ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01083.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01084.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01085.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01086.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryPatients with multiple unexplained somatic symptoms attributed to allergy frequently present to physicians and often the physician fails to find evidence for allergic or immunological mechanisms underlying the presenting symptoms. This article discusses the social and cultural background to this disorder. We then consider current explanatory models for symptoms and finally, we consider appropriate management, starting with the initial consultation, the identification of psychiatric disorders when present, and concluding with suggestions for subsequent treatment and guidance on appropriate referral.

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01087.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryPrevious studies have demonstrated increased production of interleukin‐4 (IL‐4) and reduced production of interferon (IFN)‐γ in stimulated peripheral blood mononuclear cell cultures from children and adults with atopic dermatitis, however, it is unclear whether such an imbalance of cytokine production relates to other childhood atopic diseases such as asthma, and in particular to the presence of the atopic state per se. The production of IL‐4 and IFNγ in phytohaemagglutin‐ (PHA)‐stimulated peripheral blood mononuclear cell (PBMC) cultures from atopic and non‐atopic children with moderately severe chronic persistent asthma, and a group of age‐matched non‐atopic controls who did not have asthma was examined. Atopic children with asthma produced significantly more IL‐4 and less IFNγ than non‐atopic children with asthma and non‐atopic controls who did not have asthma. There was no significant difference in IL‐4 or IFNγ production between non‐atopic children with asthma and controls. These findir. 3 demonstrate that an imbalance of IL‐4 and IFNγ production is present in atopic asthma as previously documented in atopic dermatitis, therefore suggesting that it is a

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01088.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryCandida albicans46 kDa protein, a glycolytic enolase enzyme, is an important allergen of the yeast. The purpose of the study was to detect circulating IgE and IgG antibodies againstC. albicansenolase (CAE). We isolated CAE using sequential DEAE Sephacel and Pl 1 column chromatography from spheroptasts ofC. albicans, and delected IgE and IgG antibody against CAE by immunoblotting. Crossreactivity of enolose ofC. albicansandSaccharomyces cerevisiaewas also examined by immunoblotting and immunoblot inhibition test. Among 54 sera with positive IgE RAST toC. albicans, IgE antibody against CAE was detected in 20 sera (37%) and IgG antibody in 27 sera (50%). The allergenic potency of CAE was confirmed using a skin‐prick test in three patients. Simultaneous IgE binding toS. cerevisiaeenolase was only observed in four out of 20 sera reacting to CAE. Pre‐treatment of sera with CAE completely inhibited IgE binding toS. cerevisiaeenolase. Whereas the latter only partially inhibited IgE binding to CAE. These results suggest that CAE shares some crossreacting epitopes withS. cerevisiaeenolase, representing minor components of CAE but dominant segments ofS. cerevisiaeenol

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01089.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryCandida albicansenolase is one of the important allergens inCandidaallergy. We isolated and purified 46 kDa C.albicansenolase (CAE) fromC. albicansand characterized epitopes for IgE antibody by lectin‐blotting and enzymatic digestion followed by sodium dodecyl sulfale polyacrylamide gel electrophoresis (SDS‐PAGE) and immunobiotting. Lectin blotting and deglycozilation indicated that this protein did not contain polysaccharide side chains. The purified CAE and recombinant fusion protein produced from CAE gene possessed common epitopes for IgE antibody. We estimated IgE binding epitopes on the basis of reported amino acid sequences from the analysis of cDNA encoding CAE. V8 protease digestion of CAE gave six polypeptide fragments (A‐F). The N‐termini of each fragment were confirmed by amino acid sequence and the C‐termini were estimated by molecular weights of each fragment and the specific cutting site of V8 protease. Fragment C (25.0 kDa; F‐171‐I‐399) reacted to 90% IgE antibodies examined, whereas fragments D (21.0 kDa; F‐171‐1‐360), E (16.2kDa: F‐171‐D‐317) and F (13.0kDa; A‐47‐E‐170) showed no IgE binding. Our results suggest that epitopes for IgE antibodies exist n

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01090.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryThe dust miteLepidoglyphus destructoris the dominating source of allergens giving rise to asthma and rhinitis among farmers. In a previous study of the localization of allergens inL. destructorwe demonstrated that the 39 kDa allergen is associated with digestion. Here we describe the localization of the principal 15 kDa allergen and the high molecular weight allergen complex (79 and 93 kDa) inL. destructorwith confocal laser scanning microscopy (CLSM). Cryostat‐cut sections of mite bodies and faecal pellets were probed with mouse monoclonal antibodies (MoAbs) raised against the allergens. The 15 kDa allergen disclosed labelling of the mite body and most of the faecal pellets but left the exoskeleton unlabelled. The binding was widespread, and most intense in the mouth region. However, some staining was also observed around the gastrointestinal tract. In contrast, the 79 and 93 kDa allergen complex stained the exoskeleton and the front part of the mile. Interestingly, we detected no labelling of the faecal pellets with the MOAb against the 79/93 kDa allergen. The study indicates that the 15 kDa allergen is associated with the digestive tract whereas the function of the 79 and 93 kDa allergen complex remains to be elucidate

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01091.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummarySerum levels of Interleukin (IL)‐4, Interferon (IFN)‐γ and soluble CD23 (sCD23) were analysed in a prospective study of 64 infants who were monitored from birth to 18 months of age. The findings were related to family history of atopy and the development of allergic disease in the infants. Low levels of IL‐4 were detected in 10 of 63 cord blood samples (median 0.14 and range 0.32 μg/1). The levels then increased, both in healthy and atopic infants, reaching a peak at either 6 or 9 months and then decreased up to 18 months of age. The children who developed atopic disease during the first 18 months of life had significantly higher IL‐4 median levels than those who did not, i.e. 0.24 (range 0.40) vs<0.10 μg/1 at 3 months, (P<0.001), 0.40 (range 0.95) vs 0.13 (0.19) μg/1 at 6 months (P<0.01), 0.46 (range 0.78) vs 0.10 (0.24) μg/1 at 9 months (P<0.001) and 0.30 (range 1.38) vs 0.10 (0.36) μg/1 at 18 months (P<0.001). The IFNγ levels were below the detection level, i.e.<100 ng/1 in all but 49 of the 196 serum samples that were analysed. There was no significant relationship with clinical outcome, nor with S‐IgE levels. Soluble sCD23 levels increased in the infants with age. There was no association with either atopic disease, family history of allergic disease or IgE antibody levels. In conclusion, IL‐4 levels in serum, but not sCD23 and IFN7 are associated with allergic disease in infancy. Elevated levels were recorded before the onset of clinical symptoms. The findings support that atopic disease is associated with a primary abnormality

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1995.tb01092.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY