摘要:

The effect of different DEAE‐dextran derivatives on the surface charge of tumour cells has been investigated. The DEAE‐dextran varied in molecular weight and charge density (degree of substitution with diethyl‐amino‐ethyl groups). It was shown that the binding to the tumour cell surface and the ability to neutralize the negative charge of the cell surface was dependent upon the molecular weight and the charge density. Higher molecular weight and higher degree of substitution gave a stronger binding. The toxicity of the various compounds in mice and amoebae was correlated to the cell surface affinity. The biological effect of the different drugs was investigated in the C3H mice using the transplantable ascites tumour JBI which is a plasmocytoma. The DEAE dextran has an inhibitory effect on the growth of transplanted tumours. This effect was greatest for the compound with the highest molecular weight (2 mill.) and the highest degree of substitution (50 per cent), and was negligible for a compound with molecular weight of 64, 000 and 13 per cent subst

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03316.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Vital and postmortal frostbites of various ages in the skin and adipose tissue of guinea pigs were examined by histochemical methods. The microscopic lesions were slight, both in the skin and in the adipose tissue. Nuclear changes were seen in vital as well as postmortal frostbites. Four hours after the freezing, NAD‐diaphorase and lactate dehydrogenase showed decreasing reactions in the epithelial cells and fibroblasts, and later in fat cells of the frozen area, and the reaction had almost entirely disappeared after 24 hours. In two to four hours after freezing, diffuse extracellular staining of naphthyl amidase and esterase appeared in the frozen tissue. Small fat cell necroses were noticed after a reaction time of four hours. Exudation was observed in the sections within one to two hours after vital freezing. No morphological signs of damage to vascular walls were seen. In blood vessels, closely packed red cells filled the lumen, but real thromboses with fibrin and platelets were rare. The inflammatory reaction started in the skin within one hour and in the adipose tissue, within 30 minutes. Mononuclear macrophages appeared within four hours in the skin and within two hours in the adipose tissue. In frostbites of 16 hours' age epidermal blisters filled with fibrinous exudate and inflammatory cells were seen. The blisters were surrounded by a semicircular demarcation zone of polymorphonuclears and macrophages developing within 8–16 hours. In the adipose tissue new fibroblasts appeared within four hours after freezing. In the skin, epithelial proliferation was seen after 36 hours around the blisters. The fastest and most reliable vital sign was the post‐freezing inflamm

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03317.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Serum drawn on the eight and ninth day after the combined administration of busul‐phan and chlorambucil caused a distinct increase in the incorporation of3H‐thymidine compared with control serum. This was taken as evidence that there is in the serum at these points of time a factor that stimulates the proliferation of bone marrow cells and is similar to antichalone in its properties. This view was substantiated by the finding that serum taken on the ninth day also increased the uptake of3H‐adenine. Sera taken on the second and sixth day did not influence the incorporation of3H‐thymidine compared with the control serum. The stimulating factor was at first weaker after the combined administration of the two cytostatics than after chlorambucil alone, but the situation was reversed after the ninth day. This was in good accord with the changes in the blood picture. The sera now had a distinctly greater stimulating effect on the incorporation of3H‐thymidine than the sera taken after busulphan treatment. The serum taken after two days was an exception. The modes of action of busulphan and its relationship to chalone are

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03318.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Enteric infection withYersinia enterocoliticais often associated with small focal non‐specific ulcerations in the appendix and sometimes with basophilic stem cell hyperplasia or proliferation of large pyroninophilic cells in mesenteric lymph nodes. This proliferation correlates comparatively well with an early antibody response. The clinicopathological and immunological aspects of the findings are discusse

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03319.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

One to eight weeks after autotransplantation of isolated endometrial or myometrial tissue into the eyes of rabbits the transplants were found to contain higher renin concentration than the tissuein situ. Castration‐resulted in a marked decrease, and subsequent estradiol treatment in a significant increase in the renin concentration of the grafts, as well as of the tissuein situ. Renin is thus formed in both endometrial and myometrial tissue of the rabbit uterus. Grafts of ureter and of renal artery contain the same, extremely low, concentrations of renin as these tissuesin situ. The iris from eyes grafted with endometrium contained renin, while this was not the case in eyes with myometrial graft

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03320.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

In different strains of mice the sum of renal and submaxillary renin was found to vary from about 10 to about 1000 Haas‐Goldblatt units, about 7 to well over 98 per cent of which was found in the submaxillary glands. The plasma renin and angiotensinogen concentrations were independent of these variations. Followingsubmaxillary sialo‐adenectomythere was only a slight decrease, if any, in plasma renin or increase in plasma angiotensinogen. Contrary to this,nephrectomywas followed by a marked decrease in plasma renin and a marked increase in plasma angiotensinogen, both of which were independent of the highly varying amount of submaxillary renin left in the organism. The postnephrec‐tomy increase in angiotensinogen was further independent of variations in the pre‐operative plasma and renal renin, supporting the previous conclusion that it must be due to loss of some internal factor other than renin or to loss of some external renal f

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03321.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Ovarian tumours in mice can be induced by 9:10‐dimethyl‐l: 2‐benzanthracene (DMBA). DMBA's primary action in the mouse ovary is the destruction of small oocytes. Whether the parent DMBA or some break‐down product is the effective carcinogen and whether prolonged retention of the carcinogenic principle in the target organ is a prerequisite for a neoplasma to develop are unknown. These questions were studied in experiments with tritiated DMBA, comparing the primary cytological effect of DMBA with the presence of radioactivity in tissue extracts. Radioactive compounds extracted from tissues after treatment with DMBA‐3H were analysed by two‐dimensional chromatography. The results suggest that DMBA itself is the effective initiator in ovary carcinogenesis in mice rather than somein vivoconversion of the chemical. The different rates at which small oocytes are destroyed after oral and intraperitoneal administration of the carcinogen can be directly correlated with different levels of carcinogen obtained in the tissues after the two routes of administration and to their different excretion rates. It is concluded, that the carcinogenic effect of DMBA on mouse ovaries is related to the immediate destruction of small oocytes, whereas the prolonged retention of DMBA after intraperitoneal injection is of no significance for the neoplastic

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03322.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

The material comprises 67 percutaneous liver biopsies all with fatty change as the chief morphological diagnosis. Among others biopsies with cirrhosis and Mallory bodies have been excluded. Lipogranulomas have been found in 43 out of 67 biopsies. A description of lipogranulomas is given, and three types are described according to size and age. Steatosis is a conditio sine qua non for the development of lipogranulomas. Biopsies with lipogranulomas show higher histologic activity (lytic liver cell necrosis, acidophilic bodies and rnesenchymal reaction) and more parenchymal fibrosis than biopsies without. The patients with lipogranulomas have higher S. G. O. T. values. Whereas lipogranulomas type 1 and 2 can disappear without sequelae there is good reason to believe that lipogranulomas type 3 may give rise to development of connective tissue in the parenchyma.

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03323.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Successful heterotransplantation of 6 out of 9 consecutively transplanted adenocarcinomas of the human colon and rectum to the mouse mutant Nude is reported. Serial growth for 2–8 transfers so far was obtained in 5 cases. The transferred tumours grow locally, and metastatic growth has not been observed with certainty. The histological and cytological appearance of the transferred tumours is in full accordance with that of the primary tumours. The mutant Nude is suggested as a host for human malignant tumours as a basis for the study of tumour growth and for therapeutic assay

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03324.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

The histological changes in 57 renal allografts from rabbits presensitized by multiple skin grafts have been studied semiquantitatively. No histological changes, except oedema, were observed in 11 allografts removed within 6 hours after transplantation because of clinical rejection. Eleven allografts removed 1–5 days after transplantation showed the pattern of hyperacute rejection: extensive glomerular microthrombosis and cortical necrosis. Circulating cytotoxic antibodies against donor leucocytes did not always induce hyperacute rejection, but they were found in all the cases of hyperacute rejection tested for their presence. The most conspicous difference between the allograft reaction in sensitized and in non‐sensitized recipients was the early appearance and the increased frequency of exudative glomerulitis and glomerular microthrombosis. In addition proliferation of the endothelial cells of the glomeruli and vessels and vasculitis were more frequent in sensitized rabbits. The results indicate that presensitization accelerates allograft rejection in rabbits and suggest that this process, which in several cases was morphologically similar to human hyperacute allograft reaction, is to some extent related to circulating antibod

ISSN:0365-4184    

DOI:10.1111/j.1699-0463.1971.tb03325.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY