ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02892.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummaryNasal provocation with ragweed pollen extract was performed on ragweed‐sensitive and non‐atopic subjects. Nasal lavage fluids were collected 15 min after saline and allergen challenges, and assayed for total protein, albumin, potassium, lysozyme activity and peroxidase activity. There was no statistically significant increase in any of these lavage fluid constituents in non‐atopic subjects after allergen provocation, compared with after saline provocation. The lavage fluids of ragweed‐sensitive subjects had significant rises in each of the constituents following allergen provocation. This method provides a simple mechanism for quantitating the nasal secretory response to allergen prov

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02893.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummaryBromelains consist of a group of proteolytic enzymes of Bromeliaceae. They are commonly used in pharmaceutical industries, food production and in diagnostic laboratories. Bromelains are known to cause IgE‐mediated reactions of both the immediate type and the‘late phase reaction of immediate type reaction’ with predominantly respiratory symptoms. We report four cases of occupational allergy to bromelain in workers of a blood grouping laboratory. These observations prompted us to investigate the sensitization rate to bromelain in all workers of the particular diagnostic laboratory who had contact with bromelain. These results were compared with those obtained from healthy, randomly selected individuals without evident bromelain exposure. Our findings indicate that (i) bromelain is a strong sensitizer, (ii) sensitization usually occurs due to inhalation and not to ingestion, (iii) bromelain allergy is occupationally acquired, and adequate precautions are necessary. We can further state that (iv) skin testing with relatively pure allergens such as isolated proteases like bromelain may induce systemic reactions, even at very high dilu

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02894.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummaryTwelve workers with hard metal asthma diagnosed on the basis of peak flow diaries and positive bronchial reactions to cobalt chloride (CoCl2) were studied for sensitization by detection of specific antibodies to radioactive cobalt (57Co), cobalt‐conjugated human serum albumin (Co‐HSA) and cobalt‐conjugated exchange resin (Co‐resin). Their IgE titres ranged from 73 to 1500 IU/ml and eight were atopic individuals. Sixty serum samples from asthmatic patients with IgE titres of 14–4300 IU/ml were studied as controls in all tests. Eleven of twelve subject sera that selectively bound to57Co after incubation with saturated ammonium sulphate (>232 c.p.m.,P673 c.p.m.,P417 c.p.m.,P<0.01), and (3) four sera that were negative for two antigenic agents (Co‐HSA, Co‐resin). These results suggest that the subjects had occupational asthma due to hard metal exposure from cobalt sensitivity. An immuno‐allergic mechanism mediated by specific IgE antibodies to cobalt was confirmed to be responsible for the development of hard metal asthma, with the possibility of some role of the r

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02895.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummaryThere have been reports of elevated venom‐specific IgE levelsin post‐mortemsera frompatients experiencing fatal Hymenoptera stings so wehypothesized that other cases of sudden death may be due to unrecognized sting anaphyiaxis. Of 94 sera obtainedpost mortemfrom subjects who died unexpectedly during the summer months, 22 (23%) contained elevated levels of IgE antibody to at least one insect venom. The causes of death as determined byautopsy did not differ significantly among patients with orwithout elevated IgE antibody levels. An identical percentage of seropositivity was noted in 48post‐mortemsera from victims of sudden, unexpected death in the winter months. Conversely, only 6% of sera from 92 living blood donors contained elevated venom‐specific IgE antibodies. The appreciable incidence of elevated IgE antibodies in thepost‐mortemgroups suggests that sting anaphylaxis should be more highly considered as a possible cause of unexpec

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02896.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummaryIn this study 51 children who presented with symptoms of cows’ milk allergy (CMA) were categorized clinically by their response to cows’ milk challenge. Forty‐two patients had unequivocal evidence of CMA and nine were milk tolerant. Of the patients with CMA two groups were identified. The first, made up of 32 patients, had immediate‐type hypersensitivity reactions to milk associated with both positive skin‐prick test (SPT) and RAST. The second group of 10 late reacting patients developed symptoms of CMA over several hours or days; significant increases in irritability, frequency of bowel actions, and rhinitis following milk ingestion were noted in this group. Leucocyte inhibition factor (LIF) produced in response to α‐lactalbumin, β‐lactoglobulin and α‐casein was assessed in the immediate and late reacting CMA patients as well as in the milk‐tolerant group. There was no difference in LIF production between the milk‐tolerant group and those with immediate reactions. However, these two groups produced less LIF than the late reacting patients for α‐lactalbumin (P= 002), α‐casein (P= 0.03) and β‐lactoglobulin (P= 0.05). A clinical diary score card was found to be a useful instrument to assess the response of non‐imme

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02897.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummaryIn a study of cows’ milk allergy (CMA) in infancy, 135 consecutive challenges were performed on children with a good clinical history of the disorder. Of these, only half of the patients were shown to have the disease. Highly atopic patients responded rapidly to small volumes of milk with acute urticaria, wheezing, stridor and eczema, whereas patients who were relatively non‐atopic developed symptoms of eczema, bronchitis and wheezing over several hours or days. In a statistical evaluation of the diagnostic value of skin tests and RAST it was shown for the extracts used in this investigation, and for the population studied, all patients with SPT 4 had CMA. The results highlight the potential diagnostic value of SPT in the identification of children with some forms of CMA if standardized cows’ milk allergen extracts can be pre

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02898.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummarySixty‐one sera with positive RAST to mugwort pollen (Artemisiae vulgaris) were submitted to RASTs for birch pollen (Betula verrucosa) andcelery (Apium graveolens). In 36 cases RAST results were positive for celery. In addition, 23 sera presented specific IgE to birch pollen. The binding of specific IgE to individual allergens in celery, mugwort pollen and birch pollen was studied by the immunoblotting technique. This involved electrophoretic separation of allergenic extracts, electrotransfer of proteins onto nitrocellulose sheets and sensitive immunoenzymatic detection. Eighteen sera had specific IgE binding to two celery components of molecular weight around 15 kD. All these sera also detected a 15 kD allergen in mugwort and two allergens in birch of 14 kD and 16 kD molecular weight. The sera that did not detect the 15 kD bands in celery failed to react with both the 15 kD mugwort component and the 14 and 16 kD birch components. Specific cross‐inhibitions of the detection of these allergens on immunoblots were obtained by pre‐incubation of the sera with crude extract of the three species. These results strongly suggest that such allergens display some structural identity and that they could be at the origin of some cases of crossed hypersensitivity to celery, mugwort pollen and birch p

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02899.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

SummaryThirty‐eight adults with allergic rhinitis have been treated with a new partially purified extract of house dust mite (Dermatophagoides pteronyssinus) in a double‐blind placebo‐controlled trial. Patients were randomized to active (Pharmalgen®,D. pteronyssinus) and placebo (histamine) treatment by sensitivity toD. pteronyssinuson nasal challenge. In the actively treated group nasal symptoms, assessed by visual analogue score, improved (P<0.01), sensitivity on nasal challenge with allergen was reduced (P<0.05) and weal size on skin‐prick test with allergen was reduced (P<0.01), compared with the placebo group. These results occurred after 3 months of treatment. Reduction in target organ sensitivity occurred, while the serum level ofD. pteronyssinusIgE rose in the active group from 14.2 to 22.5 PRU/ml (geometric mean) but did not change significantly in the placebo group. As anticipated, because of the treatment schedule used, a number of generalized allergic reactions were induced by injections, but all responded promptly and easily to treatment. These results suggest this is an effective form of therapy, which now offers us the opportunity to study the immunological mechanisms of hyposensitization and to devise a modified schedule causing fewer r

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02900.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1988.tb02901.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY