ISSN:0263-6484    

DOI:10.1002/cbf.290010202

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290010203

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290010204

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290010205

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290010206

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

摘要:

AbstractDispersed sensory ganglion cells from the embryonic chick, but not cells from other tissues, liberate biologically active material from nerve growth factor coupled to Sepharose beads. Experiments with such insolubilized material cannot then be taken as evidence for the existence of an external membrane receptor for the factor and may indicate that responsive cells have the ability to cleave an active fragment from the polypeptide.

ISSN:0263-6484    

DOI:10.1002/cbf.290010207

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

摘要:

AbstractThe spreading of HeLa cells on plastic substratum is mediated by fibronectin‐depleted foetal calf serum but not by fibronectin isolated by gelatin‐Sepharose affinity chromatography. The same is true for freshly explanted chick embryonic chondrocytes. In contrast, BHK cell spreading exceeds 67% after 120 min at 37°C in fibronectin‐supplemented (10 μg ml−1) serum‐free medium. Long‐term cultivation of HeLa cells in Eagle's MEM supplemented with fibronectin‐free serum is associated with the accumulation of cells in mitosis or before cytokinesis; many cells die and the remaining living cells, characterized by marked changes in morphology, multiply very slowly. It can be concluded therefore that fibronectin does not produce spreading in HeLa cells but forces th

ISSN:0263-6484    

DOI:10.1002/cbf.290010208

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

摘要:

AbstractQuantitative cytochemistry of components of blood neutrophil azurophilic granules (myeloperoxidase, chloroacetate esterase, β‐glucuronidase, and acid phosphatase) and specific granules (lactoferrin) has been performed by scanning and integrating micro‐densitometry in 13 patients with a myelodysplastic syndrome and 11 patients with chronic granulocytic leukaemia. Both patient groups showed a reduction of enzyme activity in azurophilic granules, and also of lactoferrin, consistent with abnormal development of neutrophil granules. These cytochemical changes in blood neutrophils are similar to those found in acute myeloid leukaemia, are consistent with a leukaemic maturation defect, and may be of diagnostic v

ISSN:0263-6484    

DOI:10.1002/cbf.290010209

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

摘要:

AbstractThe activity of rat liver tyrosine amino transferase (TAT) increases after hepatectomy with a first prominent peak at 8 h and a second peak at 18 h. This change in activity is probably due to de novo enzyme synthesis since it is prevented by actinomycin‐D (AMD). In the same period an increase of the lysosomal converting enzyme (convertase) which catalyses the in vitro transition of TAT from form I to form III, has been observed; this is not accompanied by changes of other lysosomal enzymes, such as acid phosphatase and cathepsin L. The activity of convertase is equal to that of the controls (sham operated animals) 2 h after hepatectomy, increases three times at 5 h, maintains the same value at 8 h and then decreases slowly to control level after 24 h. The correlation between the activity changes of the two enzymes strongly suggests a physiological role of convertase in TAT turnove

ISSN:0263-6484    

DOI:10.1002/cbf.290010210

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY

摘要:

AbstractChanges in the activities of several lysosomal enzymes were studied during transformation of mouse spleen cells in vitro. The activity of β‐glucuronidase increased during culture in the presence of T or B‐cell mitogens, and lymphoblasts contained higher levels of activity than did small, non‐transformed lymphocytes. Moreover, lymphoblasts in well‐transformed cultures had higher activities than those in poorly‐transformed cultures. The activities of other lysosomal enzymes (N‐acetyl‐β‐glucosaminidase, α‐mannosidase, β‐glucosidase) also increased during mitogenic stimulation, but each at different rates, although aryl sulphatase was unaffected. Such differences may be of importance when lymphocytes are used for diagnosis of inherited lysoso

ISSN:0263-6484    

DOI:10.1002/cbf.290010211

出版商:John Wiley&Sons, Ltd.    

年代:1983

数据来源: WILEY