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11. |
Absence of IgG antibodies to TDI‐HSA in a radioimmunological study |
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Clinical&Experimental Allergy,
Volume 13,
Issue 1,
1983,
Page 75-79
P. L. PAGGIARO,
M. FILIERI,
A. M. LOI,
M. G. ROSELLI,
R. CANTALUPI,
A. PARLANTI,
G. TOMA,
L. BASCHIERI,
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摘要:
SummarySera of ninety‐one workers with respiratory symptoms related to occupational exposure to toluene diisocyanate (TDI) were tested by a solid phase radioimmunoassay using polyvinyl plates, in an attempt to demonstrate specific IgG antibodies to a conjugate of TDI with human serum albumin (TDI‐HSA). Different conjugates were prepared. In our radioimmunoassay a TDI‐HSA 1600 (1600 μg TDI/ mg HSA), obtained at alkaline pH with borate buffer, was used; this conjugate was immunologically reactive and did not contain protein macro‐aggregates.No difference in percent of binding of radiolabelled anti‐human IgG to TDI‐HSA coated on plastic plates was found between exposed workers (asthmatic and non‐asthmatic) and fifteen normal subjects. This may suggest that either TDI‐induced bronchial asthma is not mediated by IgG antibodies, or TDI‐HSA conjugate is unsuitable to be detected by a
ISSN:0954-7894
DOI:10.1111/j.1365-2222.1983.tb02570.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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12. |
An in vitro study of lymphocytes in patients with atopic dermatitis |
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Clinical&Experimental Allergy,
Volume 13,
Issue 1,
1983,
Page 81-88
E. VALVERDE,
J. M. VICH,
J. HUGUET,
J. V. GARCÍA‐CALDERÓN,
P. A. GARCÍA‐CALDERÓN,
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摘要:
SummaryThe authors studied the T‐ and B‐lymphocyte sub‐populations, the stimulation of the lymphocytes with phytohaemagglutinin and pokeweed mitogen, the non‐specific T suppressor activity and the amounts of serum IgE in a group of thirty‐four patients with atopic dermatitis. Comparison with a group of 100 healthy controls revealed a diminution in the T sub‐population, an increase in the B sub‐population, no change in the response index to stimulation with phytohaemagglutinin or pokeweed mitogen, a diminished T‐suppressor activity and an increase in the lev
ISSN:0954-7894
DOI:10.1111/j.1365-2222.1983.tb02571.x
出版商:Blackwell Publishing Ltd
年代:1983
数据来源: WILEY
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