摘要:

The pattern of intimal folds was studied around intercostal artery orifices in rabbit aorta by scanning electron microscopy and light microscopy. The area just proximal and distal to the orifices revealed a smooth surface lacking folds, reflected by a stretched internal elastic membrane which showed no undulation. Lateral as well as further proximal and distal well‐preserved folds were observed. It is reasonable to suppose that the pattern of intimal folds in this area reflects the special flow conditions prevailing in and at the intercostal artery orifice

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02468.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

43 renal fine‐needles aspirates, obtained with a modified sampling technique were subjected to ultrastructural examination with the aim of assessing the diagnostic value of this method in various glomerular diseases. Findings in the aspiration specimens, consisting of solitary glomeruli, were regarded as diagnostic in 35% of the cases. When considered together with clinical data, the fine‐needle aspiration biopsies were suggestive of diagnosis or provided valuable information in a further 49% of the cases. Due to the small outer diameter of the fine‐needle, 0.7 mm, complications following this kind of biopsy are practically negligible. Thus, it offers a safe alternative to the conventional renal biopsy methods utilizing large‐bored cutting needles in cases where biopsies with these methods are contrain

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02469.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Quantitative histomorphometric analyses of iliac crest biopsy specimens were performed after tetracycline double‐labeling in 41 normal individuals, 20 hyper‐ and 10 hypothyroid patients, 18 patients with primary hyperparathyroidism, 20 epileptic patients receiving long‐term anticonvulsant therapy, and 17 patients after jejunoileal bypass for morbid obesity. The mineralization lag time in trabecular bone or the period of time between apposition and subsequent mineralization of osteoid was calculated from the bone formation rate at BMU level (Basic Multicellular Unit) and the mean width of osteoid seams. The mineralization lag time was 8–52 days (median 21 days) in normal individuals and showed no variation with sex or age. The mineralization lag time was shortened in hyperthyroidism, normal in anticonvulsant bone disease and in primary hyperparathyroidism, and markedly prolonged in hypothyroidism and following jejunoileal bypass. Among all individuals an inverse hyperbolic relation (r = 0.94, p<0.001) was found between the mineralization lag time and the average cellular activity of the oste

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02470.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Nineteen cases of xanthogranulomatous pyelonephritis (XP) were studied. There was a close relation to pyonephrosis, and urinary tract obstruction was present in all cases but one which represented the tumorous variety of XP. The degree of xanthogranulomatous involvement was correlated to the severity of the acute inflammatory affection as evidenced by the clinical findings and the urographical excretory function. The topography of the xanthogranulomas was related to the level of urinary tract obstruction in accordance with the pattern of reaction common to obstructive pyelonephritis. Haemorrhage and considerable fibrous thickening and stenosis of arteries surrounding the xanthogranulomatous foci were conspicious features. Based upon a morphological staging it was shown that the structural changes (towards fibrosis) were parallelled by a series of cellular events indicating removal of intracellular lipids in xanthoma cells by transformation to lipofuscin and transport in PAS‐positive interstitial macrophages. This was comparable to the findings in experimental XP suggesting a similar and simple way of pathogenesis. It is concluded that XP — with the possible exception of the tumorous variety — can be ascribed to local resorption phenomena caused by concurrent factors usually involved in pyelonephritis, urinary tract obstruction being the essential precondition, focal haemorrhage and ischaemia contributary ele

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02471.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

A morphometric study of 49 randomly selected human aortas is reported with regard to the distribution of atherosclerotic lesions in the descending thoracic aorta (DTA). The intercostal artery area (ICA‐area) revealed a higher degree of atherosclerosis compared with the remaining part of the DTA. The difference was more striking at overall lower grades of atherosclerosis of the DTA. The ventral aspect of the DTA in the proximal and caudal thirds showed a higher degree of atherosclerosis compared with the middle third. The atherosclerotic indices of the proximal and distal areas correlated significantly with increasing heart weights but not for the middle third. The explanation of this is, presumably, irregular flow conditions. For the distal part a possible effect of continuous pounding of an enlarged heart upon the ventral aspect of the descending thoracic aorta is discusse

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02472.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

In a histopathological and immunocytochemical study of biopsy and/or operation specimens from 27 patients with endocrine tumors of the colon and rectum (»hind‐gut carcinoids«) enkephalin‐immunoreactive tumor cells were observed in two cases. Both patients were obese women, about 50 years of age, with a history of constipation. The tumors were situated near the anus in the dorsal wall of the rectum. One tumor had metastasized to a lymph node, and the other showed vascular invasion. The tumor cells were non‐argentaffin; some were argyrophil. One tumor contained only few enkephalinimmunoreactive cells but had numerous β‐endorphin‐immunoreactive cells, which were distinct from the former. The other contained large numbers of enkephalin‐immunoreactive cells but no β‐endorphin cells. Both tumors also harboured glucagon‐immunoreactive cells; in one there were also cells containing immunoreactive pancreatic polypeptide. These cells were distinct from the enkephalin‐storing ones. No 5‐hydroxytryptamine could be det

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02473.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Grafts from a methylcholanthrene induced mouse uterine cervical carcinoma (NMRI mice) were grown in neonatal female mouse hosts. The hosts were treated for 12 days with estradiol and progesterone alone or in combination. Controls were injected with the solvent (olive oil) only. Pieces from every graft were used for histological study, for study of the proliferation rate and for study of the adenylate cyclase activity in homogenate. All the grafts were well differentiated squamous carcinomas and the histological picture was the same irrespective of hormonal treatment. The proliferation rate was the same in control grafts as in those from hosts treated with hormones. Grafts from progesterone and progesterone/estradiol injected hosts had significantly higher levels of adenylate cyclase activity compared with grafts from control hosts. Estradiol alone resulted in a trend towards increased activity but the difference to controls was not statistically significant.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02474.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

In suspension cultures of JB‐1‐E cells approximately 11% of the cells contained a single large vacuole (»balloon cells«) or signet‐ring cells). Such cells were rarely observed in monolayer cultures, where the cells were attached to a substrate. Only a few »balloon cells« were observed in suspension cultures of HeLa S3 cells. Both cell lines were able to phagocytize latex particles of 5.7 μm mean diameter. Nocodazole significantly reduced the formation of »balloon cells« in suspension cultures of JB‐1‐E cells. Transmission electron microscopy indicated that various cytoplasmic membrane components probably could contribute to the membrane material of the vacuoles. It is believed that the formation of »balloon cells« is due to changes in the regulation of the cellular

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02475.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

A silver‐gold impregnation technique for demonstration of cross striations in skeletal muscles is presented. It is easily performed and all ingredients are chemically well characterized and easily available. The method has been successfully applied to formalin‐fixed paraffin‐embedded material from human muscles and can be of value in the histopathological diagnosis of rhabdomyosarcomas and other tumors of muscular o

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02476.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Sera from patients with active chronic hepatitis were used to demonstrate actin microfilaments in cells of a bone‐induced model. Demineralized and freeze‐dried allogenic dentine was implanted in the abdominal muscles of Guinea pigs. After 21 and 28 days the tissue was excised and showed: a) resorption areas of the dentine contained dentinoclasts and macrophages. b) osteoid and bone and c) areas of fibroblastic proliferation. Osteoblasts and young osteocytes showed the strongest fluorescent staining. Electron microscopy of these cells demonstrated abundant 5–7 nm microfilaments interpreted as actin in the outer parts and cell projections, in which some microtubules also were observed. Fewer microfilaments were present in mature osteocytes although the cell projections in mineralized bone also showed microfilaments. Microfilaments were also found in some dentinoclasts where the actin was located in projections from the ruffled border protruding into the dentine. The localization in the ruffled border corresponded to the distribution of the fluorescent staining of actin. The high amount of actin in osteoblasts and osteocytes may be correlated to the synthesis and transport of the new matrix components. The microfilaments in the clear sealing zone of dentinoclasts were considered to have a function in the sealing of the ruffled b

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1980.tb02477.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY