摘要:

SummaryA group of thirty car painters exposed to vapours and aerosols of paint containing prepolymer and monomer of hexametylene diisocyanate (HDI) was investigated. Specific antibodies against monomer HDI and prepolymerized HDI were analysed with RAST (IgE) and ELISA (IgG) assays after conjugation of the haptens with human serum albumin. There was no significant increase of serum IgG antibodies against HDI monomer, nor of specific IgE antibodies against HDI monomer or prepolymer. Specific IgG antibodies against prepolymerized HDI were significantly increased, as compared with non‐exposed referents (medians 0.11vs0.03 absorbance (A)). Six car painters were found to have specific IgG antibodies of subclass 4 against HDI prepolymer, four also against HDI monomer. This shows an association between exposure and specific IgG antibodies. Thirteen subjects had suffered symptoms of rhinitis and/or conjunctivitis, and ten had symptoms from the bronchi (two asthma). There was no significant association between symptoms and levels of specific antibodies. Most of the symptoms were slight and unspecific, probably due to irritative effects of the exposur

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02847.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummaryThree types of air‐sampling apparatus (the Air‐Sentinel, Burkard Personal Sampler and Casella Personal Sampler) were compared for their use in sampling the air in situations where individuals are exposed to laboratory animals, and a cascade impactor was used to determine the sizes of particles carrying the allergens under investigation. An ELISA, using monospecific antisera raised to selected major allergens of the mouse, rat and rabbit, i.e. mouse Ag 1 (mouse urinary prealbumin), rat Ag 4 (rat urinary prealbumin) and rabbit Ag R1, was developed to assay the extracts obtained from the samplers. This ELISA system was able to detect<5 ng/m3of allergen in sampled air. The rat and mouse allergens were shown to be carried mainly on particles of 6–18 μm, whereas the rabbit allergen was also found on particles of 2 μm and smaller. Allergen levels correlated well with the number of animals present in the room and the degree of activity during sampling. A protective filtered‐air hood, when worn during surgical operation procedures, was shown to reduce effectively the level of rat allergen breathed by an i

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02848.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02849.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY