ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1990.tb00539.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryPlatelet function was evaluated in horses with exercise‐induced pulmonary hemorrhage (bleeder) and in control horses (nonbleeder). Platelet aggregation, secretion, and adhesion to rabbit aortic suben‐dothelium were similar for bleeders and nonbleeders. Platelets readily aggregated in response to ADP, thrombin, collagen, and arachidonic acid, but platelet secretion occurred only with high concentrations of thrombin. Platelets readily adhered to rabbit aortic subendothelium and tended to form large thrombi rather than platelet monolayers or aggregates. These data suggest that horses may be predisposed to thrombus formation and subsequent microvascular obstruct

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1990.tb00540.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryType III procollagen peptide (P‐3‐P) is a serum marker for hepatic fibrosis in humans. The utility of a commercially available radioimmunoassay for P‐3‐P was evaluated in the dog. The specificity of the assay was assessed by polyacrylamide gel electrophoresis (PAGE) of canine serum and purified bovine P‐3‐P, followed by Western immunoblotting with rabbit anti‐P‐3‐P serum. The sensitivity was assessed by performing the radioimmunoassay on dilutions of sera from 22 dogs. Polyacrylamide gel electrophoresis of purified bovine P‐3‐P and sera from two dogs suspected of having elevated P‐3‐P concentrations revealed no homologous bands of staining. Western immunoblotting showed marked cross‐reactivity of the high antisera concentrations with several components of the serum proteins, but none corresponding to the purified P‐3‐P.All tested sera from dogs had minimal competitive binding with radiolabeled P‐3‐P in the radioimmunoassay. Dilution curves of dog sera did not parallel either the standard curve or the dilution curve of a known test human serum. There were no statistically different P‐3‐P concentrations in any of the groups of dogs studied.It was concluded that currently available radioimmunoassay kits for the measurement of P‐3‐P in the human are not applicable in the dog. Seemingly, the structure or metabolism of canine P‐3‐P may vary significantly from that of the bovine or human, limiting the sensi

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1990.tb00541.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryBlood from six dogs with in vitro immune‐mediated erythrocyte agglutination resulted in analytical errors in directly measured counting and sizing functions on a multichannel blood analysis system with histogram capability. Errors in the directly measured values, mean cell volume (MCV), and erythrocyte count were attributed to agglutinated erythrocyte particles that persisted during the relatively short reagent contact time of the analysis. Agglutinated particles less than 240 fl were visible on erythrocyte histograms and resulted in a false low erythrocyte count and false high MCV. Agglutinated cell particles greater than 240 fl were not present on the histogram scale. Because these latter particles exceeded the upper threshold, they did not influence determination of MCV, but resulted in a further decrease in the erythrocyte count. As a result, the other dependent erythrocyte indices were in error. These included false low hematocrit and false high mean corpuscular hemoglobin concentration (MCHC), when compared to corrected reference blood values. Similar errors occurred when analyzing blood samples that were agglutinated in vitro by incubating erythrocytes with incompatible plasma. The counting and sizing errors observed with electronic counting techniques were eliminated or greatly reduced by incubating blood in cell counting diluent for 10 minutes followed by analysis on a single channel counter with attached particle size analyzer. Error in erythrocyte measurement on a multichannel system may be anticipated if there is overt erythrocyte agglutination in a blood sample, an abnormally high MCHC is reported by the system, or subpopulations of large volume (agglutinated cells) are observed on a volume distribution histogra

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1990.tb00542.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryCytochemistry was used to differentiate chicken heterophils and eosinophils in blood smears and cytospin preparations of isolated leukocytes. Cytochemical staining also was performed to evaluate any alteration of cell staining characteristics after heterophils had been isolated using discontinuous Ficoll‐Hypaque gradients. Cytochemical staining reactions of heterophils and eosinophils were the same before and after discontinuous gradient cell separation. Characteristic positive reactions did not occur when chicken heterophils were stained with alkaline phosphatase, peroxidase, Sudan black B, acid phosphatase, alpha‐naphthyl acetate esterase, naphthol AS‐D chloroacetate esterase, periodic acid‐Schiff, or Luna's eosinophil granule techniques. Chicken eosinophils stained positively when peroxidase, Sudan black B, acid phosphatase, and Luna methods were performed. In highly cellular cytospin preparations, peroxidase reactions readily distinguished the few contaminating eosinophils (<1%) from the hete

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1990.tb00543.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SummaryA macrocytic hypochromic anemia, with marked reticulocytosis and large numbers of circulating nucleated erythrocytes, was recognized in a 3½‐year‐old male Beagle‐cross dog. A presumptive diagnosis of erythrocyte pyruvate kinase (PK) deficiency was made, even though PK activity from the patient was within the range of activities measured for normal dogs, because the PK activity should have been several times normal in light of the large number of reticulocytes present. The PK activity in a hemolysate from the patient was heat‐labile compared to activities in hemolysates from normal dogs, a finding consistent with results from a previous study of PK‐deficient Basenji dogs. Seven phosphorylated glycolytic intermediates and 2,3‐diphosphoglycerate were measured in protein‐free extracts of erythrocytes from the patient. All were present in concentrations above that present in normal canine erythrocytes, further supporting the diagnosis of

ISSN:0275-6382    

DOI:10.1111/j.1939-165X.1990.tb00544.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY