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| 51. |
International programme on the serological detection of bacterial, fungal and viral pathogens of protein pea seeds1 |
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EPPO Bulletin,
Volume 25,
Issue 1‐2,
1995,
Page 393-401
N. F. LYONS,
J. D. TAYLOR,
S. J. ROBERTS,
Y. MAURY,
C. DUBY,
K. MASMOUDI,
S. FARIS‐MOKAIESH,
R. CORBIÈRE,
E. MENDES‐PEREIRA,
D. SPIRE,
R. SAMSON,
L. MALANDRIN,
C. GRONDEAU,
A. A. J. M. FRANKEN,
B. A. FRAAIJE,
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PDF (543KB)
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摘要:
Arable (protein) peas are widely grown in northern Europe, specifically for use in animal feed. The main production areas (1 million ha) are France, UK and Denmark; The Netherlands is a major seed producer. There are a number of important seed‐borne pathogens, including bacterial blight (Pseudomonas syringaepv.pisi), leaf and pod spot (Mycosphaerella pinodes) and pea seed‐borne mosaic potyvirus (PSbMV), that cause crop losses. There is a need for improved rapid methods for the detection of these pathogens both to monitor the production of high‐quality seed and for quarantine purposes. Using knowledge of the specificity of antibodies raised to these organisms, techniques were developed for simultaneous detection of the pathogens from the same seed sample. Methods were developed that permitted the detection of 101–102bacterial cfu ml‐1in seed‐soak fluid, soluble antigen from seed with and without obvious fungal infection and the presence of PSbMV within the embryo of homog
ISSN:0250-8052
DOI:10.1111/j.1365-2338.1995.tb01482.x
出版商:Blackwell Publishing Ltd
年代:1995
数据来源: WILEY
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| 52. |
Schemes and methods for production and certification of virus‐free grapevine planting material1 |
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EPPO Bulletin,
Volume 25,
Issue 1‐2,
1995,
Page 403-409
I. V. POP,
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PDF (373KB)
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摘要:
Based on experience in Romania and data from the literature, a scheme for the production of certified grapevine propagating material is presented. In addition, details of the test methods for determining presence of grapevine viruses are described.
ISSN:0250-8052
DOI:10.1111/j.1365-2338.1995.tb01483.x
出版商:Blackwell Publishing Ltd
年代:1995
数据来源: WILEY
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| 53. |
Detection methods for pests in plant germplasm introduced into Brazil for research purposes1 |
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EPPO Bulletin,
Volume 25,
Issue 1‐2,
1995,
Page 411-417
M. F. BATISTA,
V. L. A. MARINHO,
M. E. N. FONSECA,
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PDF (386KB)
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摘要:
The National Center for Genetic Resources and Biotechnology (CENARGEN/EMBRAPA) is responsible for the introduction, exchange and post‐entry quarantine of plant germplasm in Brazil. CENARGEN handles about 12000 accessions per year including imported, exported and domestic transit material. The quarantine service is composed of six laboratories dealing with insects, fungi, bacteria, nematodes, virus and viroids, and tissue culture, respectively. There are also facilities for fumigation and for chemical and heat treatments. The detection methods used in the inspections are described, and results on the pests detected are presente
ISSN:0250-8052
DOI:10.1111/j.1365-2338.1995.tb01484.x
出版商:Blackwell Publishing Ltd
年代:1995
数据来源: WILEY
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Volume 25 issue 1‐2