摘要:

Cyclosporine A (CsA) bioavailability after i.v. and oral administration was studied in six stable liver transplant patients with open and clamped bile drain. CsA levels were determined by the high-performance liquid chromatography method (HPLC). External bile drainage decreased CsA absorption considerably. Clamping the bile drain resulted in higher and earlier peak CsA levels. Clamping the bile drain increased the bioavailability (9.6% vs. 7%,p< 0.05), but even then it was considerably lower than the value of ∼30% generally reported in literature. It is recommended to continue i.v. CsA administration to obtain therapeutic plasma concentrations. Early bile drain clamping or bile refeeding will improve CsA absorption and may also result in continuously therapeutic CsA levels.

ISSN:0163-4356    

出版商:OVID    

年代:1993

数据来源: OVID

摘要:

We report here a reliable high-performance liquid chromatography-ultraviolet assay for routine assay of cyclosporin A (CsA) in whole blood using solid-phase extraction. This assay is linear, between 20 and 2,000 μg/L, with correlation coefficients >0.998 for five consecutive standard curves. All coefficients of variation (CV) were <8% at CsA concentrations of 45, 480, and 1,800 μg/L, with the exception of the between-day CV at 45 μg/L, which was <15%. The relative accuracy of the method is >94% at 45, 480, and 1,800 μg/L. The mean recoveries for CsA and cyclosporin D (internal standard) were 38.2 ± 4.8% (n = 45) and 40.1 ± 6.7% (n = 45), respectively. This method has proven to be reliable and robust in a high-throughput therapeutic drug monitoring laboratory.

ISSN:0163-4356    

出版商:OVID    

年代:1993

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1993

数据来源: OVID