ISSN:1065-6995    

DOI:10.1006/cbir.1995.1035

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1036

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

AbstractTranscriptional regulation and binding interactions between soluble nucleoproteins and the hormone response elements (REs) of the rat haptoglobin (Hp) and α2‐macroglobulin (α2MG) genes was examined in the livers of rats during the acute‐phase reaction. Our results demonstrate that the elevation of the Hp and α2MG genes' transcription rates in acute‐phase liver relies essentially on an increase in the binding‐affinity of pre‐xisting soluble nucleoproteins, enhancing their capability to bind the examined cis‐regulatory elements. The 35kD nucleoprotein that displayed an acute‐phase inducible affinity to bind hormone REs of rat Hp and α2MG genes, was identified as

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1037

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

AbstractWith confocal microscopy it is possible to study the Concanavalin A (Con A) binding characteristics of the surface and interior of a single cell by viewing optical sections. It was observed in Tetrahymena pyriformis that Con A bound both to the plasma membrane and to intracellular structures. Incubation of cells with a competing sugar a‐methylmannopyranoside, decreased binding. Hormonal imprinting with insulin resulted in an increase in binding of Con A to the cell surface and a decrease in intracellular binding. It is possible that the intracellular binding sites may migrate to the plasma membran

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1038

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

AbstractMineralization occurred both in fetal rat calvarial cells and UMR 106 osteoblastic cells when they were cultured in medium containing L‐ascorbate and β‐glycerophosphate as evidenced by von Kóssa staining as well as deposition of calcium ions and inorganic phosphate in the cells. When compared with corresponding non‐mineralized cell cultures, both the mineralized cultures of calvarial cells and UMR 106 cells did not exhibit any change in intracellular bone‐specific alkaline phosphatase activities which were measured by wheatgerm lectin precipitation method. Our results support the hypothesis that mineralization may not exert any direct negative feedback on matrix protein synthesis in osteoblasts during bone

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1039

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

AbstractErythrocytes isolated from 131 cases of Non‐Insulin Dependent Diabetes Mellitus (NIDDM) were studied for lipid peroxidation, antioxidant defences, and the maximum peroxidisable substrate in the cell membrane. Antioxidant defences are lowered in NIDDM, followed by significant rise in lipid peroxidation products. However, in the erythrocyte membrane, the total polyunsaturated peroxidisable lipids are lower than in normal erythrocytes which may be a causative factor affecting the survival of the cell

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1040

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

AbstractThe genome size of the marine spongesSuberites domunculaandGeodia cydoniumhas been determined by flow cytofluorometric analysis using diamidino‐phenylindole [DAPI]. Using human lymphocytes as reference the amount of DNA in cells fromS. domunculahas been determined to be 3.7 pg and that ofG. cydonium3.3 pg. While no chromosomes could be identified inG. cydonium, the karyotype of theSuberites domunculais 32 chromsomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 μm. No pronounced banding pattern was visib

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1041

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

AbstractThe rat pericoronary adipose tissue was perfused in the presence of either the liposynthetic hormone insulin or the lipolytic hormone noradrenaline. Insulin perfusion associated with a) larger adipocyte mean sectional diameter in comparison with noradrenaline perfusion; b) glycogen deposition; c) appearance of small fat globules at discrete sites at the periphery of the main lipid drop. The two latter phenomena were apparently dose‐dependent. Massive lipid deposition was induced by addition of triglycerides to the perfusion medium and this associated with appearance of prominent endoplasmic reticulum in the cytoplasm. In noradrenaline‐perfused adipose tissue many small lipid droplets surrounded the central lipid deposit and the endoplasmic reticulum was in the form of both thin long, dashed cisternae sometime surrounding lipid droplets and grouped, anastomosing tubular cisternae. The present work shows that the perfused white adipose tissue of the heart is a suitable model to study, in situ, the morphological effects of hormones in adipocy

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1042

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

AbstractInsulin treatment of Tetrahymena pyriformis resulted in a long‐lasting increase in the insulin content of the cells. After about 200 generations, insulin levels significantly higher than in the control were demonstrated by using a quantitative immunocytochemical method. Although the insulin content fluctuated from day to day, it was always higher in the insulin pre‐treated (imprinted) cells than in the controls. The results emphasise the simultaneous stimulation of hormone and receptor formation by hormonal imprint

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1043

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

AbstractAdhesion of cells to biomaterial surfaces is one of the major factors which mediates their biocompatibility. Quantitative or qualitative cell adhesion measurements would be useful for screening new implant materials. Microjet impingement has been evaluated by scanning electron microscopy, to determine to what extent it measures cell adhesion. The shear forces of the impingement, on the materials tested here, are seen to be greater than the cohesive strength of the cells in the impinged area, causing their rupture. The cell bodies are removed during impingement, leaving the sites of adhesion and other cellular material behind. Thus the method is shown not to provide quantification of cell adhesion forces for the metals and culture plastic tested. It is suggested that with highly adherent biomaterials, the distribution and patterns of these adhesion sites could be used for qualitative comparisons for screening of implant surfaces.

ISSN:1065-6995    

DOI:10.1006/cbir.1995.1044

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY