摘要:

A new “tidal flow” cultivation system, designed for the cultivation of large amounts of tissue in primary culture is described. In the present cultivation apparatus all explanted tissue shares a common gas phase and a common pool of nutritional medium where variations of pH and glucose concentration are largely kept within physiological limits throughout the duration of the cultivation. Oxygenation of the tissue is facilitated since oxygen diffusion may take place from all sides of the explants and since the tissue is only intermittedly submerged in nutritional medium. Morphologically, the degenerative changes occurring in primary explants of lung tissue are delayed. However, changes of the LDH isoenzyme pattern in favour of M‐LDH activity, which may be considered a sign of dedifferentiation at the molecular level, start immediately and are not prevented by an increased oxygen tension. It is concluded that morphological changes are preceded by changes at the molecular

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02531.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

A cathodic shift of the LDH isoenzyme pattern in oral leukoplakic mucosa, similar to that found in carcinoma of the oral mucosa, has previously been reported. Similar isoenzymatic modifications affecting apparently uninvolved fields of tissue are commonly found in tumour‐bearing organs indicating the presence of macromolecular abnormalities or “field” changes preceding the development of neoplasia. The present study, comparing the isoenzymatic composition of leukoplakic and morphologically unaffected oral mucosa of the same persons, indicates that most leukoplakias may be localized conditions where morphological and molecular changes are confined within the limits of the macroscopic lesion. It is suggested that leukoplakias located within an area exhibiting isoenzymatic “field” changes may be those carrying a true preneoplastic

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02532.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The lactate dehydrogenase isoenzyme pattern of biopsies from Indian oral leukoplakia, submucous fibrosis and carcinoma of the oral mucosa as well as that of oral mucous membrane biopsies from clinically normal Indian persons has been studied. The LDHIV/LDHIIisoenzyme ratios found for leukoplakias, carcinomas and control biopsies did not differ significantly. The mean isoenzyme ratio of Indian control biopsies was significantly above that reported for Danish control biopsies. Submucous fibrosis was accompanied by a significant depression of the isoenzyme ratio. No clear correlation between LDH isoenzyme pattern and epithelial thickness was found. Smoking and chewing habits, which may be correlated with prevalence and localization of oral leukoplakias and carcinomas, did not significantly influence the isoenzymatic structure. Although final conclusions cannot be drawn, the high isoenzyme ratio of the Indian control material may represent an early precancerous condition, unrelated to smoking and chewing, preceding the histopathological manifestations of precancerous lesions.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02533.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

A changed growth pattern is reported in subcutaneous transplants of Ehrlich's carcinoma in mice following systemic treatment with Butazolidine. An acute inflammatory response was seen at the edge of the tumour transplant in the untreated control mice. Treatment with Butazolidine favoured the development of denser connective tissue which ran along the edge of the transplant rather than mingling with it. The change in the orientation of the fibroblasts and capillaries was accompanied by an increase in lymphocyte and macrophage infiltration and tumour cell necrosis. It is suggested that the tumour's ability to induce an acute inflammatory response may be a prerequisite for stroma formation and vital to the establishment of infiltrative growth.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02534.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Changes in the lymph nodes, spleen and thymus are described following a single intravenous dose of PHA‐P in mice. On the basis of histological studies it is suggested that the primary picture of regular proliferation may be due to stimulation of the lymphoid cellsin situ, while the later grossly irregular proliferation found in the lymph nodes in particular may be due to secondary stimulation of this altered population by PHA stimulated cells from the thymus. In view of these findings caution in the clinical use of this drug is urge

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02535.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The development of a malignant tumour in a stillborn infant is reported. The tumour was localized to the maxilla and metastases to lymph nodes, liver, and adrenal glands were in evidence. By microscopy of the primary tumour the latter was found to be characterized by a sporadic occurrence of pigment; in addition, metastases to the adrenal glands were found to comprise fontana‐positive cells. If these features be related to the localization of the tumour and the age of the patient it seems natural to suggest that it is a matter of a melanotic progonoma although this type of tumour previously has been considered a benign growt

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02536.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Previous studies have shown that human lymphocytes culturedin vitroshow metabolic adaptations to variations in oxygen tension. In order to establish whether morphological adaptations take place under similar conditions, lymphocytes were cultured with phytohaemagglutinin at 5, 20 and 50 per cent 02. After 48 hours, the mitochondria were stained with Janus Green B and counted. The average number per cell was found to be independent of pO2but varied with cell area. Cellular ultrastructure was studied in sections of fixed and stained preparations, and isolated mitochondria were subjected to hypotonic treatment and negative staining in order to investigate the stalked particles on the inner membrane. None of these methods disclosed any differences between cells cultured at different oxygen tensions.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02537.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Fanconi's aplastic anaemia is reported in a 7‐year‐old child, in whom the diagnosis was verified by chromosome analysis on peripheral blood. The characteristic chromosome aberrations and their specificity are reviewed. The parents and three sibs of the patient presented normal chromosome patterns. It is mentioned that an increased incidence of leukaemia is demonstrated in patients with Fanconi anaemia, and that, in a number of other disorders with chromosome aberrations, an increased tendency to development of malignancy is s

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02538.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

It is a characteristic feature of human cancerous effusions that the cells show great variation and, in particular, it is generally emphasized that therapy does not exert any influence on the chromosomal constitution of the cancer cells. Selection of a cell line during treatment is stated to occur very infrequently. Such a selection of a cell line with a constant number of chromosomes was demonstrated by repeated chromosome analyses in a patient during chemotherapy. The results of the studies are described, and the practical and theoretical aspects are commented upon. – Alterations in the karyotypic profiles in cancerous effusions during treatment with chemotherapeutic agents will require consideration of changing to another agen

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02539.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The cellular decay of thymus single cell suspensions was studied by combined nigrosin dye exclusion and3H‐thymidine autoradiography. Nigrosin stained, Feulgen dyed thymic cell smears were examined at intervals after injection of3H‐thymidine into NMRI mice aged 30–45 days. Lymphoid cells of DNA‐synthesizing as well as non‐DNA‐synthesizing populations were found to decay. The decaying cells descendig from DNA‐synthesizing cell populations took up nigrosin mainly in the premitotic phase of their generation cycle. Percentage of non‐viable DNA‐synthesizing cells (8 per cent) and the average nigrosin staining time of the DNA‐synthesizing cell population (2 hours) indicated a decay rate in this cell fraction of 4 per cent per hour. The proliferation rate of the DNA‐synthesizing cell population was found to be 10 per cent per hour. Calculations including these values gave an average DNA‐synthesizing cell cycle time of 7 hours. Throughout the experimental period of 14 days, the non‐DNA‐synthesizing cell population showed random cellular decay. However, a specific cellular decay took place 3–5 days after injection of3H‐thymidine, indicating a life span of approximately 4 days for many small thymocytes. The total thymic grain counts were used as a measure of organ radioactivity. The first days after injection showed massive reutilization of3H‐thymidine released by cell decay during this period. In conclusion, a combination of autoradiography and dye exclusion of unfixed thymic cell suspensions leads to useful kinetic parameters of

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1970.tb02540.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY