摘要:

Isepamicin, the 1-N-(S-alpha-hydroxy-beta-aminopropionyl) derivative of gentamicin B, is a new aminoglycoside antibiotic, which not only has most of the properties of amikacin but also is effective against several amika-cin-resistant strains of bacteria. The drug was assayed in guinea-pig and human plasma with a high-performance liquid chromatographic procedure using pre-column derivatization with 1-fluoro-2,4-dinitrobenzene and ultraviolet detection. Linearity was established over the range 0.5–40 μg/ml using 50 μl of plasma. Accuracy had a mean relative error of

ISSN:0163-4356    

出版商:OVID    

年代:1991

数据来源: OVID

摘要:

A novel, simple, and very sensitive liquid-chromatographic assay with a coulometric detector has been developed for quantitatingcis-thiothixene (CTX) in human plasma. A reverse phase, 5-μm cyano column (25 x 0.46 cm), a mobile phase of phosphate buffer (pH 2.5) and acetonitrile (40/60 by vol), and a coulometric detector are used for the separation of CTX, and the internal standard, trifluoperazine. CTX and trifluoprazine are extracted from alkalinized plasma inton-pentane-isopropanol (95/5 by vol) and purified by back extraction into perchloric acid. The optimum oxidation potential for the analytes is +0.8 V versus an Ag/AgCl electrode. The detection limit for CTX is 200 pg using 1 mL of plasma and CTX concentrations are linear from 0 to 40 μg/L. The average interassay CV is 9%, and the mean recovery is 99% relative to the internal standard. Possible interferences from various psychiatric and common drugs in the assay have been studied. The assay method was validated by determining the concentration of CTX in the plasma of 100 schizophrenic patients.

ISSN:0163-4356    

出版商:OVID    

年代:1991

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1991

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1991

数据来源: OVID