ISSN:0263-6484    

DOI:10.1002/cbf.290020302

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290020303

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290020304

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290020305

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

ISSN:0263-6484    

DOI:10.1002/cbf.290020306

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractThis note describes an immunofluorescent staining method for cells in the S‐phase which have been allowed to take up bromodeoxyuridine into their DNA in place of thymine. The technique involves the use of fluorescinated monoclonal antibodies against bromodeoxyuridine and allows rapid and accurate estimation of cells in the S‐phase, the technique does not require interpretation by skilled technici

ISSN:0263-6484    

DOI:10.1002/cbf.290020307

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractA new technique for single‐step subcellular fractionation of adipose tissue homogenates by analytical sucrose density gradient centrifugation in a vertical pocket reorientating rotor is described. The density gradient distributions of mitochondrial and peroxisomal marker enzymes in brown and white adipose tissue of control and cold exposed rats are compared. The equilibrium density of brown fat mitochondria was found to be significantly increased compared with white fat mitochondria. GDP binding activity was localized solely to the mitochondria in both control and cold‐adapted brown adipose tissue. Brown and white fat mitochondria fractions were isolated by differential centrifugation and the specific activities of various enzymes in the homogenate and mitochondrial preparations determined. The specific activity of creatine kinase in brown adipose tissue was found to be ten‐fold higher than in white fat and subcellular fractionation studies showed the activity to have an exclusively cytosolic distribution in both tissues. GDP binding activity and some of the mitochondrial enzymes showed, in brown adipose, a striking increase in total activity in cold adapted rats compared to control animals. For some enzyme activities there was a small increase when expressed per mg tissue or per mg mitochondrial protein. When expressed per mg DNA i.e. per cell, there was a reduced specific activity of the mitochondrial and peroxisomal enzymes in both brown and white adipose tissue on cold adapt

ISSN:0263-6484    

DOI:10.1002/cbf.290020308

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractThe metabolic and secretory effects of methylamine in rat pancreatic islets were investigated. Methylamine accumulated in islet cells, was incorporated into endogenous islet proteins, and inhibited the incorporation of [2,5‐3H] histamine into eitherN,N‐dimethylcasein or endogenous islet proteins. Methylamine (2 mM) did not affect the oxidation of glucose or endogenous nutrients or the intracellular pH in islet cells. Glucose did not affect the activity of transglutaminase in islet homogenates, the uptake of14C‐methylamine by intact islets or its incorporation into endogenous islet proteins. Methylamine inhibited insulin release evoked by glucose, other nutrient secretagogues, and non‐nutrient insulinotropic agents such asL‐arginine or gliclazide. The inhibitory effect of methylamine upon insulin release was diminished in the presence of cytochalasin B or at low extracellular pH. Methylamine retarded the conversion of proinsulin to insulin. Trimethylamine (0.7 mM) was more efficiently taken up by islet cells than methylamine (2.0 mM), and yet caused only a modest inhibition of insulin release. These findings suggest that methylamine interferes with a late step in the secretory sequence, possibly by inhibiting the access of secretory granules to their exocyt

ISSN:0263-6484    

DOI:10.1002/cbf.290020309

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractAnalysis of free nucleotide composition of both avian thrombocytes and pig platelets showed quantitative differences in the level of adenine nucleotides.3H‐adenine taken up by turkey thrombocytes was metabolized mainly to adenine nucleotides was not released after thrombin action. Thrombin liberated non‐radioactive adenine nucleotides (18.2 ± 1.5 %, 20.6 ± 1.9%) of the total, probably localized in a storage pool. Malonyldialdehyhyde (MDA) production due to thrombin was observed in both platelets and thrombo

ISSN:0263-6484    

DOI:10.1002/cbf.290020310

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractIntact human erythrocytes were treated, under non‐haemolytic conditions at 37°C, with synthetic phosphatidylcholine which has homologous, saturated acyl chains of 8 ∼ 18 even‐numbered carbon atoms (C8∼ C18‐PC) or with lysophosphatidylcholine which has a saturated acyl chain of 8 ∼ 18 carbon atoms (C8∼ C18‐lysoPC). The C8∼ C14‐PC and C12∼ C18‐lysoPC species were rapidly incorporated into the erythrocytes and induced a shape change of the crenation (echinocyte formation) type. The site of the incorporation was found to be most probably on the outer leaflet of the membrane lipid bilayer. The extent of the shape change was dependent on the amount of each lipid incorporated. When the same amount of a PC or lysoPC species was incorporated into the membrane, about the same extent of crenation was induced, independent of acyl chain length. However, C16‐PC, C18‐PC, C8‐lysoPC and C10‐lysoPC, which were not incorporated into the erythrocytes, did not induce any shape change. It is therefore suggested that the hydrophobic moiety of these amphiphilic lipids may greatly contribute to their transfer from the outer medium into the erythrocyte membrane, but do not influence so much the perturbation of the membrane lipid bilayer which may be responsible f

ISSN:0263-6484    

DOI:10.1002/cbf.290020311

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY