ISSN:0263-6484    

DOI:10.1002/cbf.290130303

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290130304

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290130305

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290130306

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290130307

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290130308

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290130309

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290130310

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThe action of cyanide (500 μM), 2,4‐dinitrophenol (50 μM) and atractyloside (100 μM) on glycogen catabolism and oxygen uptake was investigated in the bivascularly perfused liver of fed rats. Cyanide, 2,4‐dinitrophenol and atractyloside were infused at identical rates into the hepatic artery in either the anterograde or retrograde perfusion. The accessible aqueous cell spaces were determined by means of the multiple‐indicator dilution technique. Glucose release, oxygen uptake and glycolysis were measured as metabolic parameters.Oxygen uptake changes per unit cell space caused by atractyloside (inhibition) and 2,4‐dinitrophenol (stimulation) were equal in the retrograde perfusion (periportal cells) and the anterograde perfusion (space enriched in perivenous cells); the decreases caused by cyanide were higher in the retrograde perfusion. Glucose release from periportal cells was not increased upon inhibition of oxidative phosphorylation, a phenomenon which was independent of the mechanism of action of the inhibitor. There were nearly identical changes in glycolysis in the periportal and perivenous cells.It was concluded that: (1) oxygen concentration in the perfused rat liver, if maintained above 100 μM, had little influence on the zonation of the respiratory activity; (2) in spite of the lower activities of the key enzymes of glycolysis in the periportal hepatocytes, as assayed under standard conditions, these cells were as effective as the perivenous ones in generating ATP in the cytosol when oxidative phosphorylation was impaired; (3) the key enzymes of glycogenolysis and glycolysis in periportal and perivenous cells responded differently to changes in the en

ISSN:0263-6484    

DOI:10.1002/cbf.290130311

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractCD36, a multifunctional adhesion receptor e.g. for thrombospondin and collagen, as well as a scavenger receptor for oxidized low density lipoprotein, is expressed e.g. on platelets and monocytes. By this dual role it might be involved in early steps of atherosclerosis like the recruitment of monocytes and formation of foam cells. We therefore studied the effects of n‐3 fatty acids on CD36 expression in human monocytic cells. Incorporation of eicosapentaenoic acid (EPA, C20:5n‐3) and docosahexaenoic acid (DHA, C22:6n‐3) into cellular phospholipids resulted in a significant reduction of CD36 expression at the mRNA and protein level, whereas arachidonic acid (AA, C20: 4n‐6) and linoleic acid (LA, C18:2n‐6) tended to increase CD36 expression compared to the control. This specific down‐regulation of CD36 by n‐3 fatty acids in cells involved in the initiation and progression of atherogenesis and inflammation, represents a further mechanism that may contribute to the beneficial effects of n‐3 polyunsaturated fatty acids (PUFA) in

ISSN:0263-6484    

DOI:10.1002/cbf.290130312

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY