年代:1980 |
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Volume 88A issue 1‐6
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51. |
THE EFFECT OF FIXATION AND TRYPSINIZATION ON THE IMMUNOHISTOCHEMICAL DEMONSTRATION OF INTRACELLULAR IMMUNOGLOBULIN IN PARAFFIN EMBEDDED MATERIAL |
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Acta Pathologica Microbiologica Scandinavica Section A Pathology,
Volume 88A,
Issue 1‐6,
1980,
Page 369-376
MARIANNE JACOBSEN,
PER P. CLAUSEN,
SUSANNE SMIDTH,
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摘要:
Using an indirect labelled immunoperoxidase technique the influence of fixation time on the antigenicity of intracellular immunoglobulin in lymphoid tissue fixed in buffered formalin has been investigated. Within a fixation period of 96 hours a decrease of 15% of stainable immunoglobulin containing cells was found, for every 24 hours the fixation time was prolonged. By comparing sections from tissue fixed in buffered formalin and selected fixatives (Lillie's AAF, Bouin's fluid, Clarke's fluid and 96% ethanol 1% acetic acid (E‐A) processed at 4°C and at 25°C) an increased number of stained immunoglobulin containing cells was found in tissue fixed in Lillie's AAF, Bouin's fluid, Clarke's fluid and E‐A processed at 4°C. No difference was found between tissue fixed in buffered formalin and E‐A processed at 25°C. In addition the effect of pretreatment of the sections with trypsin on the number of stainable immunoglobulin containing cells was investigated. Trypsinization of sections from formalin fixed material increased the number of stainable cells substantially. No essential effect was seen on tissue fixed in Lillie's AAF and Bouin's fluid. In contrast trypsin treatment of sections from tissue fixed in Clarke's fluid and E‐A completely destroyed the tissue. No differences were observed between different immunoglobulin classes examined as regards the effect of fixation time, fixatives and try
ISSN:0365-4184
DOI:10.1111/j.1699-0463.1980.tb02508.x
出版商:Blackwell Publishing Ltd
年代:1980
数据来源: WILEY
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52. |
ENZYMECYTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY IN MONOCLONAL GAMMOPATHY AND REACTIVE PLASMACYTOSIS |
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Acta Pathologica Microbiologica Scandinavica Section A Pathology,
Volume 88A,
Issue 1‐6,
1980,
Page 377-382
PREBEN JOHANSEN,
MOGENS KROGH JENSEN,
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摘要:
Peripheral blood smears and bone‐marrow smears from 29 patients with malignant M‐components (25 with multiple myeloma and 4 with malignant lymphoma), 13 patients with benign monoclonal gammopathy (BMG), and 20 patients with polyclonal reactive plasmacytosis were examined by leucocyte alkaline phosphatase score (LAP‐score) and by acid phosphatase score in plasma cells from bone‐marrow smears. Furthermore, tissue sections from marrow biopsies from all patients were examined by the three‐layer unlabelled immunoperoxidase technique to detect cytoplasmic immunoglobulin. The LAP‐score was significantly higher in patients with malignant M‐components than in patients with BMG and also higher in IgA and IgG myeloma than in IgA and IgG BMG, but the latter difference was not significant. Furthermore, a significant positive correlation between paraprotein concentration and LAP‐score was found in multiple myeloma. Acid phosphatase score in plasma cells showed no clear distinction between multiple myeloma and BMG. Immunohistochemical examination showed a distinct monoclonal pattern in both multiple myeloma and BMG, allowing identification of the M‐cxsomponent which in all cases corresponded to the M‐component detected by serum examination. Cells producing immunoglobulin classes not matching the M‐component were more rare in multiple myeloma than in BMG, but the difference between the two conditions was quantitative and allowed
ISSN:0365-4184
DOI:10.1111/j.1699-0463.1980.tb02509.x
出版商:Blackwell Publishing Ltd
年代:1980
数据来源: WILEY
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53. |
THE ANTRAL GASTRIN‐PRODUCING CELLS IN DUODENAL ULCER PATIENTS |
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Acta Pathologica Microbiologica Scandinavica Section A Pathology,
Volume 88A,
Issue 1‐6,
1980,
Page 383-386
H. OVERGAARD NIELSEN,
K. BIRGER JENSEN,
L. A. CHRISTIANSEN,
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摘要:
A density study of the antral gastrin‐producing cells has been performed, before and after eight weeks of treatment with a histamine H2‐receptor antagonist (cimetidine 1 g per day), in a series of 38 patients suffering from chronic duodenal ulcer. The treatment produced a significant increase in numerical density and a decrease in mean cell volume. The volume density of the cells was unchanged, suggesting that the elevated serum‐gastrin during treatment may be the result of gastrin‐cell hyperplasia, without change in the total gastrin‐cell volume. The authors suggest that G‐cell hyperplasia demonstrated during cimetidine treatment may be a factor of importance with regard to the rapid recurrence of many ulcers after discontinuation of th
ISSN:0365-4184
DOI:10.1111/j.1699-0463.1980.tb02510.x
出版商:Blackwell Publishing Ltd
年代:1980
数据来源: WILEY
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54. |
IN SITUIDENTIFICATION OF INFLAMMATORY CELLS IN MALIGNANT, NON‐LYMPHOID HUMAN TUMOURS |
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Acta Pathologica Microbiologica Scandinavica Section A Pathology,
Volume 88A,
Issue 1‐6,
1980,
Page 387-395
J.‐L. SVENNEVIG,
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摘要:
Inflammatory cells (the term is considered to include lymphocytes, plasma cells, macrophages, mast cells and PMN) were identified and quantitated in sections from human carcinomas. Two types of cellular infiltrates are described. In the stroma surrounding the cancer tissue (peritumoural) and, to a lesser degree, also within the malignant tissue (intratumoural), lymphocytes, plasma cells, macrophages and mast cells dominated, whilst numerous polymorph nucleated cells (PMN) and aggregates of macrophages characterized central tumour necroses. The demonstration of both T‐lymphocytes and macrophages in and around the cancer tissue supports the view that a local immune reaction is initiated by the tumour, and the consistent finding of IgG, IgA and IgM plasma cells at the borders of the carcinomas, indicates that plasma cells are also part of an immune response at the tumour site. This view is further supported by the demonstration of an increased proportion of IgG containing plasma cells in the stroma of colon tumours, compared with normal mucos
ISSN:0365-4184
DOI:10.1111/j.1699-0463.1980.tb02511.x
出版商:Blackwell Publishing Ltd
年代:1980
数据来源: WILEY
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55. |
ULTRASTRUCTURE OF THE TAIL STUMP SPERM DEFECT IN THE BULL |
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Acta Pathologica Microbiologica Scandinavica Section A Pathology,
Volume 88A,
Issue 1‐6,
1980,
Page 397-405
ERIK BLOM,
AKSEL BIRCH‐ANDERSEN,
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摘要:
A characteristic major sperm defect, with about 60% of the sperm cells showing a rudimentary tail stump, was found in two sterile Danish bulls of imported origin. The condition seldom occurs, but seems to be widespread and has been observed in several breeds of cattle and in different animal species, including man. Electron microscopy of ejaculated sperm and testicular tissue from one of the bulls has shown that a dysfunction of the centriolar apparatus during spermiogenesis is likely to be the cause of the defect. In ejaculated defective sperm the proximal centriole is often found connected with the normally transitory organelle, the centriolar adjunct. In the defective spermids the distal centriole has lost the faculty to form axonemal fibers, so that no tail formation can take place.
ISSN:0365-4184
DOI:10.1111/j.1699-0463.1980.tb02512.x
出版商:Blackwell Publishing Ltd
年代:1980
数据来源: WILEY
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56. |
LATE EFFECTS OF COLCHICINE ON THE BURSA OF FABRICIUS AFTER NEONATAL APPLICATION ON THE ANAL LIPS OF CHICKENS |
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Acta Pathologica Microbiologica Scandinavica Section A Pathology,
Volume 88A,
Issue 1‐6,
1980,
Page 407-413
TIMO ROMPPANEN,
TAPANI E. SORVARI,
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摘要:
One week after the application of colchicine on the anal lips of chickens, the bursa of Fabricius was devoid of lymphoid cells and contained only a few epithelial buds, all of which were later repopulated with lymphoid cells to form mature lymphoid follicles. After the intraperitoneal injection of cyclophosphamide, numerous epithelial buds were found in the bursa, and at the age of six weeks only a few of them were populated with lymphoid cells. On week 6, the bursal weights of the chickens treated with colchicine were only about 10% of the normal, and about 80% of the bursal weights of the cyclophosphamide‐treated chickens. The colchicine treatment affected the morphology of the spleen less than the treatment with cyclophosphamide. It is concluded that this new model of chemical bursectomy differs distinctly from the cyclophosphamide model and therefore serves as a new tool in studying bursal function in chicke
ISSN:0365-4184
DOI:10.1111/j.1699-0463.1980.tb02513.x
出版商:Blackwell Publishing Ltd
年代:1980
数据来源: WILEY
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57. |
HISTOLOGIC TYPING OF BREAST CANCER |
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Acta Pathologica Microbiologica Scandinavica Section A Pathology,
Volume 88A,
Issue 1‐6,
1980,
Page 415-416
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ISSN:0365-4184
DOI:10.1111/j.1699-0463.1980.tb02514.x
出版商:Blackwell Publishing Ltd
年代:1980
数据来源: WILEY
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58. |
ESTIMATION OF THE VOLUME OF HUMAN ANTRAL MUCOSA BY MEASURING THE ANTRAL LENGTH ALONG THE LESSER AND GREATER CURVATURE |
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Acta Pathologica Microbiologica Scandinavica Section A Pathology,
Volume 88A,
Issue 1‐6,
1980,
Page 417-419
H. Overgaard Nielsen,
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摘要:
A significant positive correlation was found in 11 antrectomy preparations between the product of antral length along the lesser and greater curvatures and the antral mucosal volume (coefficient of correlation 0.9297). This means that the antral mucosal volume can be estimated by measuring the antral length along the curvatures, a procedure possible during operation with the organ in situ.
ISSN:0365-4184
DOI:10.1111/j.1699-0463.1980.tb02515.x
出版商:Blackwell Publishing Ltd
年代:1980
数据来源: WILEY
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