ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00141.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00142.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00143.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00144.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryA double‐blind controlled trial of hyposensitization with tyrosine‐adsorbedDermatophagoides pteronyssinusvaccine in 24 children with atopic eczema and immediate hypersensitivity toD. pteronyssinusfailed to demonstrate superiority over placebo after a standard 8 month course of treatment.In a second phase, children initially administered active treatment were randomly allocated to continue with active treatment or switched to placebo for a further 6 months. The clinical scores suggest that prolonged hyposensitization may be more effective than placebo but the numbers were too small to permit confident conclusions. A dramatic placebo effect may have served to conceal any additional therapeutic effect from active treatm

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00145.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryExperimental studies have shown thatN(3′, 4′‐dimethoxycinnamoyl) anthranilic acid (Tranilast) inhibits reaginic antibody‐mediated hypersensitivity reactions, and it has been demonstrated to be an effective drug for patients with bronchial asthma. On the other hand, from the nature of the cellular infiltrate seen in eczematous lesions, it appears that some form of cell‐mediated immunity may be involved in addition to IgE‐mediated immunity in the pathogenesis of atopic dermatitis (AD). Moreover, we have previously reported that the proliferative responses of peripheral blood mononuclear cells (PBMCs) to ovalbumin (OA) or bovine serum albumin (BSA) in children with AD who are sensitive to hen's egg or cow's milk were significantly higher than those of healthy children and hen's egg or cow's milk sensitive children with immediate symptoms.In this study, we have showed that the proliferative responses of PBMCs to OA were dose‐dependently inhibited by Tranilast on patients with AD. The responding cells to OA were shown, through separation experiments, to be T cells, and the proliferative responses of T cells to OA were also dose‐dependently inhibited by Tranilast. Moreover, the inhibition was thought to occur at the initial stage of the proliferative reactions. These results suggest that Tranilast can be clinically applied to p

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00146.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryA major allergen of the storage miteLepidoglyphus destructor (Lep dI) has been purified by affinity chromatography using an anti‐Lep dI monoclonal antibody. The purity of the protein obtained by this procedure was assessed by reverse‐phase HPLC.Lep dI displayed a molecular weight of 14 kD on SDS‐PAGE under non‐reducing conditions, and 16 kD in the presence of a reducing agent. Analytical IEF revealed a little charge microheterogeneity, showing three bands with pIs 7.6–7.8. PurifiedLep dI retained IgE‐binding ability, as proved by immunoblotting experiments after SDS‐PAGE and RAST with individual sera fromL. destructor‐sensitive patients. Results from the latter technique demonstrated that 87% ofL. destructor‐allergic patients had specific IgE toLep dI, and a good correlation between IgE reactivity withL. destructorextract andLep dI was found. In addition, RAST inhibition experiments showed that IgE‐binding sites onLep dI are majorL. destructor‐allergenic determinants, sinceLep dI could inhibit up to 75% the binding of specific IgE toL. destructorextract; on the other hand.Lep dI did not cross‐react withD.

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00147.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe protein and allergen contents of four commercial soybean skin test extracts were tested by SDS‐PAGE and immunoblotting using sera from soy‐allergic adults. Polyacrylamide gels stained with Coomassie Blue showed an absence of several major soybean proteins, particularly those at higher molecular weights. The acidic subunits of glycinin and beta‐conglycinin, major soybean storage proteins, appear to be absent or present in much reduced amounts. Immunoblots with soy‐allergic sera indicate alteration, reduction, or loss of IgE‐binding in the commercial extracts as compared to extracts of soy flour. In one soy‐allergic patient, skin tests revealed a negative response to three of the commercial soybean extracts and a mild response to one extract. Defatted soy flour obtained from two of the four extract manufacturers was extracted in the laboratory using a standard procedure for the isolation of soybean proteins. In one case, the extract still had an abnormal protein profile on gel electrophoresis while in the other case, the new extraction procedure gave significantly improved extraction of soy protein. Preparation methods appear to be partially responsible for the variable allergen content in commercial soybean skin te

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00148.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummarySera from 13 patients with positivePityrosporum ovaleskin prick test were analysed with IgE immunoblotting. BothP. ovaleandCandida albicansantigens were used to reveal possible crossreactivity of these yeasts. Of the 13 sera, eight sera showed IgE binding toP. ovaleand six sera toC. albicans.Simultaneous IgE‐binding to bothC. albicansandP. Ovalewas observed in five of these sera. The most common IgE‐binding band pairs were, a 23 kD band ofP. ovaleand a 27 kD band ofC. albicans, as one pair, and a 26 kD band and a 13 kD band, respectively, as another pair. In addition to these protein bands, simultaneous reactivity was observed toC. albicansmannan, a polysaccharide, and to a diffuse high molecular weight component ofP. ovale, possibly also a polysaccharide. The five sera with simultaneous IgE‐binding were analysed with RAST‐inhibition, which revealed presence of crossreacting IgE‐antibodies toP. ovaleandC. albicansin two of these sera. Crossreacting epitopes were suggested to be located in the mannan polysaccharide ofC. albicansand high molecular weight diffuse stain ofP. ovale, based on the immunoblotting and RAST‐inhibition patterns of the studied sera. Crossreacting sera were pooled and used as an IgE probe in CRIE and Tandem‐CRIE. These experiments revealed a fused precipitin line indicating presence of a common structure ofP. ovaleandC. albicans.This suggested crossreactivity may imply an interesting phenomenon in atopics who are sensitized byC. albicansgrowth in the gastrointestinal tract and get exposed byP. ovalegrowing on the skin. Further studies are thus needed to clarify the clinical significance of these prelimi

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00149.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe MAGIC LITE system, a newly developed immunochemiluminometric assay for specific and total IgE antibody using paramagnetic particles coupled with standardized allergens as solid phase, was compared to the CAP system, a recently introduced immunoassay based on a cellulose polymer encased in a capsule. A total of 357 serum samples of patients with suspected inhalant allergies and a positive skin prick test (SPT) to common allergens (birch, timothy‐grass, mugwort, cat dander,Dermatophagoides pteronyssinus, Alternaria) were investigated. Fifty SPT negative subjects served as controls (total number of tests in each assays = 1600). Both assays were highly precise (overall intra‐assay and inter‐assay coefficients of variation were 2.9% and 4.5% in MAGIC LITE, 4.7% and 5.5% in CAP) and showed excellent linearity (meanr2of eightfold log2serum dilutions were 99.7% and 99.3% in MAGIC LITE and CAP). Good correlations were found between the absolute specific IgE antibody values detected by both methods (correlation coefficientr: birth 0.86, grass 0.93, mugwort 0.96, cat 0.91,D. pteronyssinus 0.73, Alternaria 0.90). Excellent specificity (.98%) occurred in both assays and with all allergens, and sensitivity was related to the allergen (MAGIC LITE/CAP): birch 91%/89%, grass 83%/90%, mugwort 50%/69%, cat 83%/83%,D. pteronyssinus72%/78%, Alternaria 75%/81 %. Our results indicate that bothin vitrotests are useful tools for the detection of specific IgE ant

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1992.tb00150.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY