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1. |
Tellurite effect on ATPase activity of contractile membrane protein |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 227-230
Libuše Mirčevová,
Milan Kodíček,
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摘要:
AbstractThe effect of tellurite on ATPase activity of the contractile membrane protein in human erythrocytes was studied. Tellurite, even at a concentration of 0·01 mM, inhibited 25 per cent of the saponin‐stimulated ATPase activity of the contractile membrane protein; the inhibition increased with increasing tellurite concentration, and was reversible. On the other hand, in erythrocytes preincubated with tellurite, the ATPase activity of the membrane contractile protein, non‐stimulated by saponin, increased, and the increase was further enhanced by washing the erythrocytes. The behaviour is analogous to the tellurite effect on erythrocyte morphology: incubation of erythrocytes with tellurite caused morphological changes which were more pronounced after removing the tellurite by washing. The complex effect of tellurite is discu
ISSN:0263-6484
DOI:10.1002/cbf.290090403
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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2. |
Differential effects of unsaturated fatty acids on modulation of endotoxin‐induced tissue factor activation in cultured human leukemia U937 cells |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 231-238
Arthur J. Chu,
John Moore,
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摘要:
AbstractWe examined the direct effects of unsaturated fatty acids, oleic (18:1 n‐9), linoleic (18:2 n‐6), eicosapentaenoic (20:5 n‐3) and docosahexaenoic (22:6 n‐3) on tissue factor (TF) activity in the human leukemia monocytic U937 cell line. After exposing cells to fatty acids for 16 h, there were no significant effects on either TF activity or its activation induced by bacterial endotoxin (LPS). When the cells were primed with fatty acids for 24 h, 48 h or 72 h, the TF activity remained essentially unchanged. However, the extent of TF‐activation induced by LPS depended on the length of priming, and the dose and the degree of unsaturation of the fatty acids to which cells were exposed. After a 72‐h priming, 18:1 produced 40–60 per cent elevation in LPS‐challenge. In contrast, approximately 20–50 per cent reduction in LPS‐challenge was achieved by 18:2, 20:5 and 22:6 at high concentrations. The results suggest that chronic exposure of U937 cells to unsaturated fatty acids leads to modulation of the TF‐activati
ISSN:0263-6484
DOI:10.1002/cbf.290090404
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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3. |
Reduced neutrophil superoxide generation and plasma lipoperoxide levels in pigs fed with cod liver oil |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 239-243
Carlo Guarnieri,
Li You‐Zhou,
Elizabeth Roth,
Pierluigi Biagi,
Giovanni Ussia,
Elisabetta Baccarini,
Francesco Farruggia,
Gary L. Anderson,
John H. Barker,
Giovanni Galletti,
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摘要:
AbstractCirculating neutrophils, isolated from pigs fed for 8 weeks with a diet supplemented with CLO, had an accentuated n‐3 fatty acid incorporation into the plasma membrane, as evidenced by an approximately four‐fold greater n‐3/n‐6 ratio as compared with the control diet group. Moreover, the neutrophils of the CLO fed pigs produced less superoxide when stimulated with PMA or f‐MLP, as well as showing a more prolonged latency period before O2−‐generation. In the plasma of pigs fed with CLO there were higher levels of thiobarbituric reactive material and lipofuscin, while the content of GSSG was similar in both dietary groups.The results of this study indicate that dietary supplementation with CLO reduces the activation of circulating neutrophils and favours the presence in the plasma of
ISSN:0263-6484
DOI:10.1002/cbf.290090405
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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4. |
The effect of ligands on the uptake of iron by cells in culture |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 245-253
S. K. Jonas,
P. A. Riley,
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摘要:
AbstractUptake of iron by a mammalian epithelial cell line (CNCM I‐221) was shown to be dependent on the nature of the iron complex. Iron uptake was demonstrated by cytochemical staining and determination of redox‐reactive iron in cell lysates. Three classes of ligands were investigated: (i) low molecular weight hydrophilic compounds, represented by ethylenediamine‐tetraacetic acid (EDTA) and other charged ligands such as adenosine phosphates (ATP, ADP, AMP) and diethylenetriaminepentaacetic acid (DTPA), (2) low‐molecular weight lipophilic ligands such as 8‐hydroxyquinoline (8‐HQ) and (3) a high molecular mass ligand, dextran. Iron complexed to 8‐HQ accumulated intracellularly, the uptake rate of iron being 4·16 fmoles cell−1h−1of exposure at 37°C or 3·86 fmoles cell−1h−1at 4°C. Iron‐dextran was endocytosed and retained in phagosomes. The uptake rate of iron following exposure to iron dextrans was found to be 5°6 fmoles cell−1h−1of exposure at 37°C. In contrast to iron/8‐HQ, uptake of iron dextran by cells was inhibited at 4°C. Iron complexed to low molecular weight hydrophilic ligands was not taken up by cells.Cytotoxicity was measured by reduction of plating efficiency or tritiated thymidine incorporation. These tests showed that toxic effects of added iron were demonstrable only in ce
ISSN:0263-6484
DOI:10.1002/cbf.290090406
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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5. |
Relation between hyaluronan and sulphated glycosaminoglycan synthesis and degradation in cultured embryonic fibroblasts. Effect of concanavalin a and ammonium chloride administration |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 255-262
P. Carinci,
P. Locci,
R. Evangelisti,
L. Marinucci,
L. Rossi,
E. Becchetti,
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摘要:
AbstractIn order to evaluate the relationship between glycosaminoglycan (GAG) synthesis and degradation, the effect of NH4Cl, which inhibits lysosomal degradation, on GAG production was analysedin vitroin concanavalin A (Con A) stimulated fibroblasts from 7 and 14‐day‐old chick embryos.35SO4incorporation into total proteoglycan (PG),3H incorporation into individual GAG classes, β‐N‐acetyl‐D‐glucosaminidase and β‐D‐glucuronidase activity were determined. The results indicate a correlation between Con A and NH4Cl effects: NH4Cl induced a reduction principally in the GAG classes most stimulated by Con A. Thus HA and DS are much more stimulated by Con A and inhibited by NH4Cl t
ISSN:0263-6484
DOI:10.1002/cbf.290090407
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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6. |
Phorbol myristate acetate inhibits phosphoinositol lipid‐specific phospholipase C activity via protien kinase C activation in conditions inducing differentiation in HL‐60 cells |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 263-273
Hélène Cost,
Patricia Barreau,
Michèle Basset,
Christian Le Peuch,
Blandine Geny,
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摘要:
AbstractWe have studied, in streptolysin O‐permeabilized HL‐60 cell membrane preparations, the effects of phorbol 12‐myristate 13‐acetate (PMA) on polyphosphoinositide‐specific phospholipase C (PLC) activity and on terminal differentiation towards macrophagic‐like cells. We showed that terminal differentiation was induced when differentiating concentrations of the drug were present for only 1–2 h in the culture medium. Conditions inducing differentiation also inhibited PLC activity for a long lasting period (at least 5 h). When terminal differentiation affected only part of the cell population, inhibition of phospholipase C activity was found to be less marked and reversible over the period studied. Moreover in experiments done in an HL‐60 clone resistant to PMA, no inhibition of PLC activity was provoked by this tumour promotor.In order to study the involvement of protein kinase C in this process, we measured modifications of PLC activity by PMA in the presence of two different protein kinase C inhibitors, staurosporine and H‐7. They both prevented the inhibition of PLC activity by PMA indicating that this inhibition is likely to be related to the effect of PMA on protein kinase C activity. This was also confirmed by the fact that active protein kinase C, by itself, was able to decrease PLC activity when added to membrane preparations or to streptolysin O‐permeabilized control HL‐60 cells.These results indicate that PMA acts in inhibiting phospholipase C activity through its effect on protein kinase C activation and/or on protein kinase C translocation to the plasma membrane and that terminal differentiation, might be related to changes in both protein kinase
ISSN:0263-6484
DOI:10.1002/cbf.290090408
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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7. |
Hepatic selective adjustments in short‐term cold exposed rats |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 275-280
S. Iossa,
A. Barletta,
G. Liverini,
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摘要:
AbstractThe metabolic adjustments which occur in rat liver cells during the first days of cold exposure have been investigated. We have measured both mitochondrial mass and oxidative capacities as well as ATP production after five and 10 days of cold exposure. In addition, we have measured plasma membrane Na+/K+‐ATPase activity. Cold exposure elicited a significant increase in mitochondrial mass as well as in state 3 oxidative rates and ATP production in isolated mitochondria using various substrates. Moreover, our results show that Na+‐pumping activity significantly increased after both five and 10 days of cold exposure.Our results suggest that during the first days of cold exposure liver cells undergo alterations which are useful for survival in the c
ISSN:0263-6484
DOI:10.1002/cbf.290090409
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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8. |
Inhibitory action of melatonin and structurally related compounds on testosterone production by mouse leydig cellsin vitro |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 281-286
S. Persengiev,
J. Kehajova,
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摘要:
AbstractThe possible effect of melatinin, 5‐methoxytryptamine, 5‐methoxytryptophol, 6‐chloromelatonin and 2‐iodomelatonin on testosterone production by Leydig cellsin vitrowas investigated. The ability of individual indoles to inhibit testosterone production was found to depend on the concentration used. The relative inhibitory potency of the compounds tested was: 6‐chloromelationin>2‐iodomelatonin>melatonin>5‐methoxytryptamine>5‐methoxytryptophol. The results revealed that natural indoles which are synthesized in the pineal gland and their halogenized derivatives are capable of influencing directly testosterone production by Leydig cells. Also, these results demonstrated that melatonin exerts its remarkable antigonadotrophic effects, at least in part, through the direct decrease of testosterone production. Moreover, 6‐chloromelationin and 2‐iodomelatonin, which are reported to inhibit melatonin binding to target tissues, possess properties of biological melatonin analogues under the conditions of th
ISSN:0263-6484
DOI:10.1002/cbf.290090410
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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9. |
Cytophotometric assessment of Feulgen‐DNA and coomassie‐total cell protein in adrenocortical fasciculata cells of noise‐exposed wister rats |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 287-292
D. U. Ballough,
G. P. Ballough,
J. A. Strauss,
M. J. Durkot,
A. Anthony,
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摘要:
AbstractThe present investigation was undertaken to examine possible noise‐induced alterations in adrenal fasciculata cell (AFC) metabolism, and also to determine if the magnitude of these changes differs in male versus female rats. Wistar rats approximately 3 months old were exposed to intense noise for 60 min (100 dB, re 2 × 10−5N(m2)−1, 350–20 000 Hz); control rats were house under identical conditions, at an ambient noise level of 40–60 dB. Adrenal fasciculata cells (AFC) from each animal were examined for noise‐induced alterations in Feulgen‐DNA reactivity (as an indicator of chromatin template activity) and Coomassie‐total cell protein levels using scanning‐integrating cytophotometry. The results provide evidence that intense noise elicited a marked AFC metabolic enhancement in both male and female rats; the degree of this enhancement was more pronounced in males. This disparity may be due to pre‐existing differences in male versus female AFC enzymatic capability and subsequent responsiveness to nois
ISSN:0263-6484
DOI:10.1002/cbf.290090411
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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10. |
Histones and other basic nuclear proteins. S. Hnilica, G. S. Stein and J. L. Stein (eds). CRC Press: Florida, ISBN 0‐8493‐551 5‐X, 406 pages, $286 (1989) |
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Cell Biochemistry and Function,
Volume 9,
Issue 4,
1991,
Page 293-294
P. B. Gahan,
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ISSN:0263-6484
DOI:10.1002/cbf.290090412
出版商:John Wiley&Sons, Ltd.
年代:1991
数据来源: WILEY
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