ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01973.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01974.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryAsthmatic patients have a deficiency of concanavalin A‐(Con A) induced suppressor cell function. We tested whether oral colchicine 0·5 mg twice daily for 7 days could correct this immunoregulatory abnormality. Peripheral blood mononuclear cells were incubated with Con A and then suppression of proliferation was measured by co‐culture of these cells with healthy volunteers’mononuclear cells and phytohaemagglutinin. Sixteen asthmatic patients had significantly (P<0·002) decreased Con A‐induced suppressor cell function (17·0±17·2%, mean ± s.d.) as compared to 13 healthy volunteers (37·9±14·9%). Oral colchicine significantly (P<0·05) increased, though only partially corrected, these 16 asthmatic patients’Con A‐induced suppressor cell function (28·1±14·3%). Asthmatic patients had an increased number of monocytes (691±289 vs 388±271/mm3for normals,P<0·01) and a normal number of lymphocytes, Leu 4+ total T cells, Leu 3+ helper/inducer T cells, and Leu 2+ suppressor/cytotoxic T cells as well as a normal Leu 3/Leu 2 ratio. Oral colchicine significantly (P<0·005) decreased the number of monocytes (451±255/mm3) without significantly affecting the number of lymphocytes, Leu 4+, Leu 3+, or Leu 2+ T cells, or the Leu 3/Leu 2 ratio. These results are consistent with the hypothesis that the deficiency of Con A‐induced suppressor cell function in asthmatic patients may be due, in part, to an increased number and/or abnormal activity of monocytes. If so, then oral colchicine may have partially corrected the deficiency of Con A‐induced suppressor cell function by decreasing the number and/or mod

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01975.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryChildren with or without cow's milk protein intolerance were investigated for serum antibodies to native cow's milk proteins, processed cow's milk proteins and native‐ and pepsin‐digested β‐lactoglobulin. Antibodies of IgG and IgA isotypes were determined with ELISA and those of IgE isotype with RAST.Children who exhibited slowly appearing untoward reactions to cow's milk feeding had significantly higher titres of IgG antibodies against both native and digested β‐lactoglobulin than children with an immediate type of reaction or no intolerance. The IgG antibodies to pepsin‐digested β‐lactoglobulin were efficiently inhibited by native β‐lactoglobulin even at low concentrations, which suggests that there were no antibodies specific for the degraded proteins. The children with slow reactions to cow's milk also tended to have higher antibody levels of the IgG and IgA isotypes to both native and processed cow's milk. Antibodies to this mix of antigens discriminated less well, however, than the antibodies to isolated β‐lactoglobulin between the groups of children with slowly appearing reactions, with immediate reactions and the controls. Seven out of nine children with an immediate type of reaction to cow's milk protein had IgE antibodies against cow's milk protein determined with the RAST, while this type of antibodies could not be demonstrated in any child in the two other groups. Using processed or digested protein as antigen did not increase the sensitivity of the ant

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01976.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryIn 105 subjects taken from a student population and aged between 15 and 30 there was a strong positive association between the presence of the atopic state, defined by skin tests, and a high level of non‐specific bronchial responsiveness to methacholine (χ2= 10·5, d.f. = 2,P= 0·01). Regression analysis showed a history of asthma, and the symptom of wheeze, to be predominantly predicted by the degree of bronchial responsiveness (R2= 31%), with only a minor independent contribution from the degree of atopy (R2a furthur 5%). The genetic or other reasons for the association between bronchial responsiveness and atopy may have importance in understanding the aetiology of allergic as

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01977.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryOne hundred and six adults with various reactions to yellow jacket (YJ), honey bee (HB) or unidentified insects (UI) were tested for allergy to insect venoms. For various reasons none received immunotherapy. Individuals completed questionnaires annually for three consecutive years and described sting reactions within the previous season. Ninety subjects completed all the questionnaires and 77 of these were re‐tested at the end of the period. Nine out of 25 patients reacted with a systemic reaction when re‐stung. High IgE and low IgG venom‐specific antibody levels indicated an unfavourable prognosis, since eight of 11 individuals who initially presented venom‐specific IgE>RAST class 2 and venom‐specific IgG below detection limit had systemic reactions at re‐sting. No such reactions occurred in subjects with no specific IgE and only one out of six with specific IgE as well as IgG reacted systematically. Skin prick tests (SPT) of<3mm with YJ venom 1,000 μg/ml excluded later systemic symptoms to stings, whereas larger skin reactions gave an equal chance of systemic or local reactions at re‐sting. In individuals not stung by UI, YJ and/or HB the decline in venom‐specific IgG and IgE was significant, median values ranging from 41% to 75% over the 3‐year period. The decline was unaffected by the type of sting reaction prior to the initial test. SPT results did not change significantly. The findings are relevant when testing patients several years after their last insect sting and the results might indicate that the antibody decline is accompanied by a decrease in cl

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01978.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryTerfenadine suspension, 30 mg b.i.d., was compared with placebo in a randomized, double‐blind cross‐over study in twenty‐five children, 6–12 years of age, with grass pollen induced allergic rhinoconjunctivitis. The patients were treated during two 7‐day periods separated by a 4‐day wash‐out period. Efficacy was assessed during a period without provoking pollen in the air. At the end of each treatment period, skin‐prick tests were carried out in quadruplicate with three concentrations of grass pollen extracts (identical batches of Pharmalgen®) and histamine HCl, 1 and 10 mg/ml, as were conjunctival provocations with the same grass pollen. The mean size of weals caused by allergen and histamine was significantly smaller after terfenadine than after placebo; in fact, terfenadine increased the tolerance to the allergen by a factor of ten. Similarly, the tolerance to conjunctival provocation was significantly increased during terfenadine treatment as compared with placebo. There was no significant difference between the treatments in scores for alertness and salivation. Seasonal symptoms were mild when the children were allowed to use terfenadine in an open follow‐up study. Thus, terfenadine reduced specific as well as non‐specific reactivity in grass pollen allergic children and cau

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01979.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryAn ELISA was applied to measure IgG sub‐class antibodies to cow's milk betalactoglobulin (BLG), alpha‐lactalbumin (ALA) and alpha‐casein (AC) and to hen's egg ovalbumin (OA) in the sera of nineteen adult patients with milk intolerance causing either asthma, eczema or both. Results were compared with those of forty blood donors and twenty adult patients with either asthma or eczema due to inhalant allergy. Apart from one blood donor, high titres of IgG sub‐class antibodies to all three milk proteins were found only in the milk intolerance group. The most frequently detected antibody was AC‐specific IgG4; being high (i.e.>9·98 μg/ml) in eight milk intolerance cases: six with eczema, one with asthma and one with both. A variable proportion of these eight patients also had high levels of IgG1, IgG2 and IgG3 antibodies to AC and IgG1, IgG2, IgG3 and IgG4 antibodies to BLG and ALA. In contrast, IgG antibody to the egg protein, OA, was remarkably restricted to IgG4 and was present in high titres in 68·4% of milk intolerant patients, 60% of inhalant allergy patients and 30% of blood donors. However, the greater incidence of high titres of IgG4 antibody to OA, compared to AC, was due to the superior coating efficiency of OA resulting in a more sensitive assay. We conclude that some adult cases of milk intolerance, particularly those with eczema, can be diagnosed by detecting raised serum levels of IgG sub‐class antibodies to

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01980.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryA group of dairy farmers studied 6 years earlier in a field survey was re‐surveyed for respiratory symptoms, occupational capability and the presence of antibodies against environmental micro‐organisms. Specific IgG antibodies toMicropolyspora faeni, Thermoactinomyces vulgaris, Aspergillus umbrosusandAspergillus fumigatuswere measured by ELISA from the serum samples obtained in the two surveys. Antibody titres remained constant in 70% of both farmers and controls, but where changes took place, the titres against the actinomycete antigens tended to rise, whereas both increases and decreases were detected equally against theAspergillusantigens. The titre of specific antibody to any of the four micro‐organisms, when measured from a single serum specimen, seemed to be of little diagnostic value. Observed changes however, were more diagnostic, in that a fall in titre, especially against theAspergillusantigens, was closely associated with a definite decrease in exposure, such as after retirement. Increased titres occurred in farmers with continued exposure, and those against the actinomycetes were associated with the appearance of symptoms in previously symptom‐free individuals. In a case of farmer's lung which developed in this population during the follow‐up period, significant increases were detectable against T.vulgarisan

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01981.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SummaryTotal scrum IgE and IgE antibodies against ten common antigens, includingDermatophagoides farinae(D.f.), moth, ragweed, orchard grass, cryptomeria, alternaria, aspergillus, dog dander, cat dander and tetanus toxoid, were determined using the PRIST and the Sepharose‐RAST, respectively, in 100 clinically non‐allergic Japanese subjects and interrelations of IgE responses to these antigens were investigated. We obtained following results. (1) The number of positive RAST antigens, to which the subjects responded, increased in parallel to their total serum IgE levels. (2) Among ten antigens, D.f. and moth antigens contributed a great deal to the elevation of total serum IgE level as compared with other antigens. (3) When the relationship between IgE response to D.f. and similar responses to the other nine antigens was investigated, positive RAST responses to various antigens occurred selectively in the subjects with positive RAST for D.f., and the number of positive RAST antigens to which the subjects responded increased depending upon their D.f. specific RAST levels. (4) Such an association was not found between IgE response to moth and the other nine antigens. These findings suggest that non‐specific activation of IgE‐producing B cells occurs as a result of continuing stimulation

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1986.tb01982.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY