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11. |
In vitrocytotoxicity of a glass ionomer cement of a new generation |
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Cell Biochemistry and Function,
Volume 2,
Issue 1,
1984,
Page 43-48
S. D. Meryon,
R. M. Browne,
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摘要:
AbstractThe new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Testing of set samples of the material showed that its toxicity decreased rapidly with setting. ChemFil behaved in a manner similar to two older glass ionomers, ASPA and ChemBond, although it was generally slightly less irritant.
ISSN:0263-6484
DOI:10.1002/cbf.290020112
出版商:John Wiley&Sons, Ltd.
年代:1984
数据来源: WILEY
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12. |
Studies on rat liver mitochondria:4. Enzyme activities in mitochondria preserved at 0—4°C |
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Cell Biochemistry and Function,
Volume 2,
Issue 1,
1984,
Page 49-52
Patricia Teixeira Padilha da Silva,
Ilma Hiroko Higuti,
Mario Stencel,
Annibal de Paiva Campello,
Aguinaldo José do Nascimento,
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摘要:
AbstractRat liver mitochondria, stored with the energy‐linked functions preserved or in aging conditions, were used to assay the activity of various enzymes during five days. The preservation of energy‐linked functions was monitored by the respiratory control coefficient. ATPase, cytochrome oxidase and NADH dehydrogenase showed increased activity when the energy‐linked functions were preserved. In aging conditions, cytochrome oxidase, NADH dehydrogenase and ATPase showed decreased activity. The ATPase activity increased only when mitochondria were stored in the presence of inhibitors of the electron transport chain. The activity of NADH oxidase did not change, and succinate oxidase and succinate dehydrogenase showed a small decrease in their activity. The enzymes of the matrix, α‐ketoglutarate dehydrogenase, malate dehydrogenase and aspartate aminotransferase showed little decrease in activity under either of the conditions of storage. The total protein content decreased slightly under both conditions of storage. These results show that the activity of the enzymes analysed was maintained at reasonable levels, when the energy‐linked functions of isolated mitochondria were
ISSN:0263-6484
DOI:10.1002/cbf.290020113
出版商:John Wiley&Sons, Ltd.
年代:1984
数据来源: WILEY
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13. |
Glycosaminoglycan: Cell interactions; their role in lipoprotein lipase secretion from isolated cardiac muscle cells |
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Cell Biochemistry and Function,
Volume 2,
Issue 1,
1984,
Page 53-56
Anthony Cryer,
Frederick S. Wusteman,
Jill J. Casey,
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摘要:
AbstractWhen cardiac muscle cells from mature rats were incubated in vitro in the presence of heparin (8.7 nmole ml−1) lipoprotein lipase activity appeared in the incubation medium. The intracellular activity of the enzyme remained unchanged. Other glycosaminoglycans (heparan sulphate, dermatan sulphate, keratan sulphate and chrondroitin 6‐sulphate) at the same or higher concentrations were totally ineffective in producing any enzyme redistribution between cells and medium. The release seen in the presence of heparin was blocked by the presence of cycloheximide. Cycloheximide by contrast had no effect on the release observed in the presence of dexamethasone, The action of endogenous glycosaminoglycans are unlikely therefore to have a significant role to play in the movement of lipoprotein lipase in heart tissuein v
ISSN:0263-6484
DOI:10.1002/cbf.290020114
出版商:John Wiley&Sons, Ltd.
年代:1984
数据来源: WILEY
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14. |
Use of microscopic interferometry for measuring changes in water content of small samples of tissue |
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Cell Biochemistry and Function,
Volume 2,
Issue 1,
1984,
Page 57-61
S. Darracott‐C̄anković,
M. V. Braimbridge,
K. Kyösola,
L. Bitensky,
J. Chayen,
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摘要:
AbstractOedema following periods of ischaemic arrest and subsequent reperfusion has been shown experimentally and clinically to affect the functional state of the heart. Tissue water content has been measured in myocardial sections by microscopic interferometry and densitometry, and the results correlated with those obtained by wet and dry weight analysis (r= 0.87;p<0.001). Microscopic interferometry also revealed the distribution of the water in the tissue. Experimentally induced ischaemic arrest in isolated rat hearts resulted in predominantly intra‐fibrillar oedema, whilst subsequent reperfusion resulted in interfibrillar oedema. Microscopic interferometry facilitates accurate measurement of water content in tissue samples as small as 2 mg wet weight and shows (as conventional wet/dry weight analysis cannot) the distribution of the water in the tissu
ISSN:0263-6484
DOI:10.1002/cbf.290020115
出版商:John Wiley&Sons, Ltd.
年代:1984
数据来源: WILEY
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15. |
Protein purification: Principles and practice |
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Cell Biochemistry and Function,
Volume 2,
Issue 1,
1984,
Page 63-63
C. R. Lowe,
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ISSN:0263-6484
DOI:10.1002/cbf.290020117
出版商:John Wiley&Sons, Ltd.
年代:1984
数据来源: WILEY
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16. |
Calendar |
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Cell Biochemistry and Function,
Volume 2,
Issue 1,
1984,
Page 64-64
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ISSN:0263-6484
DOI:10.1002/cbf.290020118
出版商:John Wiley&Sons, Ltd.
年代:1984
数据来源: WILEY
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17. |
Masthead |
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Cell Biochemistry and Function,
Volume 2,
Issue 1,
1984,
Page -
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PDF (101KB)
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ISSN:0263-6484
DOI:10.1002/cbf.290020101
出版商:John Wiley&Sons, Ltd.
年代:1984
数据来源: WILEY
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