摘要:

SummaryThe enzymic activity (succinate dehydrogenase, acid and alkaline phosphatase) of alveolar and peritoneal macrophages as well as leucocytic reaction of ground squirrels infected withYersinia pseudotuberculosis(I and III serovar) have been investigated in dynamics from the 1st up to the 30th day. The animals infected with III serovar survived only to the 7th day, while those infected with I serovar survived up to the 30th day after inoculation. A massive influx of leucocytes having peak values (100‐fold increase) on the 3rd day after infection has been found in the peritoneal cavity of the animals infected with I serovar. Moderate leucocytosis in the blood, and insignificant fluctuations in alveolar macrophage number have been established too. An earlier and higher activation of succinate dehydrogenase in alveolar and peritoneal macrophages from animals infected with III serovar in comparison with those infected with I serovar was observed. No differences in alkaline phosphatase activity of alveolar and peritoneal macrophages have been found between the animals infected with I and III serovar. A correlation has been found between the number of leucocytes and changes in the enzymatic activity of the macrophages. A metabolitic transformation was demonstrated typical for different macrophages (peritoneal and alveolar), in the course of this experimental intraperitoneal infection.Obviously, more virulent serovar III ofY. pseudotuberculosisfails to attract leucocytes to the peritoneal cavity sufficiently quickly, so it overcome the local protective mechanisms with consequent systemic cytochemical changes. On the other hand the virulent serovar I attracts leucocytes to the peritoneum and is presumably destroyed by the

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01231.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryProteins secreted byMycobacterium paratuberculosis (M.ptb)during short‐term cultivations were analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and Western(Immuno) blotting. Cultivation in a defined medium containing35S methionine allowed autoradiographic detection of proteins which had been secreted or passively released by actively metabolizingM.ptborganisms. After the first 3 days of cultivation, 4 proteins with molecular weights of approximately 38, 50, 65 and 110 kilodaltons (kd) were detected on SDS gels. Longer incubation up to 12 days resulted in an increased concentration of these proteins as well as in appearance of additional proteins ranging from 14 to over 90 kd. In long‐term (8 – 10 weeks) culture filtrates only two prominent proteins with molecular weights of 30 and 65 kd proteins could be detected. Immunoblot analysis showed that some of the proteins secreted during short‐term cultivations were recognized by sera fromM. ptb‐infected sheep and more significantly by sera from animals which had been immunized with aM.ptblive vaccine strain. The study indicates that during short incubation timesM.ptbmay secrete immunoreactive proteins which are not dominant in long

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01232.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Book reviewed in this article:Loseblatt‐Kommentar der gesamten lebensmittel‐ und weinrechtlichen Vorschriften. Band I. Herausgegeben und erläutert von Prof. Walter Zipfel, Bundesrichter a. D.; Kurt‐Dietrich Rathke, Rechtsanwalt. 69. Ergänzungslieferung.72. Ergänzungslieferung. Stand Juli 1989. C.H. Becksche Verlagsbuchhandlung, München. Rund 780 Seiten kl.73. Ergänzungslieferung. Stand September 1989. C.H. Becksche Verlagsbuchhandlung, München. Rund 610 Seiten kl. 8°.74.Ergänzungslieferung. Stand Dezember 1989. C.H. Becksche Verlagsbuchhandlung, München. Rund 560 Seiten kl.75. Ergänzungslieferung. Stand Mai 1990. C. H. Becksche Verlagsbuchhandlung, Munchen. Rund 660 Seiten kl.76. Ergänzungslieferung. Stand August 1990. C. H. Becksche Verlagsbuchhandlung, München. Rund 544 Seiten kl.77. Ergänzungslieferung. Rund 8430 Seiten. In fünf L

ISSN:0931-1793    

DOI:10.1111/j.1439-0450.1992.tb01233.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY