摘要:

Summary: Homogeneous enzyme immunoassay reagents (EMIT) developed for the measurement of amitriptyline and nortriptyline in serum were modified to allow quantitation of clomipramine and desmethylclomipramine. The method was compared to a high-performance liquid chromatographic method. Between-run precision [coefficient of variation (CV)] for clomipramine in the EMIT assay for amitriptyline ranged from 2.6 to 3.2%. For desmethylclomipramine in the nortriptyline assay, the between-run CV ranged from 1.4 to 1.9%. Serum specimens from 43 patients (desmethylclomipramine) and 59 patients (clomipramine) were analyzed by both methods, with good correlation between methods. For clomipramine, recovery ranged from 100 to 102% (0–600 ng/ml range) and was 95–103% for desmethylclomipramine (0–600 ng/ml). The modified EMIT assays offered sufficient reproducibility, accuracy, and correlation with an established method for routine analysis of clomipramine and desmethylclomipramine.

ISSN:0163-4356    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

Summary: A new sensitive analytical method is described for the quantitation of fluphenazine in 1 ml plasma samples after low oral doses of this antipsychotic agent. The drug is isolated by a simple one step extraction technique and analyzed by high-performance liquid chromatography (HPLC) with coulometric detection. The limit of detection for fluphenazine was 10 pg/ml of plasma and standard curves from 25 to 1,000 pg/ml were linear with an overall coefficient of variation (CV) of <5%. The lowest quantifiable concentration of 25 pg/ml could be determined with a CV of 4.7%. The sensitivity of the HPLC assay was such that plasma concentrations of fluphenazine could be followed for 2 days following administration of a single 10 mg oral dose of fluphenazine dihydrochloride to healthy volunteers. Known metabolites of fluphenazine did not interfere in the assay as they eluted with retention times different from that of fluphenazine.

ISSN:0163-4356    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

Summary: A highly selective and specific method for determining caffeine levels in plasma and saliva is described. The method is a microscale procedure that requires only 25 mUl of specimen, although the assay can be performed with as little as 10 mUl of the specimen and is therefore, particularly suitable for monitoring caffeine levels in the neonates. The method is sensitive to 0.5 mUg/ml and is specific for caffeine as none of the other xanthines shows any interference. The method is also fast as treatment of specimen requires only precipitation of proteins and thus is ideal for use in hospital and clinical laboratories.

ISSN:0163-4356    

出版商:OVID    

年代:1989

数据来源: OVID

摘要:

Summary: The predictive performance of a new rapid theophylline assay (Acculevel Theophylline) and its utility as a clinical research tool are described.

ISSN:0163-4356    

出版商:OVID    

年代:1989

数据来源: OVID

ISSN:0163-4356    

出版商:OVID    

年代:1989

数据来源: OVID