDA‐MB‐231 (human breast carcinoma cell line). A dose‐response curve was developed for each interferon in which the maximum dose applied gave at least 30% growth inhibition of control values after 96—128 h of continuous exposure. An amount of &agr;‐difluoromethyl ornithine (DFMO) which caused 25% growth inhibition (0.01 mMfor HM7 and 0.1 mMfor MDA) was added to the cultures with various doses of each interferon. The inhibitory effects of DFMO and each interferon were additive at low concentrations. In no case was a synergistic effect observed. Unlike in the murine B‐16 melanoma model, we could not show a synergistic inhibitory effect between DFMO and any of the six different interferons on two human epithelial tumor cell lines.

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

摘要:

Summary:After mezerein treatment of suspension cultures of the acute monocytic human leukemia cell line THP‐1, cells became adherent to plastic culture surfaces, lost division potential, acquired Fcreceptors, displayed phagocytic activity, and expressed increased nonspecific esterase staining. Serum‐free RPMI 1640 medium conditioned by adherent THP‐1 cells was examined for the presence of biological response modifiers. Preparative isoelectrofocusing of concentrated medium in the presence of various pH gradients of Ampholine ampholytes resulted in the separation of the following activities: fibroblast growth‐stimulating activities in pH ranges of 4.10‐4.55 and 5.30‐5.45; colony‐stimulating factor (CSF) for mouse bone marrow cells at pH 4.10‐4.55; and a malignant cell growth inhibitor comigrating with a lymphocyte‐activating factor (interleukin‐1) at pH 6.70‐6.95. Both CSF and the fibroblast growth stimulator isofocused at pH 4.10‐4.55 coeluted following molecular‐sieve chromatography through P‐100. CSF‐induced colonies were composed of nongranulocytic mononuclear cells. Chromatography through an ACA‐54 column separated interleukin‐1 from most of the growthinhibitory activity.

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

摘要:

Summary:The effect of FK‐565, a novel low molecular weight (MW) acyltripeptide, on tumoricidal properties of murine macrophages is reported here. Peritoneal macrophages (PMs) harvested from C57BL/6 mice and beige mice were rendered cytotoxic to syngeneic B16 melanoma cells following their interaction in vitro with FK‐565. Maximal and reproducible activation of tumoricidal properties in PM were obtained by interaction in vitro with 25 &mgr;g/ml of FK‐565 for a 24 h period, and as little as 0.5 &mgr;g/ml of FK‐565 was sufficient to induce significant cytotoxicity. Murine PMs activated by FK‐565 in vitro were cytotoxic to syngeneic and xenogeneic tumor cells, but did not affect allogeneic nontumor cells. The PMs were also activated to kill B16 melanoma cells by intraperitoneal injections of FK‐565 (10 mg/kg). Multiple injections of FK‐565 into mice also slightly but significantly inhibited lung metastases. These results suggest that FK‐565 has potential as an effective immunopotentiator in immunotherapy.

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID

摘要:

Summary:Three hundred and forty‐five dogs with mammary tumors were initially evaluated for study. One hundred and forty‐four dogs with untreated malignant mammary tumors (136 adenocarcinomas, 5 malignant mixed, 3 sarcomas) were selected for study. All dogs were clinically staged and stratified on the basis of tumor volume and body weight. Dogs were randomized to either a radical mastectomy or simple mastectomy group, and then further randomized to a levamisole or placebo group. There was no significant difference in either survival time or cancer‐free survival time between the surgery groups or between the levamisole and placebo groups. The most significant prognostic factor was tumor volume; dogs with tumors <41 cc had significantly enhanced survival time (p = 0.0007) and cancer‐free survival time (p = 0.0005).

ISSN:0732-6580    

出版商:OVID    

年代:1985

数据来源: OVID