ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00357.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00358.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00359.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryTo further characterize airborne cat allergen and a newly established cat challenge facility, airborneFel dI levels and particle size distributions were studied in both the cat challenge room and home environments under different conditions of ventilation and physical activity. In the cat room, there has been a dramatic and continued rise in the concentration of airborneFel dI since the room was established. No differences in total airborneFel dI levels or particle size distribution were detected under widely differing rates of ventilation (40 air changes per hour vs 8 ac/hr vs17 μm. Although very little allergen f<15%) was detected on particles<4 μm, this important fraction was present under all conditions. We conclude that airborne cat allergen resides primarily on relatively large particles, that a small but consistent fraction is found on very small particles, and that neither allergen levels or particles size distribution are significantly influenced by ventilat

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00360.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryEighty‐two children admitted to hospital with exacerbations of asthma were studied to determine how many were exposed to house dust mites at the time of admission and displayed immediate hypersensitivity to house dust mites. The concentration of house dust mite allergen (Der pI) was measured in dust obtained from the child's mattress, bedroom floor and living room floor. Sixty‐two (75%) children admitted had been exposed to>10 μgDer pI/g. Sixty‐seven (82%) children were sensitive to house dust mite(RAST 1 +, or weal 3 mm): 49 (60%) children were both exposed and sensitive. In contrast in a control group of 44 children, 31 (70%)(n.s.) were exposed to>10μgDer pI/g, 10 (23%) (P<0.001) were sensitive to house dust mite, and 7 (16%) (P10μgDer pI/g (P<0.001). In conclusion, the majority of children admitted to hospital with exacerbations of asthma were exposed to house dust mite allergen and were house dust mite sensitive. Further the results suggest that continued exposure to higher concentrations of mite allergen may be associated with the risk of

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00361.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryA two‐site monoclonal antibody (MoAb) ELISA has been developed for the quantification of thePhleun pratensemajor allergen,Phl pV. The assay is based on two MoAbs which recognize different non‐overlapping epitopes on thePhi pV molecule; one antibody (1D11) was immobilized on the solid phase and the other (3B2) was biotinylated. An affinity‐purifiedPhi pV preparation (purity of 95%) was used as standard. The assay has a sensitivity of 10 ng/ml of allergen and is suitable for the detection of group V allergen in aqueous grass extracts. The specificity of the assay was investigated with 14 grass pollen and five non‐grass pollen extracts. Different levels of group V allergen were delected in extracts of grasses, but not in non‐grasses. The assay gives a good correspondence with allergenic activity of extracts as determined by ELISA inhibition using serum pool of allergic patients. The results indicate that the two‐site MoAb ELISA could be very useful in the standardization of allergenic extracts from g

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00362.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryType I allergy to pollen of the European chestnut (Castanea sativa) represents a major cause of pollinosis in (sub) Mediterranean areas. Using sera from 14 patients with established allergy to pollen of the European chestnut, 13/14 sera (92%) showed IgE‐binding to a 22 kD protein, 2/14(14%) displayed additional binding to a 14 kD protein and 1/14 (7%) bound only to the 14 kD protein of European chestnut pollen extract. Two monoclonal mouse antibodies, BIP 1 and BIP 4, directed against different epitopes ofBet vI (the major birch pollen allergen), and a rabbit antibody to recombinant birch profilin (rBet vII) were used to characterize the proteins of the European chestnut pollen. The recombinant birch pollen allergens, rBet vI and rBet vII (profilin) were employed to show common allergenic structures on proteins from both birch and European chestnut pollen by IgE‐inhibition experiments. Despite the fact that the 22 kD protein displayed a higher molecular weight in comparison to the 17 kD major birch pollen allergen,Bet vI, we could demonstrate reactivity of both monoclonal antibodies, BIP 1 and BIP 4, with this protein. A complete inhibiton of IgE‐binding to this 22 kD protein was shown by pre‐incubating sera with purified recombinantSetr I. In addition, the 14kD protein could be identified by IgE‐inhibition studies with recombinantBet vII and by using a rabbit anti‐profilin antibody as the profilin from pollen of the Europe

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00363.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryComponents of the renin angiotensin system, namely renin, angiotensinogen, angiotensin I and II and aldosterone were measured in plasma of patients with hymenoptera venom anaphylaxis (n= 50) and healthy non‐allergic controls (n= 25). Patients with a history of anaphylactic reactions to hymenoptera venom who did not undergo immunotherapy showed significantly reduced renin, angiotensinogen, angiotensin I and angiotensin II in plasma as compared with controls (P<0.05). There was no difference in the aldosterone concentration between patients and controls. Angiotensin I, angiotensin II, renin and angiotensinogen levels were the same in male and female patients. There was also no difference in the angiotensin I, II, renin or angiotensinogen levels between young and older patients. A significant inverse correlation between the severity of clinical symptoms and the plasma levels of renin (r=‐0.382,P<0.001), angiotensinogen (r=‐0.567,P<0.0001), angiotensin I (r= ‐0.656,P<0.0001) and angiotensin II (r = 0.0762,P<0.0001) was found: the lower the levels the more severe the clinical symptoms. No correlation was found for aldosterone. Hymenoptera venom allergic patients with repeated anaphylactic reactions during hyposensitization did not tolerate the sting of a living insect (n= 6). In these patients, renin, angiotensinogen, angiotensin I and II remained significantly lower than in healthy non‐allergic controls. Patients with successful immunotherapy (n= 27) who tolerated the sting of a living insect had renin, angiotensin I and II significantly higher than patients without immunotherapy. These findings suggest a possible role of the renin angiotensin system in hymenoptera venom an

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00364.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryTo evaluate the presence of phosphatidylinositol‐4‐phosphate in peripheral blood eosinophils, venous blood was drawn from normal healthy volunteers. The eosinophils were isolated on a Percoll gradient and were incubated with [γ3P] ATP in the presence of Mg2+. After stopping the reaction, lipid extraction was performed with acidic medium and phospholipids were separated by thin‐layer chromatography on 1% (w/v) oxalic acid‐ and potassium oxalate‐impregnated silica gel plates. Considerable amounts of radioactivity were found to be incorporated into phosphatidylinositol‐4‐phosprmte on both plates. This reaction requires ATP and Mg2+, and maximal response is seen at 10 μmATP and 20 mm Mg2+. The reaction is dependent upon the time and temperature of the assay system. No significant superoxide anion generation from the eosinophils incubated with ATP at the concentrations used in the study was observed. These results suggest the possible presence of phosphatidylinositol kinase which catalyses the formation of phosphatidylinositol‐4‐phosphate from endogenous phosphatidylinositol in human periphera

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00365.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryA serine protease from mite faecal extract,Dermatophagoides farinae, was purified using DEAE‐Sephacel anion exchange chromatography and Supcrdex 75 pg gel chromato‐graphy. The molecular weight of this protease was 34 kD on SDS‐PAGE under reducing conditions. The optimal pH and temperature of the protease were 8‐0 and 47 C, respectively. In addition, this protease cleaved arginyl or lysyl residue containing substrates selectively and was only inhibited by aprotinin, PUT‐175, tind soy bean trypsin inhibitor and not by chymostatin, E‐64 and iodoacetic acid. These results show that our purified serine protease belongs to the trypsin‐type. Purified trypsin‐like protease was shown to be allergenic by enzyme‐linked immunosorbent assay. Antigeni‐city of trypsin‐like protease was completely different from thoseof Der fI andDer fII. Both, 20N‐terminal amino acid sequence and amino acid compositions of the purified protease were very similar to those ofDer fIII. Good similarities were found between trypsin‐like protease andDer fIII concerning physicochemical properties such as molecular weight on SDS‐PAGE and ammonium sulphate solubility. Summarizing the above data, it can be concluded that a trypsin‐like protease from mite faecal extract is actually theDer fIII allergen and that it may be involved in the digestive process of the mite as it was found not in

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb00366.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY