摘要:

The immunoperoxidase technique was used to study the occurrence of placenta‐associated plasma protein A (PAPP‐A, SP4) in formalin‐fixed paraffin‐embedded tissue specimens from 15 hydatidiform moles, 6 invasive moles and 7 choriocarcinomas. PAPP‐A was found in the syncytiotrophoblast of all hydatidiform moles, in 5 of the 6 invasive moles and in 6 of the 7 choriocarcinomata. No clinical difference was observed between PAPP‐A positive and negative cases of invas

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00189.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

A microspectrophotometric study of the DNA content of carcinoma‐in‐situ germ cells in the testes of eight infertile men revealed an aneuploid DNA distribution pattern. The results support the assumption that carcinoma‐in‐situ germ cells are of a malignant nature. The findings also indicate that the DNA content can be used to discriminate between carcinoma‐in‐situ germ cells and sp

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00190.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Both reendothelialized and non‐reendothelialized surfaces of rabbit aortas, subjected to an embolectomy catheter lesion, were sectioned parallel to the neointimal surface. Microfilament bundles within endothelial cells were associated with extracellular anchoring filaments, lying parallel to the long axis in sections from reendothelialized areas. Proteoglycans coated the surface of anchoring filaments and joined together all the elements of connective tissue in the subendothelial space. In non‐reendothelialized areas covered by smooth muscle cells, anchoring filaments were less prominent, almost no proteoglycans were visible, and connective tissue components were randomly mixed. We propose an influence of endothelial cells on the orientation and composition of the subendothelial connective tis

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00191.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

A commercially available cell line (NCTC 2544) originating from presumably normal human skin was chosen as an in vitro model system for subsequent studies of the effects of different agents on human epithelial cells. The cell line, therefore, was ultrastructurally and otherwise characterized at intervals by techniques which allow standardized controls of the model. The cell line was classified as epithelial both by phase contrast microscopy and transmission electron microscopy. The cells were polygonal, fully developed desmosomes were demonstrated and no extracellular filamentous material was observed. Specific epidermal markers like keratohyalin granules or keratinosomes were not demonstrated. Comparison of the nuclear morphology of cells from 1, 3 and 6 days old cultures revealed that only minor changes took place. The same was true for cytoplasmic features. Thus this cell line has a well‐defined ultrastructural morphology. Flow cytometry studies showed the cell line to be tetraploid, with no sub‐populations with other ploidies. Surface antigens were typed by a microcytotoxicity assay using 12 different antisera. The electrophoretic patterns of the isoenzymes lactate dehydrogenase and glutathione reductase were human. The cells did not form colonies in soft agar. Though several parameters indicate that this cell line is not a HeLa cell contaminant, this possibility cannot, however, be totally exclu

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00192.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

An established human epithelial cell line was exposed to photoactivated 8‐methoxy psoralen (PUVA); the cells were fixed and processed for electron microscopy 2 hours, 3 days and 6 days thereafter, and the nuclear morphology compared to controls. In the light microscope the cells fixed 3 and 6 days after PUVA exposure showed an increase in the number of multinucleated cells. At the ultrastructural level an increased number of cells with nuclear indentations, cytoplasmic invaginations, pseudoinclusions, nuclear bodies and multiple nucleoli were seen. An increased number of perichromatin granules per cell section and a decrease in heterochromatin situated marginally were observed. In cells from 6 day‐old cultures 15 out of 50 nuclei were seen divided into 3–12 nuclear elements, compared with none in the controls. The smaller of these elements tended to be heterochromatic and the larger euchromatic. Generally the nuclear elements were smooth in outline, but elements of bizarre shape were also seen. These consisted of nuclear fragments united by heterochromatic extensions of uniform diameter. When comparing the morphology of PUVA exposed cells with the morphology of non‐malignant and malignant cell lines, the PUVA exposed cells appeared to show an increase in morphological markers corresponding to those determined in malignant cells. The possibility that PUVA therapy may cause cancerous transformation is di

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00193.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Eighty‐three non‐Hodgkin lymphomas classified according to the Kiel classification have been studied with regard to surface immunoglobulin (slg) on cells in suspension and cytoplasmic immunoglobulin (clg) by the peroxidase anti‐peroxidase method (PAP) on formaline‐fixed tissue sections. Fifty‐six out of 66 examined (i.e. 85%) revealed a monoclonal staining pattern for slg, whereas 37/70 (53%) gave a monoclonal staining pattern for clg by PAP. The methods combined gave a monoclonal staining pattern in 73/83, i.e. in 88%, of the biopsies tested. The discrepancies between the two methods were largest in centroblastic/centrocytic and lymphocytic lymphomas. With regard to the light chain staining patterns, complete agreement between the two methods was obtained in the 20 cases that allowed such analysis to be made. This suggests that the specificity of PAP, as carried out in this study with reagents purified by immunoabsorbent techniques, is satisfactory. On a basis of heavy chain isotypes centroblastic/centrocytic, lymphoplasmacytoid, and immunoblastic lymphomas could be divided into distinct immunological subgroups. In four biopsies the slg heavy chains were μ+δ, whereas μ+γ chains were detected by PAP. This finding may be relevant to the μγ switch known to occur during normal B‐cell differentiation. Immunoglobulin inclusions were found in 8 cases ‐ 3 belonging to the immunoblastic group, and 5 to the lymph

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00194.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

The application of fine needle aspiration biopsy cytology to tumor diagnosis and classification is gaining increased acceptance. The classification of some poorly differentiated tumors may prove particularly difficult for the cytologist as well as for the pathologist. In such cases electron microscopic examination of fine needle aspiration biopsy material can be of great assistance. A simple, yet dependable, technique is described for the study of such material. The method has been shown to be of decisive importance in the classification of 80% of a selected series of cases submitted to ultrastructural analysis. Using this method, a preoperative diagnosis can be obtained in otherwise equivocal cases.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00195.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Repeated injections of small doses of streptozotocin (s.z.) lead to a slowly‐developing hyperglycemia with a concomitant infiltration of lymphocytes into the pancreatic islets. Similarly, intrasplenically islet‐implanted but otherwise normal mice became diabetic when given the multi‐s.z.‐treatment, suggesting that the splenic location of the islets does not protect them from the toxic effects of s.z. or immunereactions. Intrasplenic, syngeneic islet implantation normalized the elevated blood sugars of multi‐s.z: treated mice, irrespective of whether the transplantation was performed 8 or 15 days after the first injection of s.z. These findings suggest that s.z. has to be present in order to trigger the auto‐immune process of this insulitis model. In support of this suggestion, we observed that hyperglycemic, multi‐s.z.‐treated mice cured by islet implantation reverted to a hyperglycemic state when treated repeatedly with small doses of s.z., suggesting the presence of a booster

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00196.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Thein vitrosystem was used to study early ultrastructural effects following gamma irradiation of human pituitary adenomas and neurinomas with 30 and 70 Gy single doses, respectively. The acoustic neurinoma cells showed a large number of intracellular myelin figures and vesiculation of cytoplasm. The pituitary tumour cells showed vacuolization of cytoplasm and cell organelles. Occasionally, electron‐dense cytoplasmic inclusion bodies with a fibrillar substructure occurred. The differences between the two types of tumours in irradiation‐induced morphological changes may indicate differences in their biochemical structure and metabolic activities. The morphological findings after irradiation of specimens from all four neurinomas were rather similar after the same length of time but the structural alterations of pituitary cells differed considerably between individual tumours. The 70 Gy dose caused more rapidly‐occurring morphological alterations than the 30 Gy single

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00197.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

A 16‐year‐old girl presented with a left‐sided abdominal mass. X‐ray examination and computed tomography disclosed a well‐defined tumor close to the tail of the pancreas, stretching the pancreatic branches from the splenic artery as demonstrated by arteriography. Ultrasonography suggested a partly cystic character. Cytologic fine‐needle biopsy, histopathological and electron microscopical examination disclosed a cysto‐papillary tumor, probably benign, and of an exocrine, ductular origin, and with pseudo‐cystic areas similar to those found in adenoid cystic carcinoma of salivary glands. Immunohistochemistry did not demonstrate the presence of polypeptide hormones. The patient is without signs of tumor recurrence 18 months

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1981.tb00198.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY