ISSN:0263-6484    

DOI:10.1002/cbf.290040402

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290040403

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290040404

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractIn order to establish a method for studying myeloid differentiation, light density, non‐adherent, T‐cell depleted mononuclear cells prepared from 26 normal peripheral blood buffy‐coats were cultured in McCoy' 5A medium supplemented with 15 per cent fetal calf serum (FCS) for three weeks at 37°C in a humidified 5 per cent CO2atmosphere. The total number of viable cells in the cultures on weeks 1 and 2 represented 73 ± 10 per cent and 98 ± 41 per cent of the initial number of viable cells seeded. After one week, blasts represented 26 ± 10 per cent of the initial number of viable cells while all the initially contaminating mature granulocytes had disappeared. After two weeks, granulocytic differentiation was noted in most cultures and viable myelocytes and more mature cells represented 45 ± 26 per cent of the initial number of viable cells. The differentiation was independent on the lot of FCS used. The addition of PHA stimulated leukocyte conditioned medium to the cultures did not enhance granulocytic differentiation. The granulocytic differentiation observed in the absence of exogenous CSF persisted after removing the cells adhering to the bottom of the flasks on day 2 of the culture. An endogenous colony stimulating activity was detected in the cultures on week 3 but its intensity did not clearly correlate with the degree of granulocytic diffe

ISSN:0263-6484    

DOI:10.1002/cbf.290040405

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractRed blood cells exposedin vitroto phenylhydrazine acquired Heinz bodies, bound autologous IgG and were then phagocytized when incubated with autologus mononuclear phagocytes.In vivo, phenylhdyrazine administered to rabbits, caused the appearance of high plasma hemoglobin levels and hemoglobinuria as well as Heinz body formations and IgG binding to erythrocytes. This suggests that whilein vitrothe main mechanism of red cell removal seems to be phagocytoses,in vivoboth intravascular hemolysis and phagocytosis are active processes.Preliminary biochemical studies on phenylhydrazine‐exposed erythrocytes showed that together with the well‐known appearance of Heinz bodies, methemoglobin and a drop in reduced glutathione, this drug also causes ATP depletion. This is initially concomitant with the appearance of ADP and AMP and subsequently hypoxantine. Thus, irreversible ATP depletion may contribute to the genesis of the hemolytic process observedin v

ISSN:0263-6484    

DOI:10.1002/cbf.290040406

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractIsolated tubule cells from chick kidney respond to a short period of phosphate deprivation with increased phosphate uptake and a resistance to parathyroid hormone. During phosphate depletion a considerable amount of phosphate may be released from the cells, but intracellular inorganic phosphate levels are maintained by the hydrolysis of organic phosphate esters. It is suggested that the concomitant changes in metabolism might act as the signal causing the onset of the changes in phosphate handling associated with phosphate deprivation.

ISSN:0263-6484    

DOI:10.1002/cbf.290040407

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractThe pro‐drug cortisol succinate is frequently used as a substitute for cortisol in organ cultures. We found, however, that in Dulbecco's modified Eagle's medium the time taken for the ester to undergo 50 per cent hydrolysis (t½) to cortisol was 75 h. When 15 per cent heat‐inactivated normal rabbit serum was presentt½decreased to 47 h, but the rate of hydrolysis was not further increased in the presence of porcine articular cartilage or minced synovial tissue. When frozen and thawed synovium was presentt½decreased to 33 h, presumably due to the release of carboxyl‐esterases from the disrupted cells. The level of tetrahydrocortisol was low in all of the cultures. The slow hydrolysis of cortisol succinate resulted in the exposure of the tissues to undersirable fluctuations in the concentration of active hormone, which decreased to low levels at each medium change. Thus, in co‐cultures of porcine synovium and articular cartilage, cortisol had a greater inhibitory effect than cortisol succinate on the breakdown of cartilage matrix caused by synov

ISSN:0263-6484    

DOI:10.1002/cbf.290040408

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractNuclear, chromatin and microsomal fractions were isolated from hepatocytes prepared from rats injected with [32P] O 42−and killed subsequently at times between 1 and 48 h. Specific activities of the total phospholipids (PL) were determined for each subcellular fraction. The major points noted were (a) the initial specific activity of the chromatin PL was half that of both nuclear and microsomal PL at 1 h; (b) the first peak of labelling occurred at 6 h in both nuclear and microsomal PL, but was 3 h later (9h) in the chromatin PL; and (c) a second peak of labelling occurred in the chromatin and microsomal PL, but not in those of the nuclei.On fractionation of the PL, the major and most metabolically active components were phosphatidylcholine + phosphatidylethanolamine, whilst sphingomyelin accounted for only about 8 per cent of the total PL. The chromatin and microsomal fractions were somewhat similar in their labelling patterns though with a delayed peaking of activity in the chromatin. This is indicative of a synthesis and transport of PL from the microsomes to the chroma

ISSN:0263-6484    

DOI:10.1002/cbf.290040409

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractEffects of the antiarrhythmic drugs (propranolol, perhexiline maleate, lidoflazine and iproveratril) on energy‐linked reactions and on membrane potential were studied. Propranolol, perhexiline maleate and lidoflazine inhibit the ATPase activity of undamaged and broken mitochondria, and of submitochondrial particles. All drugs are inhibitors of either ATP‐driven or of succinate‐driven reduction of NADP+. The antiarrhythmics promote a decrease in the membrane potential upon energization of the mitochondrial membrane by α‐ketoglutarate, succinate, or ATP. It was suggested that these drugs have a primary action on the mitochondrial membrane, thus altering the activities of membrane proteins (channels and

ISSN:0263-6484    

DOI:10.1002/cbf.290040410

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290040411

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY