摘要:

AbstractMelanoma‐specific T‐cells (CTLs) are specifically cytotoxic for autologous tumor, when assayed in vitro. To examine their effectiveness in vivo, we tested the ability of these human T‐cells to inhibit growth of human melanoma xenografts by using a Winn assay. Nude mice receiving specific CTLs (n = 10) demonstrated a dramatic inhibition of tumor growth. All treated mice were tumor‐free at day 50 and nine remained tumor‐free at day 65, vs. control mice (n= 10) with average tumor volumes of 321 mm3and 808 mm3, respectively. To control for the possibility that a non‐specific response to the human T‐cells could inhibit tumor growth, an additional group received allospecific CTLs. There was no inhibition of tumor growth in this group (n =8), with the average tumor volume of 2,768 mm3at day 40 vs. 1,882 mm3in the control group (n = 10). We conclude that these tumor‐specific CTLs can inhibit tumor growth in vivo and may prove useful in the adoptive immunother

ISSN:0022-4790    

DOI:10.1002/jso.2930430202

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractPhenotypic characteristics of endothelial cells of blood vessels in renal cell carcinoma were studied immunohistochemically. Vascular endothelial cells in different compartments expressed surface antigens heterogenously. Sinusoidal capillary endothelial cells adjacent to carcinoma cells shared antigens with a monocyte/macrophage subset (OKM5 +, HLA‐DR +, interleukin‐1 +), but were only faintly positive or absent for factor VIII/von Willebrand factor antigen. Endothelial cells of larger vessels in fibrous septa were OKM5‐, HLA‐DR +, interleukin‐1 +, factor VIII/von Willebrand factor antigen+. Renal cell carcinoma cells expressed HLA‐DR and HLA‐ABC antigens. These findings suggest that renal cell carcinoma cells are surrounded by blood vessels containing effector cells for immune responses, and that they have a high‐potency response to imm

ISSN:0022-4790    

DOI:10.1002/jso.2930430203

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractTissue activity levels of ornithine decarboxylase (ODC) have been suggested to be a marker of colonic epithelial cell proliferation and risk for colorectal cancer. Supplemental dietary calcium has been hypothesized and reported to suppress colonic epithelial‐cell proliferation. We measured sigmoid and rectal mucosal ODC activity levels in 45 healthy, disease‐free subjects with strong family histories of colorectal cancer before and after 2 months, during which daily dietary supplementation with calcium carbonate (to provide 600 mg calcium base) was taken. Although the mean ODC activity levels decreased in both sigmoid and rectal specimens, these changes were small in relation to the standard deviation and were not statistically significant. These data suggest that the magnitude of dietary calcium intake does not significantly influence sigmoid‐rectal mucosal ODC activity levels and that ODC measurements may not be a useful intermediate endpoint for interventions designed to interrupt the colon carcinogenic seq

ISSN:0022-4790    

DOI:10.1002/jso.2930430204

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractA feasibility study of the treatment of advanced superficial human malignant tumors utilizing direct intralesional injections of cisplatin mixed with purified bovine collagen was performed. The purpose of using intralesional injection of cisplatin mixed with collagen was to limit the drug exposure to normal tissues while increasing the dose and duration of exposure to the tumor. Fourteen evaluable superficial tumors in four patients (melanoma, breast CA, squamous CA from larynx) received a total of 65 treatments in the outpatient clinic setting. All patients had failed prior treatment with systemic intravenous cisplatin. Lesions were treated at least three times at two‐week intervals. After intramuscular meperidine premedication, multiple injections of cisplatin mixed with collagen were made into the tumors. There was minimal normal tissue toxicity and minimal systemic toxicity. Tumor regression or stabilization occurred in 86% (12/14) of tumors; 50% (7/14) of lesions regressed more than 50% in size. This study suggests that intralesional colloidal cisplatin can overcome resistance to systemic intravenous cisplati

ISSN:0022-4790    

DOI:10.1002/jso.2930430205

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractWith the popularity of breast‐conserving treatment plans, the natural history of “breast recurrence” in the ipsilateral breast must be distinguished from local recurrence following modified radical mastectomy. Hence, this study considers those patients who develop skin or chest wall recurrence after modified radical mastectomy, whether as a primary procedure or for patients with “breast recurrence” after partial mastectomy. The incidence of postmastectomy locally recurrent breast cancer following modified radical mastectomy (MRM) and adjuvant immunotherapy (IT) is compared to historical controls. The risk factors and treatment of local recurrence in this program as well as in a larger group of patients who recurred after modified radical mastectomy are

ISSN:0022-4790    

DOI:10.1002/jso.2930430206

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractThe majority of cancer patients at the terminal stage experience pain as a major symptom, and less than 60% of patients receive adequate pain relief. To relieve this symptom we have placed epidural catheters and attached them to an implantable access system. On this method the patients required 0.4–3.5 mg of morphine per hour with occasional boluses. Catheter life span ranged from 15 to 157 days with an average of 57 day

ISSN:0022-4790    

DOI:10.1002/jso.2930430207

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractFibronectin (FN) was investigated in 75 stroma from lung adenocarcinoma patients. Distribution of FN was classified into three patterns based on immunohistochemical evidence: dense, moderate, and sparse distribution. The incidence of the sparse pattern in both stage I and stage III disease was significantly greater in the infiltrating than in the noninfiltrating areas. The patients were divided into two groups, long‐term survivors and short‐term survivors. In the group with stage I disease, the incidence of the sparse pattern was significantly greater in the infiltrating than in the non‐infiltrating areas. In both areas, the incidence of sparse pattern was greater in the short‐term than in the long‐term survivors. These data suggest that the disappearance of FN reflects the aggressiveness of the tumor and presumably plays an important role in the prognosis of adenocarcinoma of

ISSN:0022-4790    

DOI:10.1002/jso.2930430208

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractPathological sections of gastrectomized specimens of 74 patients with benign gastric ulcer and 79 with gastric cancer were reviewed. Intestinal metaplasia was found in 26 specimens with benign ulcer (35.1%) and in 43 with cancer (54.4%), a difference that is statistically significant. Further analysis of age groups showed that rate of occurrence of metaplasia in cancer patients older than 60 years was 70.3%, which was significantly higher than that of their younger counterparts (40.5%) and of patients with benign ulcer of either age group. A survey was also conducted by taking biopsies of mucosa of antrum and body of the stomach of 250 patients who underwent gastroscopic examinations. Acute and chronic gastritis was found in 22 and 156 patients, respectively. Intestinal metaplasia was found to be associated with acute gastritis in 2 (9.1%) and with chronic gastritis in 25 (16%) patients. In conclusion, intestinal metaplasia was associated with gastric cancer in patients who were older than 60 years in a significantly higher proportion than it was with benign gastric ulcer and gastritis among Thai patients.

ISSN:0022-4790    

DOI:10.1002/jso.2930430209

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractThirty cases of colorectal adenocarcinoma were immunohistochemicaliy examined to clarify the relationship between the degree of differentiation of cancer cells reflected by the mode of CEA localization and the infiltration pattern of mononuclear cells in the carcinoma tissues. Leu 1+, Leu 2a+, Leu 3a + 3b+T lymphocytes, Leu 7+cells, and IgA‐containing plasma cells tended to be distributed more in areas where CEA was localized over the entire surface of the cancer cells or in their surrounding stroma than where CEA was located along the apical surface or within the cytoplasm. Carcinoma cells with the pattern of CEA staining at the former site were previously shown to be less differentiated than those at the latter. There were also CEA‐containing phagocytes which were often observed where T cells and IgA plasma cells had aggregated. We speculated that the dedifferentiation of cancer cells might cause the host immune response, especially the cellular immunity, in malignant lesi

ISSN:0022-4790    

DOI:10.1002/jso.2930430210

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY

摘要:

AbstractTILs can be isolated and expanded in vitro in the presence of RIL‐2. The resulting cells are highly cytotoxic in vitro and in vivo against a variety of tumor targets. Although most of the TILs bear T cell antigens on their cell surface, recent evidence suggests that natural killer (NK) cells may be part of the overall population of infiltrating cells. Based upon this evidence, we have evaluated the effects of Picibanil (OK‐432), a well‐known inducer of NK cell and T cell cytotoxicity, on TILs. OK‐432 was administered intravenously at a dose of 100 μg, previously determined to be optimal for NK stimulation, to tumor‐bearing animals. Two days later, control and experimental animals had their tumors harvested and processed in order to grow their TILs in vitro in complete medium containing RIL‐2 at a final concentration of 1,000 U/ml. The following observations were made: 1) a greater than 300% increase in overall TIL number compared to controls on day 10 of culture returning to normal by day 30; 2) a marked increase in the percent of cells expressing cytotoxic and differentiation antigens in the experimental group compared to controls, such increase seen mostly from day 7 to day 10; 3) a marked increase in the cytotoxic activity of the experimental TILs against an NK‐sensitive tumor target, the YAC‐1 lymphoma, throughout the period of growth of the TILs (3–4 times controls) and to a lesser extent against an NK‐resistant tumor target. These findings may have potential application in immunotherapeutic trials against human tumors and may help to understand the reasons for the effectiveness of OK‐432 in vivo against

ISSN:0022-4790    

DOI:10.1002/jso.2930430211

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1990

数据来源: WILEY