摘要:

Ultrastructural changes in cell nuclei were studied in kidney biopsies from 18 patients suffering from Nephropathia epidemica. Three patients showed small nuclear particles in a large aggregate in a few cells of the distal tubule. The particles had a diameter of about 30 nm and their occurrence was not associated with intravenous glucose infusions. Large light nuclear bodies were numerous in the nuclei of both interstitial and tubular cells. Many showed strands of darker material about 35 nm in diameter at their center. Nuclear vesicles with varying amounts of membrane debris were seen in both tubular and interstitial cells. Occasional interstitial lymphocytes showed abnormal margination of chromatin. Of the changes observed the small nuclear particles are not found in kidney biopsies in other conditions and it is possible that they are aggregates of viral protein.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00026.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The influence of 4‐aminopyrazolopyrimidine (4‐APP) on morphology and on synthesis and secretion ability of isolated rat hepatocytes was investigated: 4‐APP was found to inhibit both the synthesis and secretion of proteins. The synthesis of triglycerides was unaffected by 4‐APP, while the secretion of triglycerides was markedly reduced. Transmission electron microscopy revealed that 4‐APP induced morphological changes in the smooth membrane systems, the smooth endoplasmic reticulum and the Golgi apparatus. The possibility that 4‐APP inhibits lipoprotein formation by inhibition of the apoprotein synthesis i

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00027.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A light microscopical study of an excised testis from a 14 1/2‐year‐old girl with incomplete type of testicular feminization syndrome revealed a carcinoma‐in‐situ pattern in a part of the gonad. An identical histological pattern has repeatedly been demonstrated in infertile men who developed gross testicular germ cell tumours. It is suggested that germ cell carcinomas in patients with testicular feminization syndrome may be preceded by this characteristic intratubular germ cell abno

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00028.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The densities of human parathyroid glands were measured in density gradient columns of various media. Percoll (an aqueous colloidal solution of polyvinylpyrrolidone‐coated silica), equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density>1.0 g/ml, which includes most parathyroid glands. The densities of the glands varied between 0.96 and 1.06 g/ml. Density measurements in gradients of Percoll were simple and reproducible and were made with an accuracy of approximately 0.001 g/ml. In other gradient media (aqueous solutions of sucrose and Ficoll, and organic solvents) there was a drift in density caused by osmotic or lipid‐solving effects of the media. For measurements of densities<1.0 g/ml no ideal gradient medium was found, but a silicon oil or carbon tetrachloride/kerosene gradient could be used with somewhat reduced accuracy. It is concluded that the density gradient technique with the use of Percoll is potentially useful as a complement to routine histopathological parathyroid diagnosis, as glandular density is a good indicator of the relative proportions of parenchymal and fat tis

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00029.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

We have studied four syngeneic murine tumors, a fibrosarcoma, a squamous cell carcinoma and their two ascites‐converted counterparts, by transmission electron microscopy. The ascites tumors were investigated both as cell suspension (AA‐tumor) and as solid tumor (AS‐tumor). We have also studied the ascites tumors, enzymatically produced cell suspensions of the AS‐tumors and solid original tumors by scanning electron microscopy. The two ascites tumors are totally de‐differentiated and lack intercellular junctions. This, we think, is a prerequisite for ascites growth. But the ascites tumors show no peculiar ultrastructural features in comparison with other undifferentiated malignant tumors. We found no morphological cell alterations by the enzymatical dissociation procedure used to bring the solid tumors into suspension. Differences between the AS and AA tumors can be ascribed to differences in proliferat

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00030.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

An ultrastructural study of 10 liposarcomas is reported. Four of the liposarcomas were wholly or predominantly of well‐differentiated, lipoma‐like or fibrosing type, 3 of myxoid type, 2 of round cell type and 1 of pleomorphic type. The well‐differentiated, lipoma‐like liposarcomas showed cells with a few, large lipid droplets, few organelles and a peripherally located, fairly large nucleus. The well‐differentiated liposarcomas of fibrosing type revealed mostly spindle‐shaped, fibroblast‐like cells, with abundant rough endoplasmic reticulum and inconspicuous lipid inclusions, surrounded by collagen. One well‐differentiated liposarcoma contained an area which was similar to brown adipose tissue and hibernoma. The spindle and stellate shaped cells of the myxoid liposarcomas showed abundant rough endoplasmic reticulum and large smooth‐membraned vacuoles filled with moderately dense amorphous material, which appeared to be extruded extracellularly by rupture of the vacuoles. Cytoplasmic lipid droplets were seen in most cells but were much less prominent than in the well‐differentiated lipoma‐like liposarcomas. Ultrastructurally there were many similarities between the myxoid and round cell liposarcoma, indicating a close relationship between the two types. The pleomorphic liposarcoma revealed cells with one or more large, irregular nuclei, numerous large vacuoles after dissolved lipids, abundant dilated cisternae of rough endoplasmic reticulum and rounded, electron‐dense bodies corresponding to PAS‐positive hyalin globules seen in the light microscope. The ultrastructural study suggests that the variegated cellular appearance of the different subtypes of liposarcoma reflects the wide cellular spectrum seen during the differentiation of adipose tissue and supports the view that al liposarcomas histogenetically

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00031.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A randomized morphometric study of »spontaneous« endothelial cell injury in rabbit aorta was performed blindly. Four different areas of the aorta were sampled in a standardized fashion and examined by scanning electron microscopy. The finding of protruding endothelial cells was taken as sign of injury. The endothelial cell injury was focal and, in the sampled areas, particularly prevalent in the distal part of the lesser curvature of the aortic arch and the distal lip of intercostal artery orifices. In the area between two intercostal artery orifices on the same side of the midline injured cells were only exceptionally found. Rabbits fed a 2% cholesterol diet for 2 days showed an increase in protruding cells, particularly at the intercostal artery orifices. It is concluded that the endothelial cell injury in rabbit aorta may be caused by local factors, determining the focal nature, and by systemic factors, such as acute hypercholesterolemi

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00032.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A selected series of 669 primary cutaneous malignant melanomas, stage I, was studied. The series includes 86 lentigo maligna melanomas, 259 superficial spreading malignant melanomas, 194 nodular malignant melanomas and 130 unclassifiable malignant melanomas. The cross‐sectional profile, level of invasion, ulceration and vascular invasion were graded. The relation of these features to each other and to tumour type was studied by X2tests. The prognostic value was also studied. The most common finding was a slightly elevated surface, level III of invasion, no ulceration and no vascular invasion. Most of these tumours were superficial spreading malignant melanomas. A good prognosis was associated with a flat cross‐sectional profile, level II of invasion, no ulceration and no vascular invasion. A poor prognosis was associated with marked protrusion of the surface, level IV–V of invasion, ulceration and vascular invasion. Lentigo maligna melanomas tended to be more benign while nodular malignant melanomas tended to be more malignant than the average. A superficial spreading malignant melanoma could vary in either direction. The prognostic value of level of invasion and ulceration was found to be greater than that of tumour type. The prognostic importance of invasion no further than level III is stressed. Level of invasion ought to be reported to the clinician as well as the tumour

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00033.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Testicular and liver biopsies were obtained from thirthy consecutive patients at the age of 30 to 64 years with chronic alcoholism (daily consumption of 72 g of alcohol or more for at least five years). The spermatogenesis was normal in 12 patients (40 per cent), moderately reduced in 15 patients (50 per cent) and severely reduced in 3 patients (10 per cent). No relation between the presence of liver disease as assessed by histological examination of the liver biopsy and the impairment in the spermatogenesis could be demonstrated.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00034.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Skin tumours were induced in female Wistar SPF‐rats by different polycyclic aromatic hydrocarbons, benzo[α]pyrene (BP), dibenz[α, h]anthracene (DBA), 7,12‐dimethylbenz[α]anthracene (DMBA), and 3‐methylcholanthrene (MCA) being applied on the dorsal skin of 20 rats each. DBA was not carcinogenic under the experimental conditions. DMBA proved the most potent carcinogen, and MCA was more potent than BP. Half of the animals in each group underwent skin‐surface lipid extraction (SSLE) before the application of carcinogen. SSLE did not influence the cumulative number of rats with skin tumours during an observation period of 15 months nor the type of tumours induced. The lipid extraction, however, increased the latency period and decreased the rate of tumour development when BP and MCA acted as carcinogens. On the contrary, SSLE enhanced the rate of tumour production by DMBA and reduced the latency period. The role of sebum and its composition in skin carcinogenesis is discussed, and an explanation of the different influence of SSLE on BP and MCA carcinogenesis in contrast to DMBA carcinogenesis is sought in differences in metabolic activation of the c

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1979.tb00035.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY