摘要:

Nudemice of each of the three genetic backgrounds BALB/c, C3H, and C57/BL readily accept skin heterotransplants from inbred Fischer strain rats and other rat strains. Second set transplants from the same donors are also readily accepted. In all instances graft take was uneventful, and the final appearance of grafts was as in normal animals of the donor strain. This event contrasts strongly with that observed in similarnudemice receiving allotransplants. Allograft take was delayed, and although ultimate acceptance was virtually 100 per cent, many grafts evidenced endogenous damage occurring during the acceptance process. This damage was manifested by tufted irregular hair growth in many cases, and by bare atrophic, though vital, areas of graft skin. Also strikingly contrasting were the findings that allografting innudemice excited no cytotoxic antibody response, while cytotoxic antibody titres in serum ofnudemice receiving heterotransplants were moderate to high. Thymus grafting confers capacity for heterograft rejxsection.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03830.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Avian, reptilian and normal human skin grafts are well accepted by thenudemouse, as demonstrated by this and other studies. Histological studies show consistently good incorporation of grafts, and changes in spleen and lymph nodes similar to those observed after other transplants in the animal. The consensus of studies evidence that thenudemouse is the least resistant recipient of grafts of diverse origin among the experimental animals, prepared or otherwise, which are under study today. This is an expression of the extent of the immunological deficiency in thenude.An interesting question asked byCalnein 1970 can be answered. Though its ‘milieu interieur’ could be thought to be widely different from the avian and the reptilian, thenudemouse is an adequate host of avian and reptilian tis

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03831.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

A case of adenoacanthoma of the small bowel is reported. In light of a macroscopic and histological examination, the possibility of a primary tumour is discussed. The conclusion is that the tumour originated in the small intestine.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03832.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Biopsies from primary and secondary malignant melanomas have been studied by light microscopy as well as by fluorescence and electron microscopy. In the two primary tumours, lymphocytes in different stages of transformation were observed at the periphery of the tumour, but evidence of a cytotoxic effect of the immune cells against the tumour cells could not be obtained. Plasma cells were few and deposits of γ‐globulins were not observed outside these cells. In 13 of the 15 metastatic tumours, lymphocytes and plasma cells were observed among the tumour cells, the latter being the most prevalent cell type. In 11 patients, γ‐globulins were found on the surface of the tumour cells and in 7, a variable proportion of the cells showed specific cytoplasmic fluorescence. In one tumour, ultrastructural changes, interpreted as changes due to immune cytolysis, were observed. Viruslike particles were not observed in any

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03833.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Two oxygen‐containing derivatives of unsaturated fatty acids, methyl 12‐oxo‐trans‐10 octadecenoate and methyl hydroxyoctadecadienoate, both highly active in the newt test for carcinogenicity, have been tested as complete carcinogens and as promoting agents by application to the skin of ST/a mice. Through observation for two years the tumourigenic effect was recorded and compared with that of methyl oleate and of adequate positive control substances: 7,12‐dimethylbenz[α]anthracene and croton oil. Both methyl oxooctadecenoate and hydroxyoctadecadienoate showed a significant potency to promote the induction of skin papillomas. The genesis of malignant skin tumours was promoted by methyl oleate and oxooctadecenoate, and all the three methyl esters had a weak activity as complete carcinogens to the skin. As a systemic effect all the esters promoted the induction of malignant lymphomas, most markedly methyl oxooctadecenoate. Even without preceding initiation this ester and methyl oleate had some activity in lymphoma cancerogenesis. The various observations show that, when tested by our experimental procedures, methyl oxooctadecenoate is the ester most active in the induction of tumours, and the effect is essentially that of a promoter. The relation between molecular configuration and carcinogenicity of fatty acids is

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03834.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

The aim of this investigation has been to examine which histological parameters are of value in the dating of the corpus luteum of menstruation. Further to express these parameters in a graphical form which is of practical use for a histological dating. The material comprises histological specimens of the ovaries from 24 women selected according to strongly limiting criteria. The following histological parameters have been found useful for the dating: 1) Extravasated blood and capillaries in the granulosa cell layer. 2) The size of the granulosa cells. 3) Degeneration of the granulosa cells. 4) Invasion of theca cells into the granulosa cell layer. 5) Organization of the central cavity. These parameters have been graded semiquantitatively, and the results have been the basis of the curves prepared by electronic data processing of the observations. Finally some indications for histological dating of the corpus luteum of menstruation are mentioned.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03835.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

The following topics are presented: 1) an enzymatic method for selective separation of hyperplastic epidermis from hair‐bearing mice and its dissociation into free cell preparations rich in metaphase cells, 2) a quantitative cytochemical test (cytofluorometric determination of Feulgen‐DNA) on the retainment of Feulgen reactivity by the dissociated cells, and 3) an example of the application of the method to investigating chemical skin carcinogenesis. It is possible by this method to assay cytophotometrically the DNA of epidermal cells at the same phase of the generative cycle. It turned out that the DNA content of epidermal metaphase cells in the group of mice treated with a carcinogen and mechanical irritation (3 weeks) was significantly greater than the corresponding value of the group which received only acetone and mechanical irritation. This seems to suggest that a treatment with a carcinogen causes fairly early changes in the nuclear material of the germinative tissue. The method described offers new possibilities for quantitative cytochemical characterization of the nuclear changes associated with experimental carcinogenesis, because it eliminates the sources of error normally present in section preparations and the problems of interpretation caused by the cell cycle, and permits the use of cytofluorometry, a method considerably more sensitive than absorption cytophotome

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03836.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

The alterations preceding invasive carcinoma and the development of frank carcinoma have been investigated in 116 mice following intravaginal painting with 3,4‐benzpyrene. The subsequent epithelial changes were graded in increased order of deviation from the normal; atypia grade I, II, III, questionable invasive and frankly invasive carcinoma. Slight atypia (grade I) was observed following 2 months application of the carcinogen. Atypia grade II occurred after 3 and 4 months' treatment. After 5 months, atypia grade II or III, questionable or invasive carcinoma developed. Ninety‐two per cent of the mice with questionable or invasive carcinoma had in addition intra‐epithelial atypias with buds. Invasion was seen to originate at the tip of epithelial buds with severe cellular atypias. The findings strongly suggest an association between epithelial buds with cellular atypias and progression to invasive carc

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03837.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Cervico‐vaginal smears from 256 patients with carcinoma in situ and 103 with microinvasive carcinoma were investigated. The malignant cell population was classified according to the predominant cell type into 4 groups; undifferentiated, basal, parabasal, and mature. Carcinoma in situ could not be differentiated from micro‐invasive carcinoma on the basis of the cytologic appearance in smear preparations, not even by detailed cell analysis including micrometric determinati

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1974.tb03838.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY