摘要:

Abstract.The present work is based on the use of purified blood cells in analysing possible errors in electronic counting. In counting of erythrocytes, no great errors occurred. In counting of platelets a primary diluting fluid prepared from Isopaque® was found useful to obtain constant separation and to avoid correction for hematocrit. In counting of leucocytes the composition of the diluting fluid was essential; saponin caused complete hemolysis and reduced the interference from platelets. A new method was used for calibrating the electronic particle counter, which simplified the technique and reduced the interference from other cells

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07386.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.Erythrocytes in normal autologous plasma formed rouleaux, which increased in speed and degree with the cell concentration, forming a fine‐meshed network of rouleaux at high cell concentrations. In cases of pathologically or artificially induced elevation of the sedimentation rate, a qualitative change of the red cell interaction has been found, characterized by the tendency to “clumping of rouleaux” with a coarse‐meshed network of rouleaux between such

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07387.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.The relation between the erythrocyte sedimentation rate (ESR) and aggregation has been studied by applying different aggregating substances. In normal autologous plasma the ESR increased with the cell concentration due to increased rouleaux formation reaching a maximum size of ESR. At high cell concentrations the ESR was retarded, probably due to formation of a network of rouleaux, which may explain the remarkably small normal ESR. The elevated ESR and the shape of the ESR curves were found to depend on the aggregating capacity present in the fluid, which determines the formation of “clumps of rouleaux” and adhesions between such clu

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07388.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.Arterial blood lactate, pyruvate, plasma β‐hydroxybutyrate and acetone have been followed in 12 patients with diabetic ketoacidosis during the initial 24‐hour treatment period. In all cases the acidosis was fully explained by the accumulation of ketone bodies, and blood lactate and pyruvate never exceeded normal values. A slight rise was, however, observed in blood lactate in some patients during the treatment. Some possible explanations of this phenomenon are discussed. β‐hydroxybutyrate was cleared from the plasma much faster than acetone, and the ratio between the ketone bodies was constantly changing during treatment of the a

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07389.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.A comparative study of the effect of Catapres® (clonidine BAN) and methyldopa has been carried out in 23 patients suffering from severe hypertension (WHO Stage III). Each patient was observed for a period of 5 months, during the course of which the following parameters were investigated: blood pressure in supine and erect position, renal function (51Cr‐EDTA and125Ihippuran clearance), liver function, serum electrolytes, ECG, and ophthalmoscopy. Furthermore the side effects have been recorded. No significant differences in the effect of the two drugs were noted concerning blood pressure, renal function and the other parameters mentioned. Methyldopa presented a higher frequency of severe side effects than did Catapr

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07390.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.Two groups, each of 10 subjects, have been given isocaloric diets for 21 days. The diets were identical with the exception of their fat content. One group was given 40% of the calories as soybean oil, and the other the same amount as MCT oil. Platelet and serum lipids and platelet function tests were evaluated before and after the dietary period. The platelet lipid analysis included estimation of total cholesterol and phospholipids, fractionation of phospholipids by thin layer chromatography and gas liquid chromatographic estimation of the fatty acid and aldehyde composition of the various phospholipids. Platelet function tests included platelet factor‐3 (PF‐3) activity and availability, platelet aggregation induced by collagen, ADP and thrombin and platelet electrophoresis. In subjects given a soybean oil diet a significant reduction in serum total cholesterol and phospholipids occurred. No changes were observed in the platelet cholesterol and phospholipid levels, whereas a significant increase of linoleic acid in choline, ethanolamine and serine phosphoglycerides took place. The relative increase of linoleic acid was most marked in the choline phosphoglycerides. A moderate decrease in palmitic and oleic acids was seen in most phospholipid fractions. PF‐3 activity in platelet‐rich plasma showed a consistent decrease in these subjects, whereas the other platelet function tests showed insignificant changes. In subjects given MCT oil diet no significant changes were observed in serum or platelet lipids, including the phospholipid fatty acids. Increased PF‐3 activity in platelet‐rich plasma exposed to ADP was the only functional disturbance observed in

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07391.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.The case reports of five patients who developed agranulocytosis while taking the antiarrhythmic drug ajmalin are presented. All five responded rapidly to withdrawal of the drug and, in four of the five, antileucocyte ajmalin‐dependent activity was demonstrated in the serum by means of a leucocytotoxicity tes

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07392.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.This case report presents a 27‐year‐old man with paroxysmal tachycardia, which could not be provoked by a submaximal but only by a maximal exercise test. The provoked tachycardia was diagnosed as ventricular on the basis of fusion beats. During a follow‐up period of 5 years the patient has not had any more attacks of dizziness or syncope, and he still is without signs of organic heart di

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07393.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.Three elderly patients admitted to hospital because of suspected malignancy have been diagnosed as disseminated tuberculosis during life. Classical features of disseminated tuberculosis such as miliary mottlings on the chest roentgenogram and choroidal tuberculosis were absent in two of the patients. The difficulties hindering the establishment of the diagnosis of “cryptic” disseminated tuberculosis are emphasized by the patient records. Response to treatment with isoniazide and para‐amino‐salicylic acid is in some cases the only evidence of tuberculosis. Moreover, the early institution of such therapy when other evidence of tuberculosis is lacking may cure the disease before it enters an irreversible state, and is strongly reco

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07394.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Abstract.Plasma triglyceride turnover has been measured in 34 adult healthy human volunteers selected on the basis of normal serum cholesterol and triglyceride concentrations and a normal b.wt. The circulating triglyceride was labelled in vivo with tritiated glycerol and the disappearance of radioactivity was followed up to 24 h. The descending part of the slope was divided into exponential components with the aid of a computer and the one‐exponential resolutions were used for analysis. The enzymatic nature of the triglyceride removal mechanism was confirmed. When it was assumed that all subjects have a common maximal removal velocity (Vmax) level, calculation ofKmvalues indicated that the population under study is definitely composed at least of two and perhaps of three groups. The next analysis was therefore carried out without any presumptions and the conventional enzyme kinetic plots were drawn. These revealed that the two groups differ in regard toVmaxbut not toKm. Two of the subjects did not fit into either of these groups. Group I with a higherVmaxhad a significantly higher turnover rate than group II, which showed a rather uniform distribution of both turnover rate and concentration. The two groups had identical age, sex and b.wt. distribution. There was a definite difference in the triglyceride kinetics between males and females. Both had identical turnover rates, but females showed a lowerKmand, correspondingly, a lower plasma concentration of triglyceride. The well known difference in plasma triglyceride concentration between men and fertile‐aged women is thus accounted for by the more efficient removal system in the latter. It is concluded that the plasma triglyceride concentration in any individual and under any conditions is determined by three factors: (1) the turnover (synthesis) rate, (2) the maximal removal velocity (Vmax), and (3) the Michaelis constant (Km) of the removal enzyme(s). All of these may be influenced by factors like diet, hormones, metabolites and drugs, but obviously the first two are also genetically determined. It is suggested that any classification of hypertriglyceridemias should include an enzyme kinetic analysis, and this will be attempted in the studies in progr

ISSN:0001-6101    

DOI:10.1111/j.0954-6820.1971.tb07395.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY