摘要:

SYNOPSISAn assembly of equipment for the study of living human cells is described. It is designed to keep the cells under study living, sterile, adequately nourished, at their optimum temperature and observable throughout an experiment, and to subject them to as little light as possible. The apparatus consists of a phase‐contrast microscope, a cine camera, a micro‐flash unit, a time‐lapse unit, and a warmed perfusion chamber. The microscope, camera, and flash unit are standard, commercially available items. The time‐lapse unit, its ancillary equipment, and the perfusion chamber are of the author's

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00491.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

SYNOPSISIn muscle fibres of the gastrocnemius of adult rabbits and rats, denervated for at least one month, electron microscopy revealed peculiar lamellated structures. Some of these appeared as arrays of parallel double lamellæ; others folded themselves into ovoids or asterisk‐like patterns, while still others were of irregular shape.One to three months after denervation, the outer membrane of many muscle nuclei protruded to a varying extent, and later the protrusion folded into an array of parallel double lamellæ. When the array reached a certain size, it became detached from the nuclear membrane and assumed various forms. Such structures were more numerous in muscles denervated for longer than 3 months. They seemed to move gradually toward the sarcolemma and to become finally attached to it or to fuse with it. These findings support the current view that all membrane‐bounded cytoplasmic organelles belong to a common “membrane system” extending from the plasma membrane to the nuclear membrane. It is concluded that the lamellated structures in denervated muscle are derived from the outer nuclear membrane and that they are an outcome of the degeneration of musc

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00492.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00493.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

SYNOPSISThe histological structure is described, for the first time, of certain of the viscera of the Sumatran Rhinoceros (Didermocerus sumatrensis).

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00494.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

SYNOPSISUsing the Dyson image‐splitting eyepiece, a careful unfatigued observer can measure the dimensions of a bacterium with a standard deviation of about 0.03 μ. Systematic as well as random errors are discussed. The application of the eyepiece is illustrated by a number of specialized examples, including the growth of individual bacter

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00495.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

SYNOPSISThe carcinostatic action of phenazine‐di‐N‐oxide against the Ehrlich tumour was subjected to closer study on individual cells. A method was devised whereby the increase in cell diameter could be followed by direct measurement on the projected microscope image. After standardization of the optics so that 1 μ on the slide equalled 1 mm on the ruler, 50 cells per 20 minutes could be measured randomly.Observations 24 hours after drug administration into a mouse bearing a 7‐day tumour showed that treated cell diameters increased from a median diameter of 14 μ to a median of 21 μ (many had doubled in size) as compared to an increase of 1.9 μ in the controls. After 72 hours the treated cells reached a maximum diameter and disintegrated. By this time there was an absence of ascitic fluid. The drug acted byacceleratingthe growth andexaggeratingthe disordered mitoses of the tumour cells which were unable to cleave daughter cells, leaving a cluster of partially divided pr

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00496.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00497.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

SYNOPSISA reconstruction of a simple system for viewing the ring and brush patterns produced by crystals in convergent light, originally manufactured by Powell and Lealand, is shown to have an equivalent in terms of modern components which is simple to construct.

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00498.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY

摘要:

Book reviewed in this article:Division of Labor in Cells. Geoffrey H. Bourne.Electron Microscopy.British Medical Bulletin.

ISSN:0368-3974    

DOI:10.1111/j.1365-2818.1963.tb00499.x

出版商:Blackwell Publishing Ltd    

年代:1963

数据来源: WILEY