摘要:

Intra‐uterine injection of the lectin Concanavalin A (ConA) on day 5 of pseudopregnancy induced a rapid and persistent infiltration of leucocytes into the rat uterine stroma. Although the infiltration of leucocytes was seen along the entire length of the uterine horn, areas of stromal oedema, indicative of decidualization (as indicated by a positive Pontamine Sky Blue reaction), were only associated with regions in which leucocytes had crossed the uterine epithelium and were present in the uterine lumen. Ultrastructural evaluation of the interaction of the luminal leucocytes with the apical surface of the uterine epithelium appeared strikingly similar to that of the blastocyst and the uterine epithelium during normal implantation. It is proposed that leucocytes, induced by ConA, may initiate a decidual response in a manner analogous to that of the blastocyst through surface epithelial interactio

ISSN:1065-6995    

DOI:10.1006/cbir.1996.0093

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Glycoproteins are thought to play a crucial role in cell—cell interactions during nervous system ontogeny both in vertebrates and invertebrates. In order to investigate the putative involvement of such molecules during bee brain ontogeny we used lectins for their ability to bind specifically carbohydrate moieties. The expression of four lectin receptors, i.e.Arachis hypogea(PNA),Triticum vulgaris(WGA),Glycine max(SBA), andConcanavalin A(Con A) has been studied during pupal development and in the adult. The antennal lobe shows a complementary pattern of expression of Con A which stains both neuron somata and glomerular contours, and PNA, which stains the glomerular neuropile. SBA strongly stained the perineurium, trachea and mushroom body neuropile, while other neuropiles were not stained. WGA stained neuronal somata and the core of the glomerul

ISSN:1065-6995    

DOI:10.1006/cbir.1996.0095

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Exchange of erythrocyte intracellular (i/c) K+for extracellular (e/c) Na+in human erythrocytes treated with sub‐CMC concentrations of the non‐ionic detergent Brij 58 can be stopped by reincubation in serum or albumin containing solutions. The progressive equilibration of the K+contents of detergent‐treated human erythrocytes with the incubation medium was reversed by an albumin‐mediated withdrawal of detergent molecules from the cell. Re‐establishment of near normal [K+] in terms of K+/kg water proceeds in two ways: (i) a metabolism‐dependent net accumulation of K+ions; and (ii) a metabolism‐independent shrinkage of erythrocytes, this being the more signi

ISSN:1065-6995    

DOI:10.1006/cbir.1996.0096

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

It was previously shown that during the acute‐phase response‐induced elevated transcription of the rat haptoglobin gene, protein p55 and the lamins mediate the increased binding of restriction fragment II (−541/−146) via a 38 bp adenine tract lying 147bp upstream from the haptoglobin gene cis element (−165/−56), to scaffold type II‐like nuclear matrices. Here we show that the fragment II binding pattern to ≥40 kDa proteins of nuclear matrices analogous to type I scaffolds is more complex. Fragment II bound conspicuously to a 40 and less so to p55, a 60 kDa protein and the lamins of control matrices. During increased gene transcription, it bound prominently to p55, the lamins, and less so to 45 and 52 kDa proteins. This was accompanied by the tenacious binding of the DNA to isolated nuclear matricesin vitroand post‐translational modifications of certain matrix proteins. The lamins and p55 demonstrated a greater N‐acetylglucosamine/sialic acid content and p55 an increasedin vitrophosphorylation by a nuclear matrix‐associated cyclic‐nucleotide‐independent kinase. The acute‐phase response also caused an increased partitioning of p55 with the nuclear matrix. It was concluded that, as a result of a molecular remodelling of the nuclear matrix at the point of contact with chromatin, the nature of its association with region II DNA changed during elevated

ISSN:1065-6995    

DOI:10.1006/cbir.1996.0097

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Apoptosis was measured in mammary glands during whole organ culture, to determine whether regression resulting from hormone withdrawal results in epithelial cell death asin vivoinvolution. Glands were evaluated for morphology and DNA degradation prior to whole organ culture, after lobulo‐alveolar development and 2, 4, or 6 days after hormone withdrawal. The data indicated that mammary regression during whole organ culture mimicsin vivoinvolution and results in part from apoptosis of epithelial tissu

ISSN:1065-6995    

DOI:10.1006/cbir.1996.0098

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The anti‐desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X‐100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were searched for in an IFs‐deficient clone of SW13 cells. Reactive materials specific to DSB389, AC54, and DSB860 MAbs were trapped at the top of the gel of SDS—agarose—PAGE. The reactivity of the materials disappeared after treatment with DNase I. The reactivity, or trapping of this material at the top of the gel, required previous heat treatment of the sample before application to the gel. The MAbs recognized both single‐stranded and double‐stranded DNA in ELISA. These results indicate that at least the main nuclear antigens of DSB389, DSB860 and AC54

ISSN:1065-6995    

DOI:10.1006/cbir.1996.0099

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

A recombinant baculovirus was constructed to express a cDNA encoding RelA (p65), a member of the NF‐κB/Rel family of proteins. Infection ofSpodoptera frugiderdainsect cells with the recombinant baculovirus resulted in the production of the biologically active protein as measured by immunoblotting using RelA‐specific antisera and by electrophoretic mobility shift assays. The recombinant protein bound specifically to an oligonucleotide containing the NF‐κB consensus motif but not to that containing the unrelated Oct‐1 consensus motif. Thus insect cell‐derived RelA possess properties similar to the native protein and may be used in physical, biochemical, and pharmacologi

ISSN:1065-6995    

DOI:10.1006/cbir.1996.0100

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY