摘要:

AbstractThe correlation between DNA ploidy pattern and clinical outcome was studied in 76 patients with Borrmann type 4 gastric carcinomas. Twenty‐six tumors were diploid, and 50 tumors were aneuploid. There was no correlation among DNA ploidy and histologic type, lymph‐node status, wall invasion, or clinical stage. The incidence of vascular invasion in the aneuploid tumors was significantly higher than that in the diploid tumors. Five year survival was achieved in 28% of the patients with diploid tumors and 8% of those with aneuploid tumors, respectively. Among the patients undergoing curative resection, 5 year survival rate was 54% in the patients with diploid tumors compared to 28% with aneuploid tumors. There was a significant survival advantage in patients with diploid tumors. These results indicate that DNA ploidy might be an important prognostic factor in Borrmann type 4 gastric carcino

ISSN:0022-4790    

DOI:10.1002/jso.2930420103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThe purpose of the present study was to evaluate the therapeutic effects of hyperthermia associated with radiotherapy on neoplasia. Two transplanted experimental tumors (undifferentiated carcinoma of mouse breast and sarcoma 37) were used. A protocol was followed that included, for both models, four groups of animals: 1) control group; 2) radiotherapy group; 3) hyperthermia group; 4) radiotherapy associated with hyperthermia group. The animals were sacrificed after therapy according to a different schedule for each type of tumor. The morphology of the neoplasia in every group of treated tumors was compared with that of the control group. For quantitative evaluation of the necrosis, we studied the ratio of the tumor necrotic areas (N) to a tumor standard area (T). From the results obtained, the following conclusions were made: 1) Necrosis produced by combined treatment was significantly greater than that obtained by using one only; 2) necrosis appeared early after treatment and remained relatively unchanged; 3) The damaging effect of the hyperthermia occurred earlier than that produced by radiotherapy when these methods were used separately, thus suggesting distinct mechanisms of tumor necrosis.

ISSN:0022-4790    

DOI:10.1002/jso.2930420104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractSix patients with metastases to the penis are described. The main characteristics of the primary tumors are described, along with the treatments, the features of the metastasis, and the response to treatment. Although in one patient a survival of 5 years was obtained, metastasis to the penis is generally associated with a dismal prognosis. A brief commentary is made concerning mechanisms of metastases formation.

ISSN:0022-4790    

DOI:10.1002/jso.2930420105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractLung cancer tissues from 68 patients were examined for epidermal growth factor (EGF) receptor levels and EGF receptor gene copy numbers. Histologic cell types of these lung cancer tissues included squamous‐cell carcinoma (n = 30), adenocarcinoma (n = 28), large‐cell carcinoma (n = 4), and small‐cell carcinoma (n = 6). Tissues of squamous‐cell carcinoma exhibited exceptionally high125I‐EGF binding activity, and those of small‐cell carcinoma showed no EGF binding activity. Southern blot hybridization analysis revealed EGF receptor gene amplification in the squamous‐cell carcinomas with high EGF binding activity. The EGF receptor levels in squamous‐cell carcinomas and adenocarcinomas were compared with their pathological staging grouping and pathological findings, including degree of differentiation, diameter of tumor, and lymph node metastasis. However, unlike previous reports on breast and bladder cancers, there was no obvious correlation between these pathological characteristics and the EGF receptor levels

ISSN:0022-4790    

DOI:10.1002/jso.2930420106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThirty‐seven of 54 human squamous head and neck carcinomas were successfully grown as first transplant generation xenografts under the kidney capsule of conventional mice immunosuppressed by daily treatment with 60 mg/kg of cyclosporine. Of the 18 different tumors evaluated for chemosensitivity, 39% responded to cis‐platinum, 19% to 5‐fluoro‐uracil, 33% to methotrexate, and 40% to Cis‐Pt/5Fu. In comparing the assay results in nine patients who received the same chemotherapeutic drugs, two of three patients responded to their drug with xenograft shrinkage noted, whereas four of six patients who did not respond had tumor growth in the mouse. It is hoped that this model will become useful for new drug testing and, in certain cases, for selection of chemotherapy for patients with refracto

ISSN:0022-4790    

DOI:10.1002/jso.2930420107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractTumor cells from 40 patients were tested by MTT assay. Five thousand to sixteen thousand tumor cells were plated into 96‐well microplates with various concentrations of anticancer agents. After incubation for 48 h, the absorbance of each well was detected with an EIA reader and the effects of the agents were evaluated as positive when the inhibition rate was equal to or more than 50%. Normal cells were also processed under the same conditions, and the absorbance for normal cells was lower than that of tumor cells with statistical significance (P<.05). The efficacy rates of MMC, 5‐FU, ADM, and CDDP were 16.7, 8.3, 13.9, and 5.6%, respectively. The overall accuracy of this assay for clinical effects was 83.3%, with one false‐positive and five true‐negative cases. This MTT assay was also assumed to be useful for in vitro chemosensitivity testing of fresh surgical sp

ISSN:0022-4790    

DOI:10.1002/jso.2930420108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractRecent advances in the diagnosis and treatment of patients with bladder cancer include flow cytometry, intravesical bacillus Calmette‐Guerin (BCG), and systemic chemotherapy. Once the subjects of clinical research protocols, these modalities are currently being incorporated into the general management of patients with bladder cancer. Flow cytometry, intravesical BCG, and systemic chemotherapy will be individually reviewed and their role in the management of patients with bladder cancer will be discusse

ISSN:0022-4790    

DOI:10.1002/jso.2930420109

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractFour monoclonal antibodies (MoAbs) reactive with carcinoembryonic antigen (CEA) were obtained by hybridizing mouse myeloma cells (P3‐X63‐Ag8‐U1) with spleen cells from nude mice (BALB/c, nu/nu) that had rejected transplanted human colonic adenocarcinomas Co‐3 and Co‐4 following intraperitoneal injection of spleen cells from immunocompetent mice (BALB/c). By solid‐phase RIA with purified CEA and its related antigens, NCC‐CO‐413 (IgG2a, kappa) was shown to react with NCA and BGP‐I as well as with CEA, whereas the reactivities of three other MoAbs, NCC‐CO‐308 (IgG1, kappa), ‐432 (IgG1 lambda), and ‐411 (IgG1, kappa) were limited to CEA. Immunohistochemical reactivities of these MoAbs to colonic carcinomas, granulocytes, and liver bile canaliculi on acetone‐fixed paraffin‐embedded sections (“AMeX” sections) confirmed the specificities of these MoAbs shown by the solid‐phase RIA. By competition solid‐phase RIA, the epitopes recognized by NCC‐CO‐308 and ‐432 were shown to be shared or located close to each other, whereas the other MoAbs were shown to recognize different epitopes. Thus, two epitopes specific to CEA and one shared by NCA and BGP‐I as well as CEA were identified. Furthermore, reactivities of MoAbs with the two CEA‐specific epitopes were easily abolished by heat denaturation or reduction of CEA, as revealed by solid‐phase RIA and SDS‐PAGE‐im‐munoblotting, indicating that these two CEA‐specific epitopes are

ISSN:0022-4790    

DOI:10.1002/jso.2930420110

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractNinety‐three patients with nodal metastases from melanoma (stage II) located in the head and neck underwent surgery at the National Cancer Institute of Milan. Different surgical techniques were employed, ranging from radical to conservative treatment. Analysis of the data shows no significant difference from an oncological standpoint between radical and conservative surgery when a radical dissection is performed. Elective nodal dissections for malignant melanoma of the head and neck region, like those at other sites of lymphatic drainage such as the groin and axilla, did not prove beneficial. We do recommend parotidectomy in cases where the primary tumor arises in the superior area of the head. The number of nodes involved and the type of disease spread constitute the major prognostic factors, as in the case of melanomas located in other sites. Our data further indicate that the incidence of distant and local recurrence is not influenced by the type of dissection performe

ISSN:0022-4790    

DOI:10.1002/jso.2930420111

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractTumors attached or adjacent to critical structures can often not be completely resected or resected with adequate surgical margins. Sites involving major blood vessels, the paravertebral spaces, or critical abdominal structures often present technical difficulties for standard brachytherapy procedures using 1‐125 or Ir‐192 implants. These techniques allow for a high‐dose delivery to the tumor bed with minimal normal tissue toxicity. A relatively simple and accurate method is described using 1‐125 seeds in Vicryl® suture threaded through Gelfoam®. These permanent implant procedures with radioactive 1‐125 seeds effectively treat small residual tumors or suspicious margins where standard brachytherapy techniques may be unsatisfactory and technically difficult

ISSN:0022-4790    

DOI:10.1002/jso.2930420112

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY