摘要:

A 25-year-old white woman ingested an unknown quantity of doxylamine succinate and flurazepam. Urine immunoassay screen (EMIT-dau®) was positive for benzodiazopine and negative for phencyclidine. Subsequent gas chromatographic assay of the urine revealed a markedly positive assay for phencyclidine. Doxylamine was ultimately found to be the cause for the false-positive gas chromatographic assay for phencyclidine.

ISSN:0163-4356    

出版商:OVID    

年代:1981

数据来源: OVID

摘要:

We have adapted commercial immunoassay (EMIT®) procedures for dilantin, phenobarbital, theophylline, carbamazepine, tobramycin, disopyramide, quinidine, procainamide,N-acetyl procainamide, and mysoline to two different centrifugal analyzers (Centrifichem and Multistat) using maximum dilutions of reagents to reduce reagent costs to approximately one-tenth of that incurred using the manufacturer's protocol. This savings due to the dilutions of reagents was primarily because of the ability to prolong the reaction time to achieve acceptably large absorbance changes over the absorbance measurement period. Correlation coefficients between values for drugs analyzed by these modified EMIT protocols and values obtained by other methods ranged from 0.927 to 0.994. Within-run precision and recovery values for these drugs were also entirely acceptable. We found that premature mixing of reagents in the rotors of the Multistat system and variable delivery of reagent in the Centrifichem 400 system were both caused by the surfactant in the EMIT buffer. The former problem is being resolved by the manufacturer; the latter problem was resolved by siliconizing the reagent pipet tips.

ISSN:0163-4356    

出版商:OVID    

年代:1981

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1981

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1981

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1981

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1981

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1981

数据来源: OVID