摘要:

SYNOPSISA technique for the removal of sheets of cellsen facefrom tissues, especially blood vessels and ducts, is described. Within the limitations of the moderate damage due to freezing and thawing, the method permits study of the enzymes of the layer so removed.

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02141.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISCarmine particles and lipids are taken up by the digestive cells of the digestive gland ofHelix. These substances are taken up by phagocytosis, and the apical and “green” granule vacuoles (Sumner, 1965) of the digestive cells are phagocytosis vacuoles. Carmine eventually becomes incorporated into the small yellow granules showing that they are derived from the “green” granules. Lipids are also taken up by the epithelia of the digestive‐gland ducts, intestine, and rectum. Phagocytosis has not been found in these sites, and the process appears to resemble that found in ve

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02142.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISClassical expositions of the theory of blood coagulation hold that consolidation of the fibrinous reticulum is due to the mechanical enmeshment of erythrocytes within it. Studies of coagulating blood by dark‐ground microscopy have revealed the attachment of submicroscopic threads to the erythrocyte walls at precise loci: it is postulated that the formation of captive cells (penderocytes) leads to haemostatic occlusion and the total retention of erythrocytes within the contracting coagulum. Filmed records of the phenomena provide distinction between true penderocytes, those erythrocytes attached at random, and occasional enmeshed cell

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02143.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISNew observations are presented on the oxyntico‐peptic cells ofGallus domesticus. In the control animals (which were starved for 72 hours) acid phosphatase is present below the nucleus in the cytoplasm of the majority of the cells lying in the upper part of the alveoli. Following the administration of histamine, there is an increase of acid phosphatase. In cells situated in the upper part of the alveoli it is present above and alongside the nucleus. In many cells lying in the lower part of the alveoli it occurs chiefly in an infranuclear position, although in some it is above and alongside the nucleus. There is a progressive decrease in the number of pepsinogen granules, and in cells in many areas of the mucosa, this is preceded by a progressive decrease in the intensity of colouring of the granules. Evacuation of the granules commences in cells lying in the lower part of the alveoli and proceeds to those that are situated in the upper part of the alveoli. The larger type of granule is discharged first, and 2 to 3 hours after injection of the drug all the granules have been discharged from the cells. It seems probable that the pepsinogen granules reappear in cells found in the basal half of the alveoli about 6 hours after histamine injection. Following the injection of pilocarpine, very large spheroids which stain an intense blue after Bowie's method are found above the nucleus in cells lying in the upper part of the alveol

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02144.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISNon‐uniformity in thickness of thin sections of wool fibres has been observed and shown to be associated with the histological structure. In the case of natural and supercontracted fibres the non‐uniformity is probably caused by shrinkage of the specimen when subjected to vacuum; in the case of wool fibres containing acrylonitrile that has been polymerizedin situit is thought to be caused by deformation effects during cutting. Any contribution from the effects of electron bombardmentin vacuois negligi

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02145.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISA general problem encountered in quantitative histology is the error caused by the inclusion of fragmented cells in the observed count from histological sections. The result of including such fragments is an overestimate of the number of cells. With this problem in mind, a method is given for estimating cell numbers in systems where cells are mononuclear with single nucleoli. The method involves applying a correction to the difference in observed counts from adjacent histological sections of different thicknesses. The proposed method is based on the same formulation of this cell counting problem as is the method of Floderus, but a major distinction, however, is that the present method does not require the estimation of the mean particle diameter or vertical height of the smallest visible fragment and thus eliminates a potential source of error.The correction formula is derived by considering a simple model for this cell counting problem. A hypothetical cell population is studied to show the theoretical equivalence of the proposed method and that of Floderus and also to show the potential bias caused by using Abercrombie's formula which does not take into account the effect of invisible fragments. The new method is compared with other available techniques and is empirically evaluated by extensive counting in a human superior cervical sympathetic ganglion.

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02146.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISThe Kerr‐effect microscope is of great utility in the static and dynamic investigation of magnetic domain patterns in thin films used for computer storage. An account is presented of the theoretical considerations which decide the optimum instrumental configuration for maximum contrast and this leads to two microscope designs: one using a single lens, and the other, two lenses. The type of domain shown by these microscopes is illustrate

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02147.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISThe passage of coloured chelates derived from 3:4:7:8‐tetramethyl‐1:10 phenanthroline and divalent copper and ruthenium into Landschütz ascites tumour cells,in vitroand in the mouse, has been examined by bright‐field and fluorescence microscopy. Chelate absorption on to the cell surface occurs rapidly but penetration into the cell appears to be a slow process and is generally associated with cell damage.It has been shown by the use of the vital stain lissamine green that exposure to metal chelate decreases significantly the viability of Landschütz ascites tumour bothin vitroand in th

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02148.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISMethods are described for staining cells of the nervous system using sodium and potassium iodate instead of the carbonate salts as used in the classical Hortega techniques. The methods are quick and easy, the rate of success high, and tissues which have been fixed for a long time in formol can be used.

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02149.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY

摘要:

SYNOPSISA histochemical method for the demonstration of fructose‐1–6‐diphosphate aldolase and fructose‐1‐phosphate aldolase is presented. The method, which incorporates the requirements for the biochemical estimation of these enzymes, has been applied to various fresh frozen tissues including a liver biopsy from a case of fructose intolerance. The results show that in fructose intolerance both these enzymes were reduced in amount, a finding which was confirmed by biochemical e

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1965.tb02150.x

出版商:Blackwell Publishing Ltd    

年代:1965

数据来源: WILEY