摘要:

SYNOPSISThe exocrine pancreatic cell of the mouse was the test object for the investigation. Tissue fixed with a 0.5 p.c. aqueous solution of chromium trioxide was embedded in Araldite and sections were examined with the electron microscope.Chromium trioxide does not convert the cytoplasm into a coagulum. What appears to be a coagulum in optical microscopy is in fact a three‐dimensional network of ergastoplasm (endoplasmic reticulum), the cisterna of which retain their identity (though the ribosomes are not preserved).The mitochondria are not destroyed, but no internal structure is seen. No vestige of the Golgi element remains.The inner nuclear membrane is preserved, the outer only here and there. Nothing that could be interpreted as a thread‐like structure is seen in the nucleus. The nucleolus appears homogene

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04523.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY

摘要:

SYNOPSISThe preservation and identification of free lipids is most difficult because they are very readily dissolved by the usual embedding media. At present only unsaturated triglycerides fixed by postchromation and lipofuscins can survive this extraction. There is no general fixative for lipids. A few unusual embedding media will preserve small droplets of lipids, but they are technically far from satisfactory. Included in this group is a new medium—urea‐formaldehyde. Some general stains for lipids have been developed; the results are, again, usable but rather unsatisfact

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04524.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY

摘要:

SYNOPSISThe 1–2 μ protein fibres forming an internal lining to the fragile arenaceous shell ofHaliphysemaare made of 0.2 μ diameter, cross‐striated fibres, which have an axial period of approximately 670 A and six distinct intraperiod bands. It is suggested that the fibres may be collagen, a fibrous protein generally considered to occur in all animal phyla except the Protozoa. The flexibility of the shell imparted by such a collagenous sheath could account for the survival of this bizarre protozoan in the agitated conditions of the inter‐tid

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04525.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY

摘要:

SYNOPSISThis note describes a new technique for ensuring firm adhesion of micro‐organisms to slide

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04526.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY

摘要:

SYNOPSISA method for re‐mounting 4 μ thick epoxy resin sections of botanical material after optical inspection is described. The sections are cemented with resin monomer to the tips of pre‐cast resin blocks in a specially made apparatus which can deal with eight sections in one opera

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04527.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY

摘要:

SYNOPSISA technique is described for the storage of biological materials in air tight sachets made from a new aluminium‐polythene laminate. Tissues containing highly mobile soluble radioactive material have been stored for periods up to six months in these sachets which are stored in a liquid nitrogen refrigerator. This method of storage preserves the tissues in such a way as to prevent ice crystal damage, and also prevents cross‐contamination of radioactive material which would result from contact between radioactive specim

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04528.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04529.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY

摘要:

SYNOPSISA simple method of combined dye perfusion and aldehyde fixation for electron microscopy is described. The technique has been found useful in the selection of accurately localized samples of perivascular tissue.

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04530.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY

摘要:

Book reviewed in this article:Advances in Optical and Electron Microscopy. Edited by R. Barerand V. E. CosslettMicroscopy for Chemists. By H. F. SchaefferIntroduction to Microscopy. By G. W. WhiteMedical Colour Photomicrography. By D. SzabóThe Chromosomes of the Algae. Edited by MaudB. E. G>odwardCells and Cell Structure. By E. H. Merce

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1967.tb04531.x

出版商:Blackwell Publishing Ltd    

年代:1967

数据来源: WILEY