摘要:

In order to select a sensitive method for demonstrating blood group substances A and B in sections of oral epithelium, biopsies and exfoliated cells from the oral epithelium were investigated with both a double‐layer immunofluorescence (IF) staining method and the mixed cell agglutination (MCA) reaction. Eighteen anti‐A and 16 anti‐B sera were tested. Fourteen anti‐A sera were able to react in the IF method and only 9 in the MCA reaction. All tested anti‐B sera produced positive IF staining; eleven also reacted in the MCA reaction. Blood group antisera could in most cases be used in higher titres in the IF method than in the MCA technique. In the IF method, porcine antihuman IgG/FITC and porcine antihuman IgM/FITC were used as the second layers. Blood group antisera could generally, but not invariably, be used in higher titres with antihuman IgM/FITC. The importance of careful matching of blood group antiserum and conjugate is e

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00302.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

NMD provoked by diets containing profuse quantities of unsaturated fatty acids occurred in three calves, while four other calves were protected with vitamin E. The diseased calves presented macroscopic and light‐microscopic waxy degenerative changes typical of NMD. Electron microscopy of degenerated areas in the myocardium and in skeletal muscle, revealed that the presence of strongly electron‐dense formations between intact myofibrils represented the first stage in the disease. The localization, size and shape of these formations were consistent with features in the mitochondria of intact cells. In the same areas, intact mitochondria were mostly absent. In some areas, mitochondria with only some electron‐dense crystals could be seen. In further advanced stages of the disease, similar electron‐dense formations might be seen, but they were surrounded by a structureless, necrotic mass without myogenic striation. Abundant, immature cells, most of which were difficult to identify were seen between the destroyed muscle cells. The cells which had the nature of satellite cells and were located below the intact external lamina of the degenerated cell were intrepreted as my

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00303.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Electronmicroscopy has been performed on biopsy material from human striated muscle—obtained on the basis of voluntariness and good‐will of eleven young, healthy students. The results are presented in this paper, which deals with the following two main subjects: 1. Information concerning the technical procedures used in this laboratory with remarks on certain important points at different stages in the preparation. 2. The results of an extensive electronmicroscopical investigation of these biopsies which revealed a rather pronounced morphological variation in young, healthy, human subjects. The classic electron‐microscopic morphology of striated muscle has in reality for many years been based on animal experiments. This investigation in healthy human subjects has demonstrated a morphology basically identical to that of animal muscle, but it seems as if additional elements must be regarded as necessary parts of a true electronmicroscopic picture of striated muscle i

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00304.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Mouse amyloid induction with casein was accelerated by weekly injections of 108syngeneic non‐sensitized lymph node cells. Injections of spleen cells and of a mixture of thymus and bone marrow cells were without effect. A slight drop in the level of circulating casein‐antibodies was noticed in mice having received lymph node cells. Two explanations of the acceleration were considered. An accelerating factor may be released from desintegrating lymph node cells or lymph node cells are producing the amyloid fibrils which later lodge in the spl

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00305.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The mouse mutant nude suffers a congenital aplasia of the thymus, which results in a nearly total lack of thymus dependent lymphoid cells. In spite hereof amyloidosis was induced in nude mice with prolonged casein stimulation. The development of amyloidosis exhibited the usual pattern of distribution, but the induction time was significantly shorter in nude mice than in normal littermates. No circulating casein‐antibodies were found in nude mice during the development of amyloidosis. The possible role of tolerance as a pathogenetic mechanism in amyloidosis is discusse

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00306.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The splenic release of lymphocytes specifically engaged in an immune response was quantitated in guinea pigs during a secondary response to sheep red blood cells (SRBC). The number of cells forming rosettes with SRBC was compared in splenic venous and arterial blood. The splenic veno‐arterial difference in the number of rosette‐forming cells (RFC) was compared in normal guinea pigs and at different intervals after a booster dose of SRBC, given 3 weeks after a primary dose. In normal guinea pigs there was no release of RFC from the spleen. A small release was found 3 weeks after a primary dose of SRBC. After a booster dose the splenic veno‐arterial difference in the number of RFC increased to a maximal value after 6 days. Simultaneously, the number of RFC was increasing in both the spleen and the blood. The results clearly indicate that the spleen releases lymphocytes with specific antigen receptors during the secondary immune response to

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00307.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

A case of thrombocytopenic purpura, megacaryocytopenia and signs of auto‐immune processes (positive direct Coomb's test) is presented. It was later complicated by an intracranial haemorrhage. At autopsy a thymoma (microscopically a lymphoepithelioma with spindle cell predominance) was found. The possible causal relationship between the two disorders is discusse

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00308.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The relationship of cigarette smoking to coronary and aortic atherosclerosis was analysed in two autopsy series, one comprising 84 men aged 20–77, who had died by violence and another comprising 94 men aged 30–78, who had died suddenly from coronary heart disease. The extent of atherosclerosis was assessed by measuring the surface areas of atherosclerotic lesions by point‐counting technique. In the series of violent deaths, both the coronary and aortic atherosclerosis was more severe in cigarette smokers than in nonsmokers. As could be expected, in the series of men who had died from coronary heart disease and generally had an advanced atherosclerotic involvement of coronary arteries, the severity of coronary lesions showed no relationship to smoking habits. In the series of coronary deaths, however, aortic atherosclerosis tended also to be more extensive in cigarette smokers than in nonsm

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00309.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

In an investigation of liver biopsy specimens from patients with extra‐hepatic biliary obstruction, an increase was found in the products of histochemical reactions for acid phosphatase, beta‐glucuronidase, non‐specific esterase and succinic dehydrogenase around bile pigments, for alkaline phosphatase in sinusoids and canaliculi, for adenosine triphosphatase in sinusoids and some canaliculi with loss in others. Leucine aminopeptidase outlined irregular and thickened canaliculi, gamma‐glutamyl transpeptidase increased number of stained thin can

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00310.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Histological classification of gastric carcinomas into the main types intestinal and diffuse was made according toLaurén'smethod. Autopsy material was compared to surgical material, and metastases were compared to primary tumours, with regard to type distribution and reproducibility. The proportion of diffuse tumours was substantially higher in the total autopsy material than in the total surgical material. This was probably due to a stronger tendency for diffuse tumours to reach an inoperable stage before the diagnosis is made. In patients from whom both surgical and autopsy specimens were available, the same type was diagnosed in both in the majority of cases, and the proportion of diffuse tumours was only slightly higher if typed from the autopsy material than if typed from the surgical material. The reproducibility of histological typing in two independent readings of the same sections was 91 per cent in surgical material and 84 per cent in autopsy material. Metastases reproduced the histological type of the primary tumour in 65 per cent for all metastatic sites combined. Diffuse patterns were most frequently reproduced in metastases to adipose and fibrous tissues, and intestinal patterns were most frequently reproduced in metastases to the liver

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1972.tb00311.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY