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11. |
ISOTOPE‐LABELLED α‐AMINO‐ISOBUTYRIC ACID AS AN INDICATOR IN CYTOTOXICITY TESTS |
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Acta Pathologica Microbiologica Scandinavica Section C Immunology,
Volume 85C,
Issue 1,
1977,
Page 73-77
Torstein Lyberg,
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摘要:
The effect of rabbit antisera on the uptake and release of α‐amino‐isobutyric acid (AIBA) in LS‐ and P‐388 cells was investigated. In the presence of homologous antiserum and complement, the uptake of AIBA was inhibited. In the absence of complement, no effect of antiserum was seen. Using prelabelled cells, the efflux of AIBA was greatly accelerated in the presence of antiserum and complement. The AIBA‐uptake method was compared with the trypan blue exclusion test and the51Cr release technique. The AIBA‐uptake method was more sensitive in quantitative cytot
ISSN:0304-1328
DOI:10.1111/j.1699-0463.1977.tb03613.x
出版商:Blackwell Publishing Ltd
年代:1977
数据来源: WILEY
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12. |
IN VITRO MODULATION OF HUMAN LEUCOCYTE MIGRATION AND MIGRATION INHIBITORY FACTOR (LIF) ACTIVITY BY CYCLIC 3‘, 5’‐AMP AND CYCLIC 3‘, 5’‐GMP |
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Acta Pathologica Microbiologica Scandinavica Section C Immunology,
Volume 85C,
Issue 1,
1977,
Page 78-80
K. Bendtzen,
J. Palit,
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PDF (174KB)
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摘要:
The effects of cyclic 3′, 5′‐AMP (cAMP) and cyclic 3′, 5′‐GMP (cGMP) on thein vitromigration of human peripheral blood leucocytes under agarose and on the activity of leucocyte migration inhibitory factor (LIF) was studied. Leucocyte migration was not influenced by dibutyryl cAMP, while the dibutyryl derivative of cGMP significantly stimulated cell migration (1 times 10‐5M). LIF‐treated leucocytes partially escaped migration inhibition in the presence of dibutyryl cAMP (1 times 10‐6M), while dibutyryl cGMP was inefficient. If the parent compounds cAMP and cGMP were tested, almost similar results would be obtained, although at higher concentrations of the drugs. These results represent initial experiments with a view to investigating the possible rôle of cyclic nucleotides in the expressi
ISSN:0304-1328
DOI:10.1111/j.1699-0463.1977.tb03614.x
出版商:Blackwell Publishing Ltd
年代:1977
数据来源: WILEY
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