ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00945.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00946.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00947.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00948.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00949.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract.We studied total and specific serum IgE levels cross‐sectionally, potential predictors of obstructive lung disease, in a stratified random sample of 18–73‐year‐old adults (n= 1512). The attendance rate was 84%. The total IgE level and prevalences of specific IgE antibodies against house dust mite and cat were higher for men than for women. Specific IgE levels decreased by increasing age, while total IgE decreased in women only. Smokers had a higher IgE level than non‐smokers, while non‐smokers had more often specific IgE antibodies against timothy and birch than smokers. Subjects with occupational dust or gas exposure had a higher total IgE level than unexposcd. The general population prevalences were for specific IgE antibodies against timothy 4.5%, house dust mite 3.2%, birch 2.6%, cat dander 1.6% mould 0.2% and against any of these 7‐6%. In a multivariate analysis age, occupational dust or gas exposure as well as the interaction terms between sex and age and between smoking and paek‐years were independent predictors for total IgE levels. Male sex, young age, never having smoked and the season of the year were independent predictors for having one or more of the five specific IgE antibodies. Subjects with total serum IgE in the highest quintile (≥66 kU/1) had an adjusted odds ratio of 37 (95% confidence interval: 11–120) for having one or more of the specific IgE antibodies examined, compared with those in the lowest quintile (<5 kU/1). Demographic and environmental factors were thus predictors of total and specific IgE levels in this adult community. These factors should be taken into account when examining relationships between IgE levels, markers of allergy and inflammation,

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00950.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract.Hypergammaglobulinemia is a well‐known feature of liver cirrhosis, but studies on serum IgE in this setting are limited. The present study evaluates serum IgE concentration in a group of cirrhotic patients and examines their relationship with aetiological, clinical and analytical parameters (including liver function tests and hepatic phagocytic activity). The presence of specific IgE against common dietary antigens was also investigated. Total serum IgE was determined by enzyme immunoassay (EIA) in 52 cirrhotics (27 alcoholic and 25 non‐alcoholic, including eight virus B and seven virus C‐related cirrhosis, three primary biliary cirrhosis, three cryptogenic, three haemochromatosis and one Wilson's disease) and 34 healthy subjects (used as controls). Serum IgE (1U/ml) in contols was not significantly different from that of cirrhotic patients (median 42, range 2–726 vs median 86, range 2‐>1000, respectively) (P=NS). However, serum IgE among alcoholics (median 199, range 19‐<1000) was higher than that of controls (P<0‐001), virus B‐related cirrhotics (median 25, range 3‐<1000) (P<0‐05), virus C‐related cirrhotics (median 47, range 2‐170) (P<0‐05), or nonalcoholic cirrhotics as a whole (median 23, range 2‐<1000) (P<0.01). High IgE levels (≥ 170 1U/ml) were detected in 55.5% of alcoholics compared with only 12% of nonalcoholic cirrhotics (P1000 1U/ml) in six patients of the alcoholic group (22.2%) compared with only one non‐alcoholic patient (4%). Increased serum IgE levels did not seem to be related to either parameters of liver function (serum bilirubin, albumin, prothrombin or Child‐Pugh score), hepatic phagocytic function or other immunoglobulins. Skin‐prick tests failed to demonstrate increased sensitization to eight common dietary antigens in the patients studied. On multivariate analysis, only the alcoholic aetiology of the disease correlated with high serum IgE levels. These data suggest that increased serum IgE might be a common and rather specific feature

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00951.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract.To investigate the role of cereal α.and β‐amylase in bakers’ asthma, we have compared the IgE response of 30 wheat‐flour‐allergic individuals to barley α and β‐amylases with that of fungal α‐amylase using radioallergosorbenl test (RAST), RAST inhibition assays and Western blotting. RAST analysis showed 29 of the 30 subjects with inhalant induced cereal allergy had positive IgE to cereal amylases, but only 16 were positive to fungal α‐amylase. Regression analysis showed an association between specific IgE to wheat‐flour and to barley α‐amylase (r =0.70) and barley β‐amylase (r= 0.92) but a poor association with fungal α‐amylase (r = 0–34). RAST inhibition showed minimal crossreactivity between barley α or β‐amylase and barley and fungal α‐amylase. Sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and Western blotting showed that non‐reduced barley α‐amylase had a molecular weight of 54 kDa and barley β‐amylase a molecular weight of 64 kDa. Reduced fungal α‐amylase had a molecular weight of 54 k Da. Cereal α and β‐amylase appear t

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00952.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract.Studies employing sera from 34 subjects allergic to while cypress pine (Callitris glaucophvlla) pollen identified 18 IgE antibody‐binding components in the pollen of this species, five of which (MWs∼94, 68, 64, 43 and 34 kDa) were recognized by all of the sera. Protein blotting and quantitative inhibition studies revealed clear cross‐reactivity between C.glaucophyllaandCupressus sempervirenspollen proteins and striking similarities in the IgE recognition band patterns of the two pollens. Inhibition experiments with other pollen extracts revealed that sera from C.glaucophyllapollen‐allergic subjects can be divided into two groups – those inhibited only by extracts from the two Cupressaceae pollens and those inhibited both by these pollen proteins and by pollen extracts from other species. Most of the crossreactions in the latter group cannot be explained on the basis of taxonomic relationships or separate sensitizations. As with previous studies on birch and olive pollens, we conclude that pollen allergenic crossreactivity is much more wide‐ranging than general

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00953.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract.Airborne pollen grains und small plant particles of respirable size are the main causes of rhinitis and asthma in pollinosis patients. Consequently, it could be useful to quantify atmospheric variations in these biological aerosols and their allergenic activity as a basis for establishing correlations with the clinical symptoms in these allergic subjects. Our study was conducted in Naples from May to August 1992 and from May to August 1993 and concentrated onPerietaria judaica (Par j) the most important hay fever‐provoking plant in southern Italy. The aim of this study was lo evaluate the day‐to‐day variation in counts ofPar j.pollen, the day‐to‐day variation in allergenic activity specific for this plant, and to relate these two variables to symptom scores in patients with respiratory allergy toPar j.The airborne pollen grains were collected by a Hirst‐like volumetric trap and examined through an optical microscope, while the allergenic activity of particles trapped on glass fibre filters in a high volume air‐sampler was determined by immunochemical assay. The two devices were placed on the flat roof of the “A. Cardarelli” Hospital. The results of this preliminary study suggest that both methods could have useful clinical relevance, since these two biological parameters were significantly correlated with the symptom scores of allergic patients. However, pollen count with morphological quantification of grains/m3of air is a more

ISSN:0954-7894    

DOI:10.1111/j.1365-2222.1994.tb00954.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY