ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1989.tb00509.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryA simple discontinuous Percoll density‐gradient technique was adapted for isolation of granulocytes and mononuclear cells from cats, dogs, horses and cattle. Separation was accomplished at low speeds using a standard tabletop centrifuge. Cell purity was 100% for both granulocytes and mononuclear cells and cell viability exceeded 95%. Percent recovery of leukocytes ranged from 69 to 83

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1989.tb00510.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1989.tb00511.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryDesmopressin acetate (DDAVPR), a synthetic analogue of vasopressin, was slowly administered intravenously to 12 healthy dogs of various breeds and 10 Doberman Pinschers with mild‐to‐moderate type I von Willebrand's disease at a dose of 0.3, 1.0 and 3.0 μg/kg body weight. Plasma von Willebrand factor: antigen was measured by an electroimmunoassay prior to and 30, 60, 90, 120 and 180 minutes after desmopressin infusion. Desmopressin induced only very modest and statistically insignificant increases in von Willebrand factor in both groups. We conclude that the response to desmopressin as measured by circulating von Willebrand factor is much less pronounced in healthy dogs and in Doberman Pinschers with von Willebrand's disease than in hu

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1989.tb00512.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryIdiopathic dyserythropoiesis in a dog was characterized by chronic nonregenerative normocytic normochromic anemia, cellular marrow and abnormal morphology of erythroid precursors. Serum concentrations of Vitamin B12, folate and iron were inconsistent with secondary causes of dyserythropoiesis. The disorder appeared to be distinct from myelodysplastic syndromes described previously.

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1989.tb00513.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SummaryIn 10 newborn Holstein calves, which were initially bottle‐fed on pooled colostrum, there were transient increases in several serum enzymes. Within a few hours, the mean serum γ‐glutamyl transferase (γGT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), acid phosphatase (ACP), and lactate dehydrogenase (LDH) activities increased 62.5, 2.4, 2.0, 1.8 and 1.3 fold, respectively. In two other calves given initial feeds of enzyme‐free pasteurized milk, there were similar increases in serum enzymes, except serum γGT.Correct interpretation of serum enzyme activities in newborn calves must take into account the normal increases which occur after feeding colostrum. Serum γGT was the only enzyme to increase markedly as a result of its absorption from colostrum. The other serum enzymes are presumably released from the tissues of the calf.The increased activities of serum CK observed in some newborn calves probably resulted from trauma during birth or increased muscular activity af

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1989.tb00514.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY