ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05293.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05294.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

SYNOPSISThe survey describes the various embedding media that are now available for thin sectioning and electron microscopy, with the aim of aiding a correct choice of medium in any particular investigation. The properties of the methacrylates, the epoxy resins and the polyester resins are compared and, in particular, the basic similarities between the different mixtures containing epoxy resins are emphasized. A description of water‐soluble embedding media is include

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05295.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

SYNOPSISOn comparing the electron‐microscope appearance of, and the sites of tritiated thymidine uptake within, the nuclei of cartilage cells in the growing epiphysis, a corresponding pattern of distribution of electron‐dense material and isotope was found. Similarly, cells capable of tritiated thymidine uptake, and in which metaphase arrests can be produced with colchicine, were found to have electron‐dense nuclei on examination in the electron microscope. When the pattern of tritiated thymidine was changed by the prior administration of growth hormone to the animal, the distribution of electron‐dense nuclei showed the same changes.During the active phase, direct physical connections were observed to exist between the nuclei and surroundings of the cartilage cells. The effect of vitamin C deficiency was to inhibit the formation of endoplasmic reticulum, while leaving the connections between the nucleus and exterior of the cell intact.The main effect of colchicine was to remove the connections between the nucleus and its surroundings, while the nucleolus was frequently enlarged. (The central part of the nucleolus is normally connected directly with a large cytoplasmic inclusion.) Individual chromosomes are rarely seen in cartilage, even after the administration of colchicine, although after this treatment chromosomes were frequently found in marro

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05296.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

SYNOPSISCoal, coke and some other carbonaceous materials present special difficulties in both thin sectioning and polishing, whether the sample is granular or in lump form. Improved techniques using polyester resin have been worked out, and have been found more reliable and convenient than other methods of thin sectioning and polishing. Large parallel‐sided thin sections, wholly enclosed in plastic, are useful for studying and demonstrating comparatively gross structures, but may also be used at high magnification to study fine detail. Methods of preparing sections and surfaces of very small amounts of material (i.e. down to 0·1 mg or even less) are described, also the use of vacuum embedding and impregnati

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05297.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05298.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05299.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

SYNOPSISAn improvement in the speed and accuracy with which ultra‐thin sections can be mounted upon formvar coated grids is possible by means of a simple device. The greater degree of precision with which sections can be placed upon the formvar film enables the steel slot type grid to be used with a greater degree of success in histological investigation

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05300.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

SYNOPSISA fibre microtome is described which combines the simplicity of the plate method of sectioning with the accuracy and efficiency of the Hardy fibre microtome. The instrument is cheap to make, uses only standard materials and may be constructed by anyone having access to a small lathe and simple hand tools.

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05301.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1962.tb05302.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY