ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03230.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03231.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03232.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03233.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03234.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03235.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryTotal RNA was extracted from the whole body of American cockroach Periplaneta americana) using chaotropic salt guanidine isothiocyanate in the presence of 2‐mercaptoethanol (2‐ME). Polyadenylated mRNA was isolated by oligothymidylic acidcellulose chromatography and mRNA was translated using a rabbit reticulocyte lysate system. The translation, as judged by the incorporation of‐S‐tnethionine, was obtained with poly(A)‘*’RNA, where an approximately 9‐5‐fold increase in label incorporation over control was achieved. Analysis of translation products by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) in combination with autoradiography showed that many proteins with apparent molecular weights ranging from 12 to 200 kD were synthesized, and no labelled proteins were found with negative RNA control and poly(A) RNA. Immunoprecipitation studies performed using polyclonal and monoclonal antibodies revealed that synthesized proteins of MW 90, 78, 72, 49, 45, and 26 kD corresponded with previously identified principal and major allergens of American cockroach from our laboratory. In addition, the allergenicity of the translation mixtures was also confirmed by fluoroallergosorbent test (FAST) inhibition studies with IgE antibodies of huma

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03236.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryThe role of IL‐4 on IgE synthesis has been well established. IL‐4 has been shown to promote IgE production by B cells from atopic and non‐atopic donors. In this study, the effects of natural exposure to pollens on IL‐4‐indueed IgE synthesis by peripheral blood mononuclear cells of atopic and non‐atopic donors were examined. The results confirm production of IgE in an IL‐4 dose‐dependent manner by PBMC cultures of these two groups. When cultures were performed out of the pollen season, following stimulation by IL‐4, no significant difference was observed between the levels of IgE produced by PBMC of atopic and non‐atopic donors. In contrast, upon natural exposure to pollens, significant higher levels of IgE were measured in the atopic group than in the non‐atopic one. These results show that the policn season infiuences the IL‐4‐induced IgE synthesis by PBMC of allergic patients and are in keeping with seasonal rise o

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03237.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryThe effect of genetic and environmental factors on the prevalence of allergic disorders in early childhood was determined in a prospective follow‐up study. Information was available on 1174 children at the age of 2 years. Two‐hundred and seventy‐five were considered to have an allergic disorder. The prevalence varied from 3.2% for rhinitis to 10.9%, for asthma. At 2 years 60 children reacted positively on skin‐prick test (SPT). Multivariate logistic regression analysis was used to obtain adjusted odds ratios (95% confidence interval) for each factor. For asthma, positive family history, male sex, low birth‐weight, maternal smoking and season of birth were significant risk factors. For eczema, positive family history was the only significant risk factor. For rhinitis, lower socio‐economic group and autumn birth were significant. Maie sex and low birth‐weight were significant for skin test positivity. Positive family history and low birth‐weight were significant risk factors for any allergy. Low birth‐weight was also a significant risk for skin test reactivity to house dust mite. Genetic and environmental factors have a profound effect on the development of allergic disorders in the first

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03238.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

SummaryWe have reported the presence of a histamine synthesizing enzyme, histidine decarboxylase (HDC), and histamine degrading enzymes, histamineN‐methyltransferase (HMT) and histaminase (diamine oxidase, DAO) in human nasal mucosa and the histamine content of the mucosa. In this study, we demonstrate the influences of the toluene diisocyanate (TDI) treatment on the histamine content and these enzyme activities in guinea‐pigs as an animal model of respiratory hypersensitivity. Application of TDI to the nasal vestibuli induced intense nasal allergy‐like and mild asthma‐like responses in TDI‐sensitized guinea pigs. Increases in the histamine content and HDC and HMT activities were observed in the nasal mueosa and lung of TDI‐sensitized guinea pigs. No apparent changes in the histaminase activities were observed in either the nasal mucosa or the lung. These data suggest that the turnover rate of histamine is increased in the nasal mucosa and the lung of guinea pigs with respiratory hyper

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1993.tb03239.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY