ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02089.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02090.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02091.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02092.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

Thecortex of the thymus of leukemic chickens diseased with the BAI strain A tumour virus responds to the infection by a strong adenosinetriphosphatase activity and by a progressive replacement of the normal lymphoid cells by a new population of blast‐like elements (de Thé,et al.). Electron microscopic studies of this ATPase activity were made on tissue fixed overnight in formol calcium (Baker, 1946) or formol phosphate (Lillie, 1954). Frozen 40 μ sections (Holt&Hicks, 1961) were incubated 4 min at room temperature in the Wachstein and Meisel medium (Wachstein&Meisel, 1957) and processed for electron microscopy.Positive reaction was found at the cytoplasmic membrane of the blast‐like cells, and no reaction occurred in association with lymphoid cells of the morphology of those of the normal cortex. Lead phosphate precipitate also occurred about virus particles (de Thé,et al., 1962; Novikoff,et al.) in the intercellular spaces indicating the usual ATPase activity of the agent (Beard, 1962) in this situation.Studies were made in the same way on the nephroblastoma (Heine,et al., 1962) arising in birds inoculated with BAI strain A virus and not dying of leukemia. After incubation for 10 to 50 min at 37°C in the ATPase medium, the different components of the tumour, namely, nephronic structures, sarcomatous elements and cartilage as well as the virus formed in these different components gave varied

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02093.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02094.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02095.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

Manyinvestigations on the structure of cell membranes have been carried out on OsO4or KMnO4fixed material; however, these procedures fail to demonstrate any basic difference between the various membranes forming the outer coat of the cytoplasm, the endoplasmic reticulum, the nuclear envelope, and the mitochondria. Thus, Robertson (1959) introduced the concept of the unit membrane, 75 Å thick; however, Finean (1961) found that in some specialized cell surfaces theunit membraneis thicker (100–120 Å).The bleaching procedure with strong oxidants solubilizes and removes from the sections the lower osmium oxides (Merriam, 1958) and makes it possible to evaluate a specific stainability, which otherwise would be masked by the overall staining.Marinozzi and Gautier (1961) introduced the use of phosphotungstic acid (PTA) after the bleaching procedure, and noted that the plasma membrane and some pinocytotic vesicles could be preferentially stained. More recently Latta (1962) obtained similar results, staining with PTA sections of tissues embedded in Epon.The present paper deals with the properties and the nature of the PTA stain

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02096.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

SYNOPSISObservations have been made on the fine structure of desmosomes in the rat submaxillary salivary gland. The fine structure observed has remained the same after different methods of fixation, embedding, and staining.

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02097.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb02098.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY